Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-deoxynojirimycin | strong inhibition | Bos taurus | |
1-deoxynojirimycin | strong inhibition | Saccharomyces cerevisiae | |
kojibiose | alpha-1,2-linked glucose disaccharide | Bos taurus | |
kojibiose | alpha-1,2-linked glucose disaccharide | Saccharomyces cerevisiae | |
additional information | - |
Bos taurus | |
additional information | no inhibition by 1 mM EDTA or 1 mM o-phenanthroline | Saccharomyces cerevisiae | |
N-(5-carboxypentyl)-1-deoxynojirimycin | strong inhibition | Bos taurus | |
N-(5-carboxypentyl)-1-deoxynojirimycin | strong inhibition | Saccharomyces cerevisiae | |
N-methyl-1-deoxynojirimycin | strong inhibition | Bos taurus | |
N-methyl-1-deoxynojirimycin | strong inhibition | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Saccharomyces cerevisiae | - |
- |
microsome | - |
Bos taurus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | metal ions not required | Saccharomyces cerevisiae | |
additional information | metal ions not required | Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
83000 | - |
x * 83000, SDS-PAGE, reducing conditions | Bos taurus |
95000 | - |
x * 95000, SDS-PAGE, reducing conditions | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Glc3Man9GlcNAc2 + H2O | Saccharomyces cerevisiae | hydrolyses specifically terminal alpha1,2-linked glucose residue | ? | - |
? | |
Glc3Man9GlcNAc2 + H2O | Bos taurus | hydrolyses specifically terminal alpha1,2-linked glucose residue | ? | - |
? | |
Glc3Man9GlcNAc2 + H2O | Saccharomyces cerevisiae | specific involvement in the N-linked oligosaccharide-processing pathway | ? | - |
? | |
Glc3Man9GlcNAc2 + H2O | Bos taurus | specific involvement in the N-linked oligosaccharide-processing pathway | ? | - |
? | |
additional information | Saccharomyces cerevisiae | involved in the formation of high mannose and complex glycoproteins | ? | - |
? | |
additional information | Bos taurus | involved in the formation of high mannose and complex glycoproteins | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
calf | - |
Saccharomyces cerevisiae | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | with high-mannose oligosaccharides | Saccharomyces cerevisiae |
glycoprotein | with high-mannose oligosaccharides | Bos taurus |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | calf | Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Saccharomyces cerevisiae |
additional information | - |
- |
Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Glc3Man9GlcNAc2 + H2O | hydrolyses specifically terminal alpha1,2-linked glucose residue | Saccharomyces cerevisiae | D-glucose + Glc2Man9GlcNAc2 | - |
? | |
Glc3Man9GlcNAc2 + H2O | hydrolyses specifically terminal alpha1,2-linked glucose residue | Bos taurus | D-glucose + Glc2Man9GlcNAc2 | - |
? | |
Glc3Man9GlcNAc2 + H2O | hydrolyses specifically terminal alpha1,2-linked glucose residue | Saccharomyces cerevisiae | ? | - |
? | |
Glc3Man9GlcNAc2 + H2O | hydrolyses specifically terminal alpha1,2-linked glucose residue | Bos taurus | ? | - |
? | |
Glc3Man9GlcNAc2 + H2O | specific involvement in the N-linked oligosaccharide-processing pathway | Saccharomyces cerevisiae | ? | - |
? | |
Glc3Man9GlcNAc2 + H2O | specific involvement in the N-linked oligosaccharide-processing pathway | Bos taurus | ? | - |
? | |
additional information | also acts, more slowly, on the corresponding glycolipids and glycopeptides | Saccharomyces cerevisiae | ? | - |
? | |
additional information | also acts, more slowly, on the corresponding glycolipids and glycopeptides | Bos taurus | ? | - |
? | |
additional information | p-nitrophenyl-alpha-D-glucopyranoside: not a substrate | Saccharomyces cerevisiae | ? | - |
? | |
additional information | p-nitrophenyl-alpha-D-glucopyranoside: not a substrate | Bos taurus | ? | - |
? | |
additional information | aryl-alpha-D-glucosides: not a substrate | Saccharomyces cerevisiae | ? | - |
? | |
additional information | aryl-alpha-D-glucosides: not a substrate | Bos taurus | ? | - |
? | |
additional information | Glc2Man9GlcNAc2: not a substrate | Saccharomyces cerevisiae | ? | - |
? | |
additional information | Glc2Man9GlcNAc2: not a substrate | Bos taurus | ? | - |
? | |
additional information | involved in the formation of high mannose and complex glycoproteins | Saccharomyces cerevisiae | ? | - |
? | |
additional information | involved in the formation of high mannose and complex glycoproteins | Bos taurus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 95000, SDS-PAGE, reducing conditions | Saccharomyces cerevisiae |
? | x * 83000, SDS-PAGE, reducing conditions | Bos taurus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Saccharomyces cerevisiae |
37 | - |
assay at | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.7 | - |
calf | Bos taurus |
6.8 | - |
pH optimum close to | Saccharomyces cerevisiae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.2 | 7.2 | half-maximal activity at | Saccharomyces cerevisiae |