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3.2.1.106: mannosyl-oligosaccharide glucosidase

This is an abbreviated version!
For detailed information about mannosyl-oligosaccharide glucosidase, go to the full flat file.

Word Map on EC 3.2.1.106

Reaction

Glc3Man9GlcNAc2-[protein]
+
H2O
=
Glc2Man9GlcNAc2-[protein]
+
beta-D-glucopyranose

Synonyms

(Glc3)-glucosidase, acid alpha-glucosidase, alpha-glucosidase I, CHO-GAA, CWH41, Cwh41p, GAA, GCSI, Glc I, Glc3 oligosaccharide glucosidase, Glc3-glucosidase, Glc3-oligosaccharide glucosidase, Glc3-OS-glucosidase, Glc3Man9GlcNAc2 oligosaccharide glucosidase, Glc3Man9NAc2 oligosaccharide glucosidase, glucosidase 1, glucosidase I, glucosidase, mannosyloligosaccharide, glucosidase-1, glycoengineered acid alpha-glucosidase, HP-GAA, K12 YgjK protein, KNOPF, mannosyl-oligosaccharide glucosidase, mannosylglycerate hydrolase, MOGS, oxime-neo-rhGAA, PGI, processing alpha-glucosidase I, processing exoglucosidase I, processing glucosidase I, rhGAA, tgGAA, trimming enzyme I, trimming glucosidase I, Tt8MGH, YgjK

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.106 mannosyl-oligosaccharide glucosidase

Storage Stability

Storage Stability on EC 3.2.1.106 - mannosyl-oligosaccharide glucosidase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-18°C, 10% glycerol, few weeks, no loss of activity
-
-20°C, several months, without loss of activity
-
-26°C, lyophilized, stable for at least 1 month
-
-70°C, 200 mM potassium phosphate buffer, pH 6.8, 0.8% Lubrol PX
-
0°C, very slight loss of activity
-
2-4°C, 0.8% Lubrol PX, 200 mM phosphate, pH 6.8, 3 weeks, 50% enzyme activity retained, activity lost rapidly on the removal of detergent
-
25°C, buffer containing 1% poly(ethylene glycol) 4000, 72 h, 75% activity retained
-
27°C, 40 h, more than 50% activity retained
-
37°C, most of activity lost during storage
-
4°C, 1 week, without loss of activity
-
4°C, presence of detergent, 0.8% Lubrol PX, t1/2: 20 days
-
4°C, stable for several weeks
-
storage of the partially purified protein for 1 month at 4°C results in complete loss of the 98000 Da band, but the enzymatic activity remains, suggesting that either one or several of the peptides contain an active catalytic fragment that is resistant to further degradation
-