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3.2.1.17: lysozyme

This is an abbreviated version!
For detailed information about lysozyme, go to the full flat file.

Word Map on EC 3.2.1.17

Reaction

N,N',N'',N'''-tetraacetylchitotetraose
+
H2O
=
N,N',N''-triacetylchitotriose
 +
N-acetyl-D-glucosamine

Synonyms

1,4-beta-N-acetylmuramidase, 1,4-beta-N-acetylmuramidase 1, 1,4-beta-N-acetylmuramidase A/C, 1,4-beta-N-acetylmuramidase M1, 1,4-beta-N-acetylmuramoylhydrolase, 1,4-N-acetylmuramidase, abLysI, AcmB, ASTL, Autolysin, BA-lysozyme, c-type lysozyme, CCLys-g, CFL, chicken-type lysozyme, cLys3, CP-1 lysin, CP-7 lysin, CP-9 lysin, CPL, DEL-I, DEL-II, DEL-III, destabilase-lysozyme, EGL, Egyptian goose egg-white lysozyme, Egyptian goose lysozyme, endolysin, EQL, equine lysozyme, fOg44 endolysin, g-lysozyme, g-type lysozyme, GEWL, globulin G, globulin G1, goose type lysozyme, Goose-type lysozyme, gp144, gp36C, GSTL, HEWL, HLysG2, hLYZ, HLZ, i-type lysozyme, iLys, iLys2, invertebrate lysozyme, invertebrate type lysozyme, L-7001, LAL, lambda lysozyme, Late protein gp15, Lys-rich lysozyme 2, lysC, LYSC-1, LysG, LysgaY, LysGL, lysi1, lysi2, Lysis protein, Lysosyme, lysozym, Lysozyme, lysozyme 1, lysozyme 1 precursor, lysozyme A, lysozyme B, lysozyme c, lysozyme c-1, lysozyme g, lysozyme g-like 2, lysozyme SP0987, lysozyme-g, LYZ, LYZ2, M1L, Mdl1, MdL2, mlD-Ds1, mlD-Ds2, mlD-Ds3, MLL-A, MLL-B, mucopeptide glucohydrolase, mucopeptide N-acetylmuramic acid hydrolase, mucopeptide N-acetylmuramoylhydrolase, muramidase, mutanolysin, MV1 lysin, N,O-diacetylmuramidase, N-acetylglucosaminidase autolysin, N-acetylmuramic hydrolase, N-acetylmuramide glycanhydrolase, n-SalC, n-SalG, OEL, OHLysG1, OHLysG2, OHLysG3, Outer wedge of baseplate protein, P13, PALysG, Peptidoglycan hydrolase, peptidoglycan n-acetylmuramic hydrolase, phage-type like lysozyme, phiKZ endolysin, Ply3626, PR1-lysozyme, Protein gp17, Protein gp19, Protein Gp25, Protein Gp5, Protein gp54, Protein gpK, SalG, SjLys, SmLysC, SSTL A, SSTL B, T4 lysozyme, T4L, TJL, transglycosylase, VpLYZ, WGL, wood duck lysozyme

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.17 lysozyme

Temperature Stability

Temperature Stability on EC 3.2.1.17 - lysozyme

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
10 - 40
-
the enzyme remains stable for 30 min at 10-40°C, activity sharply decreases to about 15% after 30 min at 50°C
120
-
at 2 bar, 20 min, 79% loss of activity
20 - 30
20 - 70
-
unaffected, 10 min, pH 5.0
20 - 90
-
stable temperature-range
25
-
pH 3.2, half-life for the free enzyme is 39 h, for the enzyme immobilized on tosyl-activated and carboxylated micro-size magnetic particles is 280 h and 134 h, respectively
25.3
-
loss of 50% activity
30
10 min, 14% loss of activity
45
Tequatrovirus T4
-
inactivation above, 10 min
45.3
-
melting temperature, mutant C4S/C18S/C29S/C60S
45.4
-
thermal denaturation of the catalytic module
46.2
-
melting temperature, reduced wild-type
51.1
-
melting temperature, mutant C4S/C60S
53
-
50% inactivation at
54.3
-
melting temperature, mutant C18S/C29S
57
-
Tm-value for mutant E73A is 57.4°C. Tm-value for mutant E73Q is 56.6°C
60 - 100
-
15 min of treatment at 100°C disrupts almost all the antibacterial activity of the recombinant enzyme. Under 80°C, the recombinant enzyme can maintain about 60% of its activity for 15 min, and quickly loses the antibacterial activity after that. After incubation at 60°C for 45 min, the recombinant enzyme keeps 60% of its activity
60 - 70
-
10 min, about 40% loss of activity
60.6
-
melting temperature, wild-type
61
-
Tm-value for wild-type enzyme is 60.6°C
62.8
Tm value, pH 2.0
76
-
mid-transition temperature is 76.3°C for S6K-lysozyme and 76.0°C for S7-lysozyme
77
-
mid-transition temperature of wild-type enzyme
78 - 90
melting temperature at pH 3.0. The enzyme hydrolyzes at 90°C
85
-
purified enzyme, pH 6.2, inactivation after 60 min
95
1 h, 38.6% residual activity in absence of 2-mercaptoethanol, 93.8% residual activity in presence of 2-mercaptoethanol
additional information