Information on EC 3.2.1.106 - mannosyl-oligosaccharide glucosidase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.2.1.106
-
RECOMMENDED NAME
GeneOntology No.
mannosyl-oligosaccharide glucosidase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
exohydrolysis of the non-reducing terminal glucose residues in the mannosyl-oligosaccharide Glc3Man9GlcNAc2
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of O-glycosyl bond
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Metabolic pathways
-
-
N-Glycan biosynthesis
-
-
SYSTEMATIC NAME
IUBMB Comments
mannosyl-oligosaccharide glucohydrolase
Also acts, more slowly, on the corresponding glycolipids and glycopeptides. Involved in the formation of high-mannose and complex glycoproteins.
CAS REGISTRY NUMBER
COMMENTARY hide
78413-07-7
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
formerly Aspergillus niger
UniProt
Manually annotated by BRENDA team
formerly Aspergillus niger
UniProt
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain HM1:IMSS
-
-
Manually annotated by BRENDA team
strain HM1:IMSS
-
-
Manually annotated by BRENDA team
strain K12
-
-
Manually annotated by BRENDA team
hen
-
-
Manually annotated by BRENDA team
female C57BL/6
-
-
Manually annotated by BRENDA team
subsp. japonica, cultivar Dongjin
-
-
Manually annotated by BRENDA team
QM474
-
-
Manually annotated by BRENDA team
mung bean
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-methylumbelliferyl alpha-D-glucopyranoside + H2O
4-methylumbelliferone + alpha-D-glucopyranose
show the reaction diagram
not a common substrate for alpha-glucosidase I
-
-
?
4-methylumbelliferyl-alpha-D-glucopyranoside + H2O
4-methylumbelliferone + alpha-D-glucose
show the reaction diagram
-
4°C, pH 6.25
-
-
?
4-methylumbelliferyl-alpha-D-glucoside
4-methylumbelliferone + alpha-D-glucose
show the reaction diagram
-
pH 4.0, 60 min, 37°C
-
-
?
4-methylumbelliferyl-alpha-D-glucoside + H2O
4-methylumbelliferone + D-glucose
show the reaction diagram
4-nitropenyl D-alpha-glucopyranoside + H2O
4-nitrophenol + D-glucose
show the reaction diagram
-
pH 4.3
-
-
?
alpha-D-Glc-(1-2)-alpha-D-Glc-(1-3)-alpha-D-Glc-O(CH2)8-COOCH3 + H2O
beta-D-glucose + alpha-D-Glc-(1-3)-alpha-D-Glc-O(CH2)8-COOCH3
show the reaction diagram
alpha-D-Glc-(1-2)-alpha-D-Glc-(1-3)-alpha-D-Glc-O(CH2)8-COOCH3-tetramethylrhodamine + H2O
beta-D-glucose + alpha-D-Glc-(1-3)-alpha-D-Glc-O(CH2)8-COOCH3-tetramethylrhodamine
show the reaction diagram
cleaves the terminal alpha-1,2-linked glucose
-
-
?
alpha-D-Glc-(1-2)-alpha-D-Glc-(1-3)-alpha-D-Glc-O-(CH2)8COOCH3
?
show the reaction diagram
Glc3Man5GlcNAc + H2O
D-glucose + Glc2Man5GlcNAc
show the reaction diagram
Glc3Man6GlcNAc + H2O
D-glucose + Glc2Man6GlcNAc
show the reaction diagram
Glc3Man7GlcNAc + H2O
D-glucose + Glc2Man7GlcNAc
show the reaction diagram
Glc3Man8GlcNAc + H2O
D-glucose + Glc2Man8GlcNAc
show the reaction diagram
Glc3Man9GlcNAc + H2O
D-glucose + Glc2Man9GlcNAc
show the reaction diagram
Glc3Man9GlcNAc2 + H2O
?
show the reaction diagram
Glc3Man9GlcNAc2 + H2O
D-glucose + Glc2Man9GlcNAc2
show the reaction diagram
Glc3Man9GlcNAc2-lipid + H2O
D-glucose + Glc2Man9GlcNAc2-lipid
show the reaction diagram
Glc3Man9GlcNAc2-peptide + H2O
D-glucose + Glc2Man9GlcNAc2-peptide
show the reaction diagram
Glc3Man9GlcNAc2-pyridylamine + H2O
Glc2Man9GlcNAc2-pyridylamine + D-glucose
show the reaction diagram
Glcalpha(1->2)Glcalpha(1->3)Glcalpha(1->3)Manalpha(1->2)Manalpha(1->2)Manalpha(1->3)Manbeta(1->4)GlcNacbeta(1->4)GlcNacbeta-pyridylamine + H2O
Glcalpha(1->3)Glcalpha(1->3)Manalpha(1->2)Manalpha(1->2)Manalpha(1->3)Manbeta(1->4)GlcNacbeta(1->4)GlcNacbeta-pyridylamine + D-glucose
show the reaction diagram
kojibiose
?
show the reaction diagram
-
pH 5.0, 35°C, 30 min
-
-
?
Maltoheptaose
?
show the reaction diagram
Maltohexaose
?
show the reaction diagram
maltopentaose
?
show the reaction diagram
maltose
?
show the reaction diagram
-
pH 5.0, 35°C, 30 min
-
-
?
Maltotetraose
?
show the reaction diagram
maltotriose
?
show the reaction diagram
nigerose
?
show the reaction diagram
-
pH 5.0, 35°C, 30 min
-
-
?
trehalose
?
show the reaction diagram
-
pH 5.0, 35°C, 30 min
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
Glc3Man9GlcNAc2 + H2O
?
show the reaction diagram
Glc3Man9GlcNAc2 + H2O
D-glucose + Glc2Man9GlcNAc2
show the reaction diagram
Q2V621
alpha-glucosidase I removes the outermost alpha1,2-glucose unit from the N-linked core Glc3Man9GlcNAc2
-
-
?
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phosphate
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1-deoxymannojirimycin
-
very slight inhibition, Ki = 0.19 mM
1-deoxynojirimycin
2,5-Dihydroxymethyl-3,4-dihydroxypyrrolidine
-
plant pyrrolidine alkaloid, DMDP, 0.04 mM: 50% inhibition
2,6-anhydro-1-benzamide-D-glycero-D-ido-heptitol
-
18.2% inhibition at 1 mM
-
4-nitrophenyl-2-deoxy-alpha-D-glucopyranoside
-
56.2% inhibition at 5 mM
4-nitrophenyl-3-deoxy-alpha-D-glucopyranoside
-
71.7% inhibition at 5 mM
4-nitrophenyl-4-deoxy-alpha-D-glucopyranoside
-
18.5% inhibition at 5 mM
4-nitrophenyl-5-deoxy-alpha-D-glucopyranoside
-
22.2% inhibition at 5 mM
4-nitrophenyl-6-deoxy-alpha-D-glucopyranoside
-
32.3% inhibition at 5 mM
Ag+
-
potent inhibitor, inhibition reversed by adding an excess of dithiothreitol
australine
39% inhibition at 0.02 mM
bromoconduritol
22% inhibition at 0.02 mM
castanospermine
chloride
-
inhibitory above 0.3 M, 0.5 M: 30% inhibition
CM-9-78
D-glucose
D-mannose
deoxynojirimycin
-
-
diethyl dicarbonate
-
55% inhibition at 1 mM after 30 min
ethanolamine
-
-
ethyl-3-(3-(dimethylamino)propyl)carbodiimide
-
90% inhibition at 50 mM after 60 min at pH 6.8, 4°C, preincubation with deoxynorjirmycin prevents
glycerol
-
2% or higher: 25% or more inhibition
heavy-metal ions
-
potent inhibitors, inhibition reversed by adding an excess of dithiothreitol
-
Hg2+
-
potent inhibitor, inhibition reversed by adding an excess of dithiothreitol
kojibiose
Man9GlcNAc
-
1 mM thyroglobulin-derived Man9GlcNAc: 22% inhibition
Mg2+
-
slight inhibition
Mn2+
-
slight inhibition
N,N-dimethyl-1-deoxynojirimycin
N-(5-carboxypentyl)-1-deoxynojirimycin
N-(6'-(2'',4''-dinitrophenylamino)hexyl)-1-deoxynojirimycin
-
-
N-(6'-(4''-azido-2''-nitrophenylamino)hexyl)-1-deoxynojirimycin
-
highly potent inhibitor of alpha-glucosidase I
N-5-carboxypentyl-1-deoxymannojirimycin
-
very slight inhibition, Ki = 0.1 mM
N-butyl-1-deoxynojirimycin
-
strong inhibition, Ki = 0.00009 mM
N-butyl-deoxynojirimycin
N-butyldeoxynojirimycin
-
-
N-decanoyl-1-deoxynojirimycin
-
very slight inhibition, Ki = 0.07 mM
N-decyl-1-deoxynojirimycin
-
strong inhibition, Ki = 0.0004 mM
N-dodecyl-1-deoxynojirimycin
-
slight inhibition
N-ethylmaleimide
N-hexyl-1-deoxynojirimycin
-
strong inhibition, Ki = 0.00013 mM
N-methyl-1-deoxymannojirimycin
-
very slight inhibition, Ki = 0.017 mM
N-methyl-1-deoxynojirimycin
N-nonyl-deoxynojirimycin
Nojirimycin
octyl-beta-glucoside
OSL-95II
p-Aminophenyl-beta-thioglucoside
-
100 mM: 73% inhibition
p-nitrophenyl-alpha-D-glucoside
p-nitrophenyl-beta-D-glucoside
PBDNJ0801
PBDNJ0803
PBDNJ0804
phosphate
-
inhibitory above 0.3 M, 0.5 M: 30% inhibition
SDS
-
strong inhibition
sulfate
-
0.1-0.2 M: 15-20% inhibition, somewhate more inhibitory at higher concentrations
tricine
-
-
Tris/maleate
-
10 mM: 60% inhibition
Trypsin
-
up to 40% inhibition in the presence of Lubrol PX, without Lubrol PX: no inhibition
-
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Brij 35
-
at 0.12%: 98% activation
Lubrol Px
NP-40
-
crude enzyme: 3 to 10fold activation
Triton X-100
additional information
-
no significant activation by phospholipids
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.133
4-methylumbelliferyl alpha-D-glucopyranoside
in 10 mM sodium phosphate buffer pH 7.0 and at 37°C
0.036
4-methylumbelliferyl-alpha-D-glucoside
-
-
0.0061
Glc3Man9GlcNAc2-pyridylamine
in 20 mM sodium phosphate, pH 7.0, at 25°C
0.0042
Glcalpha(1->2)Glcalpha(1->3)Glcalpha(1->3)Manalpha(1->2)Manalpha(1->2)Manalpha(1->3)Manbeta(1->4)GlcNacbeta(1->4)GlcNacbeta-pyridylamine
in 20 mM sodium phosphate, pH 7.0, at 25°C
230
nigerose
-
-
additional information
additional information
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1450
Glc3Man9GlcNAc2-pyridylamine
Aspergillus brasiliensis
G1UJA9
in 20 mM sodium phosphate, pH 7.0, at 25°C
1100
Glcalpha(1->2)Glcalpha(1->3)Glcalpha(1->3)Manalpha(1->2)Manalpha(1->2)Manalpha(1->3)Manbeta(1->4)GlcNacbeta(1->4)GlcNacbeta-pyridylamine
Aspergillus brasiliensis
G1UJA9
in 20 mM sodium phosphate, pH 7.0, at 25°C
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.19
1-deoxymannojirimycin
-
very slight inhibition
0.01
1-deoxynojirimycin
-
-
0.04
2,5-Dihydroxymethyl-3,4-dihydroxypyrrolidine
-
plant pyrrolidine alkaloid, DMDP
0.5 - 0.8
kojibiose
0.0005
N,N-dimethyl-1-deoxynojirimycin
-
strong inhibition
0.00045
N-(5-carboxypentyl)-1-deoxynojirimycin
-
strong specific inhibition
0.1
N-5-carboxypentyl-1-deoxymannojirimycin
-
very slight inhibition
0.00009
N-butyl-1-deoxynojirimycin
-
strong inhibition
0.07
N-decanoyl-1-deoxynojirimycin
-
very slight inhibition
0.0004
N-decyl-1-deoxynojirimycin
-
strong inhibition
0.00013
N-hexyl-1-deoxynojirimycin
-
strong inhibition
0.017
N-methyl-1-deoxymannojirimycin
-
very slight inhibition
0.00007
N-methyl-1-deoxynojirimycin
-
-
0.16
Nojirimycin
-
-
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0029 - 0.02
1-deoxynojirimycin
0.0027
castanospermine
Candida albicans
Q2V621
in 10 mM sodium phosphate buffer pH 7.0 and at 37°C
2.9
D-glucose
Candida albicans
Q2V621
in 10 mM sodium phosphate buffer pH 7.0 and at 37°C
0.02
kojibiose
Aspergillus brasiliensis
G1UJA9
in 20 mM sodium phosphate, pH 7.0, at 25°C
9.1
mannose
Candida albicans
Q2V621
in 10 mM sodium phosphate buffer pH 7.0 and at 37°C
0.000108
N-(6'-(2'',4''-dinitrophenylamino)hexyl)-1-deoxynojirimycin
Rattus norvegicus
-
-
0.000017
N-(6'-(4''-azido-2''-nitrophenylamino)hexyl)-1-deoxynojirimycin
Rattus norvegicus
-
-
0.00068
N-butyl-deoxynojirimycin
Rattus norvegicus
-
-
2.2
N-ethylmaleimide
Candida albicans
Q2V621
in 10 mM sodium phosphate buffer pH 7.0 and at 37°C
1.9
nigerose
Aspergillus brasiliensis
G1UJA9
in 20 mM sodium phosphate, pH 7.0, at 25°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2.6
-
soluble activity
4.3
-
isoenzyme CHO-GAA
4.5
-
isoenzyme HP-GAA
5.5
-
membrane-bound activity, with Triton X-100
3000
soluble alpha-glucosidase I
3600
Cwht1p, purified 7.4fold
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 7
recombinant enzyme, in 10 mM sodium phosphate buffer and at 37°C
6.3 - 6.5
-
-
6.5 - 8
-
broad pH optimum between
6.5 - 7.5
-
broad pH optimum between
6.6 - 7
-
-
additional information
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 4.3
-
37°C, 60 min
5.5 - 6.8
-
activity greatly decreases at pH values below 5.5 or above 6.8
5.6 - 7.3
-
half-maximal activity at, steep decrease in activity on acidic side
5.8 - 7.4
-
half-maximal activity
6 - 8
the enzyme retains 89% and 94% of maximum activity at pH 6.0 and 8.0, respectively
6 - 7.5
-
half-maximal activity at
6 - 8.5
-
pH 6.0: about 35% of maximal activity, pH 8.5: about 15% of maximal activity, pH 5.8: no activity
6.2 - 7.2
-
half-maximal activity at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
42
-
pH 5.0
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 4.6
-
CHO-GAA, IEF-Gel
4.2 - 4.6
-
tgGAA, IEF-Gel
4.6 - 4.9
-
HP-GAA, IEF-Gel
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
-
Manually annotated by BRENDA team
-
calf
Manually annotated by BRENDA team
-
flag leaf
Manually annotated by BRENDA team
-
-
Manually annotated by BRENDA team
-
stem base
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE