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Literature summary for 3.2.1.106 extracted from

  • Bause E.; Erkens, R.; Schweden, J.; Jaenicke, L.
    Purification and characterization of trimming glucosidase I from Saccharomyces cerevisiae (1986), FEBS Lett., 206, 208-212.
No PubMed abstract available
Search for more literature for 3.2.1.106

Inhibitors

Inhibitors Comment Organism Structure
1-deoxynojirimycin strong inhibition Bos taurus
1-deoxynojirimycin strong inhibition Saccharomyces cerevisiae
kojibiose alpha-1,2-linked glucose disaccharide Bos taurus
kojibiose alpha-1,2-linked glucose disaccharide Saccharomyces cerevisiae
additional information
-
 Bos taurus
additional information no inhibition by 1 mM EDTA or 1 mM o-phenanthroline Saccharomyces cerevisiae
N-(5-carboxypentyl)-1-deoxynojirimycin strong inhibition Bos taurus
N-(5-carboxypentyl)-1-deoxynojirimycin strong inhibition Saccharomyces cerevisiae
N-methyl-1-deoxynojirimycin strong inhibition Bos taurus
N-methyl-1-deoxynojirimycin strong inhibition Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Saccharomyces cerevisiae
-
-
microsome
-
 Bos taurus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information metal ions not required Saccharomyces cerevisiae
additional information metal ions not required Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
83000
-
 x * 83000, SDS-PAGE, reducing conditions Bos taurus
95000
-
 x * 95000, SDS-PAGE, reducing conditions Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Glc3Man9GlcNAc2 + H2O Saccharomyces cerevisiae hydrolyses specifically terminal alpha1,2-linked glucose residue ?
-
 ?
Glc3Man9GlcNAc2 + H2O Bos taurus hydrolyses specifically terminal alpha1,2-linked glucose residue ?
-
 ?
Glc3Man9GlcNAc2 + H2O Saccharomyces cerevisiae specific involvement in the N-linked oligosaccharide-processing pathway ?
-
 ?
Glc3Man9GlcNAc2 + H2O Bos taurus specific involvement in the N-linked oligosaccharide-processing pathway ?
-
 ?
additional information Saccharomyces cerevisiae involved in the formation of high mannose and complex glycoproteins ?
-
 ?
additional information Bos taurus involved in the formation of high mannose and complex glycoproteins ?
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
 calf
-
Saccharomyces cerevisiae
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein with high-mannose oligosaccharides Saccharomyces cerevisiae
glycoprotein with high-mannose oligosaccharides Bos taurus

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharomyces cerevisiae

Source Tissue

Source Tissue Comment Organism Textmining
liver calf Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Saccharomyces cerevisiae
additional information
-
-
 Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Glc3Man9GlcNAc2 + H2O hydrolyses specifically terminal alpha1,2-linked glucose residue Saccharomyces cerevisiae D-glucose + Glc2Man9GlcNAc2
-
 ?
Glc3Man9GlcNAc2 + H2O hydrolyses specifically terminal alpha1,2-linked glucose residue Bos taurus D-glucose + Glc2Man9GlcNAc2
-
 ?
Glc3Man9GlcNAc2 + H2O hydrolyses specifically terminal alpha1,2-linked glucose residue Saccharomyces cerevisiae ?
-
 ?
Glc3Man9GlcNAc2 + H2O hydrolyses specifically terminal alpha1,2-linked glucose residue Bos taurus ?
-
 ?
Glc3Man9GlcNAc2 + H2O specific involvement in the N-linked oligosaccharide-processing pathway Saccharomyces cerevisiae ?
-
 ?
Glc3Man9GlcNAc2 + H2O specific involvement in the N-linked oligosaccharide-processing pathway Bos taurus ?
-
 ?
additional information also acts, more slowly, on the corresponding glycolipids and glycopeptides Saccharomyces cerevisiae ?
-
 ?
additional information also acts, more slowly, on the corresponding glycolipids and glycopeptides Bos taurus ?
-
 ?
additional information p-nitrophenyl-alpha-D-glucopyranoside: not a substrate Saccharomyces cerevisiae ?
-
 ?
additional information p-nitrophenyl-alpha-D-glucopyranoside: not a substrate Bos taurus ?
-
 ?
additional information aryl-alpha-D-glucosides: not a substrate Saccharomyces cerevisiae ?
-
 ?
additional information aryl-alpha-D-glucosides: not a substrate Bos taurus ?
-
 ?
additional information Glc2Man9GlcNAc2: not a substrate Saccharomyces cerevisiae ?
-
 ?
additional information Glc2Man9GlcNAc2: not a substrate Bos taurus ?
-
 ?
additional information involved in the formation of high mannose and complex glycoproteins Saccharomyces cerevisiae ?
-
 ?
additional information involved in the formation of high mannose and complex glycoproteins Bos taurus ?
-
 ?

Subunits

Subunits Comment Organism
? x * 95000, SDS-PAGE, reducing conditions Saccharomyces cerevisiae
? x * 83000, SDS-PAGE, reducing conditions Bos taurus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Saccharomyces cerevisiae
37
-
 assay at Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.7
-
 calf Bos taurus
6.8
-
 pH optimum close to Saccharomyces cerevisiae

pH Range

pH Minimum pH Maximum Comment Organism
6.2 7.2 half-maximal activity at Saccharomyces cerevisiae