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3.2.1.166: heparanase

This is an abbreviated version!
For detailed information about heparanase, go to the full flat file.

Word Map on EC 3.2.1.166

Reaction

endohydrolysis of (1->4)-beta-D-glycosidic bonds of heparan sulfate chains in heparan sulfate proteoglycan =

Synonyms

BpHep, C1A heparanase, endo-beta-D-glucuronidase, endo-beta-glucuronidase, heparan sulfate glycosidase, heparanase, heparanase 1, heparanase-1, HPA, Hpa1, Hpa1 heparanase, HPSE, T5

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.166 heparanase

Purification

Purification on EC 3.2.1.166 - heparanase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
purifiecation of the His-tagged smaller subunit fragment of the human heparanase to homogeneity by immobilized metal affinity chromatography and dialysis from Escherichia coli strain BL21 (DE3)plysS
-
recombinant enzyme from insect cells
recombinant GST-tagged enzyme from Escherichia coli strain BL21 by glutathione affinity chromatography
recombinant His-tagged enzyme from Escherichia coli BL21-DE3 codon plus by metal affinity chromatography
-
recombinant myc-DDK-tagged proheparanase partially by tangential ultrafiltration
recombinant N-terminally His-tagged truncated enzyme form from Escherichia coli strain BL21-CodonPlus (DE3)-RIL by nickel affinity chromatography and dialysis
-