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3.1.31.1: micrococcal nuclease

This is an abbreviated version!
For detailed information about micrococcal nuclease, go to the full flat file.

Word Map on EC 3.1.31.1

Reaction

endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotide end-products =

Synonyms

AtTSN1, AtTSN2, cTSN, EC 3.1.4.7, Epstein-Barr virusencoded transcription factor 2 co-activator p100, HsTSN, micrococcal DNase, micrococcal endonuclease, Micrococcal nuclease, MN, MN ase, MNase, Nuc, NUC1, Nuc2, nucB, nuclease 8V, nuclease T, nuclease T', nuclease, micrococcal, nuclease, staphylococcal, NucM, P100, PaTSN, PfTSN, ribonucleate (deoxyribo-nucleate) 3'-nucleotidohydrolase, ribonucleate (deoxyribonucleate) 3'-nucleotidohydrolase, S. aureus nuclease, SNA, snake venom phosphodiesterase, SNase, SNAseR, SND1, spleen endonuclease, spleen phosphodiesterase, staph nuclease, staphylococcal nuclease, Staphylococcal nuclease A, Staphylococcal nuclease domain containing-1, staphylococcal nuclease domain-containing 1, staphylococcal nuclease domain-containing protein 1, staphylococcus aureus nuclease, staphylococcus aureus nuclease B, Staphylococcus aureus nuclease homologue, thermonuclease, TNase, TSN, tudor staphylococcal nuclease, Tudor-SN, Tudor-staphylococcal nuclease

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.31 Endoribonucleases that are active with either ribo- or deoxyribonucleic acids and produce 3'-phosphomonoesters
                3.1.31.1 micrococcal nuclease

Cloned

Cloned on EC 3.1.31.1 - micrococcal nuclease

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
a hyperstable, acid-resistant form of SNase known as delta+PHS is expressed in Escherichia coli BL21/DE3 cells
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a plasmid pcDNA-Cap-SNase is constructed for expressing a fusion protein of classical swine fever virus capsid Cap and staphylococcal nuclease, a mammalian cell line PK-15 expressing stably the fusion protein Cap-SNase
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BHL-21 cells stably express staphylococcal nuclease fused to dengue 2 virus capsid protein for CTVI. The intracellular expressed fusion protein is correctly folded and has no cytotoxicity on the mammalian host cells
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cloning of the full-length cDNA from Picea abies encoding Tudor staphylococcal nuclease, expression in Escherichia coli
construction and co-expression of Staphylococcal nuclease in Escherichia coli
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expressed in Escherichia coli
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expression and interaction analysis of the enzyme fused to a GAL4-DNA-binding domain and the three Argonaute proteins in the Saccharomyces cerevisiae strain Y187 two-hybrid system, expression of N-terminally GST-tagged enzyme constructs comprising amino acid residues 1-889, 1-660, and residues 320-889, in Escherichia coli strain Rossetta (DE3)
expression in Escherichia coli
expression in Hep-3B cells
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expression of poly-his-nuclease R in Escherichia coli
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expression of protein fragment results in the formation of insoluble inclusion bodies, successful expression as fusion protein with Maltose Binding Protein
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expression of the enzyme from a plsmid encoding RFP-epitope-tagged Tudor-SN in HeLa cells, localization profile of the Tudor-SN-AGTR1-3'UTR complex, GFP-MS2 fluorescence and immuno-labeling analysis, overview
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expression of wild type and mutant proteins in Escherichia coli BL21(DE3), formation of inclusion bodies
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expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)/pLysS
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gene insert cloned into pET24a+plasmid, transformation of BL21(DE3) Escherichia coli cells for expression
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gene nuc1, recombinant expression in Escherichia coli strain BL21(DE3)
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gene nuc1, sequence comparison and phylogenetic analysis, functional recombinant expression in Escherichia coli strain BL21(DE3)
gene nuc1, sequence comparison, recombinant expression of C-terminally His6-tagged isozyme Nuc1 in Escherichia coli strain strain ER2566
gene nuc2, sequence comparison and phylogenetic analysis, functional recombinant expression in Escherichia coli strain BL21(DE3)
gene nuc2, sequence comparison, the portion of nuc2 encoding amino acids N26-K177 is amplified from AH1263 genomic DNA, recombinant expression of the truncated version as C-terminally His6-tagged isozyme Nuc2, and expression of isozyme Nuc2 as fusion proteins Nuc2-GFP and Nuc2-PhoA, respectively, in Escherichia coli strain strain ER2566, creating strain AH2591. Expression of recombinant chimeric His-tagged fusion proteins in Staphylococcus aureus nuc-deficient mutant strain AH1680
generation of Vibrio anguillarum ghost by coexpression of PhiX 174 Lysis E gene and SNA gene. Gene fragment encoding SNA amplified by PCR using Staphlococcus aureus genomic DNA. construction of plasmid pRK-lambda-P(R)-cI-SNA. Construction of dual vector expressing PhiX 174 lysis E gene and SNA: pRK-kP(R)-cI-E-SNA. Transformation of Escherichia coli SM10-lambda-pir used as donor for plasmid transfer with Vibrio anguillarum via conjugation. Induction of protein expression by temperature elevation
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overexpression of wild-type and mutant enzymes in Escherichia coli
possible target for the anti-malarial therapy tested by RNAi. Role of PfTSN at asexual blood stages investigated by treatment of synchronized parasites at late ring stage with siRNA (analysed by manual counting and by morphological examination 40 h after treatment, additionally analyzed by real time PCR and immunofluoresence), 50 microg/ml concentration of siRNA: over 50% reduction in parasitemia observed, 100 microg/ml concentration of PfTSN siRNA: 60-70% reduction in parasitemia. Effect of PfTSN siRNA over the course of erythrocytic cycle investigated by treatment of parasites with PfTSN siRNA at late ring stage and morphologically examined after the treatment: after 2-3 hours abnormalities (vacuolation, size of vacuols seems to increase over time), after 12 hours most parasites are dead
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proline free mutant
purification of DNA from the cell-associated herpesvirus Marek's disease virus. 150 U of Micrococcal nuclease added to gallid herpesvirus type 2 virus infected cells (GaHV-2 strain 648A) in 100 microl reaction volume, digestion followed by PEG precipitation yields high-molecular weight DNA of greater than 75% pure GaHV-2 DNA suitability for both direct pyrosequencing and further amplification using isothermal polymerase
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recombinant Trp insertion enzymes
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recombinant wild type protein and mutants are expressed in Escherichia coli
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SaeRS is required for expression of the nuc gene, expression from plasmid containing the nuc promoter coupled to sGFP (pCM20), transformed into the Staphylococcus aureus USA300 wild-type-LAC and sae, agr, and sigB mutant strains
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stable enzyme overexpression in hepatocellular carcinoma Hep3B cells
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staphylococcal nuclease fused at its N-terminus to signal peptide of the lactococcal Usp45 protein (SP Usp45-NucB), as reporter for expression and secretion in Lactobacillus bulgaricus, SDS PAGE and western blot of culture supernatant and cell lysate used for analysis
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the enzyme is cloned into an expression-secretion vector lacking its own signal peptide but being fused to a lactococcal sequence encoding a signal peptide. It is then fused to a lactococcal sequence encoding a signal peptide. Functional recombinant expression of the enzyme under the control of a lactococcal promoter, that is inducible by zinc starvation, in Lactococcus lactis subsp. cremoris model strain MG1363, the enzyme is secreted to the GM17v culture medium, expression method optimization, overview
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transient replication of HPV-18 mutant ori plasmids with gene-delivered chimeric mutant AZP-SNase, overexpression of recombinant mutant chimeric enzyme, hybrid nuclease AZP-SNase, in Escherichia coli
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wild type and mutant Snases are expressed in Escherichia coli BL-21 (DE3) as inclusion bodies
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wild type enzyme and the mutants K28C/H124C K28C/K97C are expressed in BL21 (DE3) and K28C/K97C in BL21 (DE3) Escherichia coli cells
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wild-type and S28G mutant expressed in Escherichia coli strain AR120
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