Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Candida albicans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-deoxynojirimycin | - |
Candida albicans | |
australine | 39% inhibition at 0.02 mM | Candida albicans | |
bromoconduritol | 22% inhibition at 0.02 mM | Candida albicans | |
castanospermine | - |
Candida albicans | |
D-glucose | - |
Candida albicans | |
mannose | - |
Candida albicans | |
additional information | 2-deoxyglucose, 3-deoxyglucose or 6-deoxyglucose do not have any effect on the enzyme activity; the enzyme is completely insensitive to diethyl pyrocarbonate | Candida albicans | |
N-ethylmaleimide | - |
Candida albicans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.133 | - |
4-methylumbelliferyl alpha-D-glucopyranoside | in 10 mM sodium phosphate buffer pH 7.0 and at 37°C | Candida albicans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | - |
Candida albicans | 5783 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47800 | - |
subunit, calculated from amino acid sequence | Candida albicans |
50000 | - |
subunit, SDS-PAGE | Candida albicans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Glc3Man9GlcNAc2 + H2O | Candida albicans | alpha-glucosidase I removes the outermost alpha1,2-glucose unit from the N-linked core Glc3Man9GlcNAc2 | D-glucose + Glc2Man9GlcNAc2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Candida albicans | Q2V621 | - |
- |
Purification (Comment) | Organism |
---|---|
DEAE Bio-Gel A column chromatography and Sephadex G-25 gel filtration | Candida albicans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl alpha-D-glucopyranoside + H2O | not a common substrate for alpha-glucosidase I | Candida albicans | 4-methylumbelliferone + alpha-D-glucopyranose | - |
? | |
Glc3Man9GlcNAc2 + H2O | alpha-glucosidase I removes the outermost alpha1,2-glucose unit from the N-linked core Glc3Man9GlcNAc2 | Candida albicans | D-glucose + Glc2Man9GlcNAc2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alpha-glucosidase I | - |
Candida albicans |
Cwh41p | - |
Candida albicans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 7 | recombinant enzyme, in 10 mM sodium phosphate buffer and at 37°C | Candida albicans |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0027 | - |
in 10 mM sodium phosphate buffer pH 7.0 and at 37°C | Candida albicans | castanospermine | |
0.0029 | - |
in 10 mM sodium phosphate buffer pH 7.0 and at 37°C | Candida albicans | 1-deoxynojirimycin | |
2.2 | - |
in 10 mM sodium phosphate buffer pH 7.0 and at 37°C | Candida albicans | N-ethylmaleimide | |
2.9 | - |
in 10 mM sodium phosphate buffer pH 7.0 and at 37°C | Candida albicans | D-glucose | |
9.1 | - |
in 10 mM sodium phosphate buffer pH 7.0 and at 37°C | Candida albicans | mannose |
General Information | Comment | Organism |
---|---|---|
physiological function | alpha-glucosidase I is required for normal cell wall composition, and virulence of Candida albicans | Candida albicans |