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Literature summary for 3.2.1.166 extracted from

  • Miao, H.Q.; Navarro, E.; Patel, S.; Sargent, D.; Koo, H.; Wan, H.; Plata, A.; Zhou, Q.; Ludwig, D.; Bohlen, P.; Kussie, P.
    Cloning, expression, and purification of mouse heparanase (2002), Protein Expr. Purif., 26, 425-431.
    View publication on PubMed
Search for more literature for 3.2.1.166

Cloned(Commentary)

Cloned (Comment) Organism
stably expressed in NS0 myeloma cells Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
heparin
-
 Mus musculus
laminarin sulfate 0.01 mM, complete inhibition Mus musculus
suramin
-
 Mus musculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
8000
-
 1 * 50000 + 1 * 8000, non-covalently bound heterodimer of the 50000 Da protein (Lys150–Ile535) and the 8000 Da peptide (Asp28–Lys100), SDS-PAGE Mus musculus
50000
-
 1 * 50000 + 1 * 8000, non-covalently bound heterodimer of the 50000 Da protein (Lys150–Ile535) and the 8000 Da peptide (Asp28–Lys100), SDS-PAGE Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
embryo
-
 Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
heparan sulfate proteoglycan + H2O
-
 Mus musculus heparan sulfate fragment + truncated heparan sulfate proteoglycan
-
 ?

Subunits

Subunits Comment Organism
heterodimer 1 * 50000 + 1 * 8000, non-covalently bound heterodimer of the 50000 Da protein (Lys150–Ile535) and the 8000 Da peptide (Asp28–Lys100), SDS-PAGE Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Mus musculus

pH Range

pH Minimum pH Maximum Comment Organism
5 7.5 pH 5.0: maximal activity, pH 7.5: almost 10-fold more enzyme is necessary to achieve activity comparable to pH 5. Little or no enzymatic activity is detected at pH 4.0 or pH 8.0 Mus musculus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00005
-
-
 Mus musculus laminarin sulfate
0.001
-
-
 Mus musculus heparin
0.05
-
-
 Mus musculus suramin