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3.2.1.106: mannosyl-oligosaccharide glucosidase

This is an abbreviated version!
For detailed information about mannosyl-oligosaccharide glucosidase, go to the full flat file.

Word Map on EC 3.2.1.106

Reaction

Glc3Man9GlcNAc2-[protein]
+
H2O
=
Glc2Man9GlcNAc2-[protein]
+
beta-D-glucopyranose

Synonyms

(Glc3)-glucosidase, acid alpha-glucosidase, alpha-glucosidase I, CHO-GAA, CWH41, Cwh41p, GAA, GCSI, Glc I, Glc3 oligosaccharide glucosidase, Glc3-glucosidase, Glc3-oligosaccharide glucosidase, Glc3-OS-glucosidase, Glc3Man9GlcNAc2 oligosaccharide glucosidase, Glc3Man9NAc2 oligosaccharide glucosidase, glucosidase 1, glucosidase I, glucosidase, mannosyloligosaccharide, glucosidase-1, glycoengineered acid alpha-glucosidase, HP-GAA, K12 YgjK protein, KNOPF, mannosyl-oligosaccharide glucosidase, mannosylglycerate hydrolase, MOGS, oxime-neo-rhGAA, PGI, processing alpha-glucosidase I, processing exoglucosidase I, processing glucosidase I, rhGAA, tgGAA, trimming enzyme I, trimming glucosidase I, Tt8MGH, YgjK

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.106 mannosyl-oligosaccharide glucosidase

Purification

Purification on EC 3.2.1.106 - mannosyl-oligosaccharide glucosidase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
DEAE Bio-Gel A column chromatography and Sephadex G-25 gel filtration
glutathione-agarose column chromatography, gel filtration
HiPrep 16/10 Phenyl FF column, dialysis against 20 mM Tris-HCl pH 7.5, Q-Sepharose 16/10 anion exchange column
-
lactating mammary gland
-
partial, calf
-
partial, soluble glucosidase I
-
truncated form Cwht1p purified as a soluble non-glycosylated protein to approximately 90% homogeneity by a combination of ammonium sulfate precipitation, anion-exchange chromatography and immobilized metal affinity chromatography
-