Literature summary for 3.2.1.166 extracted from

Peterson, S.B.; Liu, J.
Unraveling the specificity of heparanase utilizing synthetic substrates (2010), J. Biol. Chem., 285, 14504-14513.

Search for more literature for 3.2.1.166

Cloned(Commentary)

Cloned (Comment)	Organism
expression in CHO cells	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
(IdoA2S-GlcNS)n	polysaccharide containing IdoA2S-GlcNS repeating unit, inhibits the activity of heparanase	Homo sapiens
additional information	polysacchride with repeating disaccharide units of GlcA-GlcNAc or polysaccharide with repeating units of GlcA-GlcNAc6S do not inhibit activity of heparanase	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q9Y251	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	the substrate that carries the repeating unit of IdoA2S-GlcNS is completely resistant to the heparanase digestion. The polysaccharide with repeating units of GlcA-GlcNAc6S is not a a substrate. No activity with heparosan	Homo sapiens	?	-	?
[GlcAbeta(1,4)GlcNSO3H-3SO3Halpha(1,4)]m-GlcAbeta(1,4)GlcNSO3H-3SO3Halpha(1,4)-[GlcAbeta(1,4)GlcNSO3H-3SO3H]n + H2O	both N- and O-sulfations (either 6-O-sulfation or 3-O-sulfation) are required for the cleavage by heparanase. Structural moiety recognized by heparanase includes a GlcA unit and GlcNS unit carrying O-sulfations. The enzyme proves to be promiscuous in the aspect of the type and location of O-sulfation required for recognition	Homo sapiens	[GlcAbeta(1,4)GlcNSO3H-3SO3Halpha(1,4)]m-GlcA + GlcNSO3H-3SO3Halpha(1,4)-[GlcAbeta(1,4)GlcNSO3H-3SO3H]n	-	?
[GlcAbeta(1,4)GlcNSO3H-6SO3Halpha(1,4)]m-GlcAbeta(1,4)GlcNSO3H-6SO3Halpha(1,4)-[GlcAbeta(1,4)GlcNSO3H-6SO3H]n + H2O	both N- and O-sulfations (either 6-O-sulfation or 3-O-sulfation) are required for the cleavage by heparanase. Structural moiety recognized by heparanase includes a GlcA unit and GlcNS unit carrying O-sulfations. The enzyme proves to be promiscuous in the aspect of the type and location of O-sulfation required for recognition	Homo sapiens	[GlcAbeta(1,4)GlcNSO3H-6SO3Halpha(1,4)]m-GlcA + GlcNSO3H-6SO3Halpha(1,4)-[GlcAbeta(1,4)GlcNSO3H-3SO3H]n	-	?
[GlcAbeta(1,4)GlcNSO3Halpha(1,4)]m-GlcAbeta(1,4)GlcNSO3Halpha(1,4)-[GlcAbeta(1,4)GlcNSO3H]n + H2O	heparanase does not cleave the linkage of GlcA2S-GlcNS but rather cleaves the linkage of GlcA-GlcNS nearby	Homo sapiens	[GlcAbeta(1,4)-GlcNSO3Halpha(1,4)]m-GlcA + GlcNSO3Halpha(1,4)-[GlcAbeta(1,4)GlcNSO3H]n	-	?

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6	-	assay at	Homo sapiens

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Release 2023.1 (January 2023)