Cloned (Comment) | Organism |
---|---|
expression of the enzyme in transgenic cattle, Bos taurus, as secreted enzyme in milk using the pBC2-HLY-NEOR transgene vector | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Homo sapiens | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from transgenic cow milk, from which fat and casein has been removed for purification of the enzyme, by cation exchange chromatography and gel filtration to over 95% purity | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
milk | - |
Homo sapiens | - |
Subunits | Comment | Organism |
---|---|---|
? | x * 14679.14, mass spectrometry | Homo sapiens |
More | peptide mass fingerprinting of recombinant enzymes, overview | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
HLZ | - |
Homo sapiens |
muramidase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
recombinant enzyme | Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
purified recombinant enzyme, 45 min, 95% activity remaining | Homo sapiens |
100 | - |
purified recombinant enzyme, 10 min, 50% activity remaining | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 7 | the optimal pH of rHLZ varies with salt concentration of the buffer, recombinant enzyme | Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | activity range, recombinant enzyme | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
additional information | the modified bovine milk is a possible substitute for human milk | Homo sapiens |