Cloned (Comment) | Organism |
---|---|
- |
Lactobacillus phage phigaY |
Protein Variants | Comment | Organism |
---|---|---|
D12A | cell-lytic activity is strongly reduced | Lactobacillus phage phigaY |
D12A/E33A | no cell-lytic activity | Lactobacillus phage phigaY |
D12G | cell-lytic activity isstrongly reduced | Lactobacillus phage phigaY |
D198A | cell-lytic activity is slightly reduced | Lactobacillus phage phigaY |
D36A | cell-lytic activity is similar to wild-type enzyme | Lactobacillus phage phigaY |
D96A | cell-lytic activity is strongly reduced | Lactobacillus phage phigaY |
E237A | cell-lytic activity is similar to wild-type enzyme | Lactobacillus phage phigaY |
E238A | cell-lytic activity is similar to wild-type enzyme | Lactobacillus phage phigaY |
E33A | cell-lytic activity isstrongly reduced | Lactobacillus phage phigaY |
E88D | cell-lytic activity is similar to wild-type enzyme | Lactobacillus phage phigaY |
E98A | no cell-lytic activity | Lactobacillus phage phigaY |
G10S | cell-lytic activity is strongly reduced | Lactobacillus phage phigaY |
G253A | cell-lytic activity is similar to wild-type enzyme | Lactobacillus phage phigaY |
G267A | cell-lytic activity is similar to wild-type enzyme | Lactobacillus phage phigaY |
G281A | cell-lytic activity is similar to wild-type enzyme | Lactobacillus phage phigaY |
G292A | cell-lytic activity is similar to wild-type enzyme | Lactobacillus phage phigaY |
H60R | cell-lytic activity is strongly reduced | Lactobacillus phage phigaY |
K142E | cell-lytic activity is similar to wild-type enzyme | Lactobacillus phage phigaY |
K142R | cell-lytic activity is similar to wild-type enzyme | Lactobacillus phage phigaY |
K207A | cell-lytic activity is similar to wild-type enzyme | Lactobacillus phage phigaY |
K211A | cell-lytic activity is similar to wild-type enzyme | Lactobacillus phage phigaY |
K25T | cell-lytic activity is similar to wild-type enzyme | Lactobacillus phage phigaY |
L132P | cell-lytic activity is strongly reduced | Lactobacillus phage phigaY |
L264A | cell-lytic activity is slightly reduced | Lactobacillus phage phigaY |
M1I | cell-lytic activity is reduced | Lactobacillus phage phigaY |
M1K | cell-lytic activity is reduced | Lactobacillus phage phigaY |
additional information | deletion analysis demonstrates that the beta/alphagaY domain of N-terminal 216 residues is the core enzyme portion, although the cell-lytic ability is lower than that of LysgaY. These mutational experiments suggest that beta/alphagaY (in which two acidic residues of D12 and E98 likely act as catalytic residues) is responsible for cell-lytic activity, and SH3bgaY promotes beta/alphagaY possibly through cell-wall binding function | Lactobacillus phage phigaY |
N67K | cell-lytic activity is similar to wild-type enzyme | Lactobacillus phage phigaY |
P212A | cell-lytic activity is similar to wild-type enzyme | Lactobacillus phage phigaY |
P216A | cell-lytic activity is similar to wild-type enzyme | Lactobacillus phage phigaY |
R109L | cell-lytic activity is similar to wild-type enzyme | Lactobacillus phage phigaY |
R251A | cell-lytic activity is slightly reduced | Lactobacillus phage phigaY |
V124M | cell-lytic activity is slightly reduced | Lactobacillus phage phigaY |
V79F | cell-lytic activity is similar to wild-type enzyme | Lactobacillus phage phigaY |
W284A | cell-lytic activity is strongly reduced | Lactobacillus phage phigaY |
W284G | cell-lytic activity is strongly reduced | Lactobacillus phage phigaY |
Y272A | cell-lytic activity is strongly reduced | Lactobacillus phage phigaY |
Y61H | cell-lytic activity is stronly reduced | Lactobacillus phage phigaY |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co(NO3)2 | 10 mM | Lactobacillus phage phigaY | |
CoCl2 | 10 mM | Lactobacillus phage phigaY | |
CuSO4 | 10 mM | Lactobacillus phage phigaY | |
NiSO4 | 10 mM | Lactobacillus phage phigaY | |
Zn(NO3)2 | 10 mM | Lactobacillus phage phigaY | |
ZnCl2 | 10 mM | Lactobacillus phage phigaY |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactobacillus phage phigaY | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
peptidoglycan + H2O | LysgaY lysed over 20 heated Gram-positive bacterial species as the substrates, including lactobacilli, lactococci, enterococci, micrococci, and staphylococci | Lactobacillus phage phigaY | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
endolysin | - |
Lactobacillus phage phigaY |
LysgaY | - |
Lactobacillus phage phigaY |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Lactobacillus phage phigaY |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Lactobacillus phage phigaY |