3.2.1.166: heparanase
This is an abbreviated version!
For detailed information about heparanase, go to the full flat file.
Word Map on EC 3.2.1.166
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3.2.1.166
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metastasis
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proteoglycans
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angiogenesis
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endothelial
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heparin
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endoglycosidase
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basement
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glycosaminoglycans
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melanoma
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vessel
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oligosaccharide
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platelet
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syndecan-1
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hspgs
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node
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angiogenic
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myeloma
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nephropathy
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anticoagulant
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glycocalyx
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clinicopathological
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metalloproteinases
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glomerular
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procoagulant
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heparin-binding
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antimetastatic
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heparinase
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neovascularization
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fgf-2
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extravasation
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bfgf
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perlecan
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subendothelial
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matrigel
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microvessel
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chondroitin
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pro-tumorigenic
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o-sulfation
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matrix-degrading
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vegf-c
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pharmacology
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glypicans
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diagnostics
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proangiogenic
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medicine
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non-anticoagulant
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iduronic
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sdc1
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prometastatic
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chondroitinase
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intrachain
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drug development
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tfpi-2
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analysis
- 3.2.1.166
- metastasis
- proteoglycans
- angiogenesis
- endothelial
- heparin
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endoglycosidase
-
basement
- glycosaminoglycans
- melanoma
- vessel
- oligosaccharide
- platelet
- syndecan-1
-
hspgs
- node
-
angiogenic
- myeloma
- nephropathy
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anticoagulant
-
glycocalyx
-
clinicopathological
- metalloproteinases
- glomerular
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procoagulant
-
heparin-binding
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antimetastatic
- heparinase
- neovascularization
- fgf-2
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extravasation
- bfgf
- perlecan
-
subendothelial
- matrigel
-
microvessel
- chondroitin
-
pro-tumorigenic
-
o-sulfation
-
matrix-degrading
- vegf-c
- pharmacology
-
glypicans
- diagnostics
-
proangiogenic
- medicine
-
non-anticoagulant
-
iduronic
- sdc1
-
prometastatic
- chondroitinase
-
intrachain
- drug development
-
tfpi-2
- analysis
Reaction
endohydrolysis of (1->4)-beta-D-glycosidic bonds of heparan sulfate chains in heparan sulfate proteoglycan =
Synonyms
BpHep, C1A heparanase, endo-beta-D-glucuronidase, endo-beta-glucuronidase, heparan sulfate glycosidase, heparanase, heparanase 1, heparanase-1, HPA, Hpa1, Hpa1 heparanase, HPSE, T5
ECTree
3.2.1.166 heparanaseAdvanced search results
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results (Cloned
Cloned on EC 3.2.1.166 - heparanase
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CLONED (Commentary) 
ORGANISM
UNIPROT
LITERATURE
expression of an N-terminally His-tagged truncated enzyme form, residues 36-543, containing a TEV protease cleavage site, in Escherichia coli strain BL21-CodonPlus (DE3)-RIL
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expression of the cloned cDNA in insect and mammalian cells yields 65000 Da and 50000 Da recombinant heparanase proteins. The 50000 Da enzyme represents an N-terminally processed enzyme, at least 100fold more active than the 65000 Da form
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expression of the enzyme in Spodoptera frugiperda Sf9 cells and secretion to the cell culture medium
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insect cell expression system is used for the generation of large amounts of recombinant protein of high specific activity. Individual subunits are cloned into baculoviral secretory vectors and coexpressed in insect cells
NIH3T3 and COS-7 cells stably transfected with pBK-CMV expression vectors
recombinant expression of GST-tagged enzyme from vector pGEX-2TK-heparanase in Escherichia coli strain BL21
recombinant expression of His-tagged smaller 8 kDa subunit in Escherichia coli strain BL21 (DE3)plysS
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recombinant expression of the His-tagged enzyme from pET-15b expression vector containing a TEV cleavage site in Escherichia coli BL21-DE3 codon plus
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single gene, recombinant expression in Spodoptera frugiperda cells via baculovrius transfection method. The two subunits cotranslationally fold into mature heterodimeric HPSE, bypassing the 65 kDa proenzyme form and ensuring expression of only active enzyme
