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Literature summary for 3.1.31.1 extracted from
- Characterization and comparative analysis of a second thermonuclease from Staphylococcus aureus (2013), Microbiol. Res., 168, 174-182.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | - |
Staphylococcus aureus |  |
| dithiothreitol | - |
Staphylococcus aureus | |
| DTT | 163.4% of initial isozyme Nuc1 activity at 1 mM | Staphylococcus aureus | |
| Triton X-100 | - |
Staphylococcus aureus | |
| Triton X-100 | 210.5% of initial isozyme Nuc1 activity at 1% | Staphylococcus aureus | |
| Tween-20 | - |
Staphylococcus aureus | |
| Urea | - |
Staphylococcus aureus | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene nuc1, sequence comparison and phylogenetic analysis, functional recombinant expression in Escherichia coli strain BL21(DE3) | Staphylococcus aureus |
| gene nuc2, sequence comparison and phylogenetic analysis, functional recombinant expression in Escherichia coli strain BL21(DE3) | Staphylococcus aureus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | generation of a nuc1/nuc2 double deletion mutant | Staphylococcus aureus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | isozyme Nuc1 shows 79% activity at 5 mM concentration | Staphylococcus aureus | |
| EDTA | - |
Staphylococcus aureus | |
| Mn2+ | isozyme Nuc1 activity drops sharply at 5 mM concentration; isozyme Nuc2 activity decreases with an increase in Mn2+ concentration | Staphylococcus aureus | |
| additional information | Nuc2 shows weaker activity, lower thermostability and different sensitivity to Zn2+ and Mn2+, and in the presence of EDTA or SDS compared with Nuc1, which is consistent with differences in the sequence pattern and structure predicted; Nuc2 shows weaker activity, lower thermostability and different sensitivity to Zn2+ and Mn2+, and in the presence of EDTA or SDS compared with Nuc1, which is consistent with differences in the sequence pattern and structure predicted | Staphylococcus aureus | |
| SDS | - |
Staphylococcus aureus | |
| Zn2+ | isozyme Nuc1 activity drops sharply at 5 mM concentration; isozyme Nuc2 activity decreases with an increase in Zn2+ concentration | Staphylococcus aureus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Staphylococcus aureus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activates to 120% of initial activity at 0.05 mM | Staphylococcus aureus | |
| Co2+ | activates slightly at 5 mM | Staphylococcus aureus | |
| Mg2+ | activates to 120% of initial activity at 0.5 mM | Staphylococcus aureus | |
| additional information | isozyme Nuc1 only exhibits a very small increase in thermonuclease activity in the presence of Ca2+ (0.05 mM), Mg2+ (0.05 mM), Co2+ (0.5 mM) and Ni2+ (0.05 mM) | Staphylococcus aureus | |
| Ni2+ | activates slightly at 0.05 mM | Staphylococcus aureus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Staphylococcus aureus | A0A0H3JPX6 | gene nuc2; strain RN4220, gene nuc2 | - |
| Staphylococcus aureus | Q7A6P2 | gene nuc1; strain RN4220, gene nuc1 | - |
| Staphylococcus aureus N315 | Q7A6P2 | gene nuc1; strain RN4220, gene nuc1 | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | isozyme Nuc1 is active with genomic DNA extracted from Staphylococcus aureus, Listeria monocytogenes, and Salmonella, herring sperm DNA, plasmid DNA pNucc from Staphylococcus aureus and pBR122 from Escherichia coli, and RNA from Staphylococcus aureus, substrate specificity of the recombinant nuclease, overview | Staphylococcus aureus | ? | - |
? | |
| additional information | isozyme Nuc2 is active with genomic DNA extracted from Staphylococcus aureus, Listeria monocytogenes, and Salmonella, herring sperm DNA, plasmid DNA pNucc from Staphylococcus aureus and pBR122 from Escherichia coli, and RNA from Staphylococcus aureus, substrate specificity of the recombinant nuclease, overview | Staphylococcus aureus | ? | - |
? | |
| additional information | isozyme Nuc1 is active with genomic DNA extracted from Staphylococcus aureus, Listeria monocytogenes, and Salmonella, herring sperm DNA, plasmid DNA pNucc from Staphylococcus aureus and pBR122 from Escherichia coli, and RNA from Staphylococcus aureus, substrate specificity of the recombinant nuclease, overview | Staphylococcus aureus N315 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | structure comparison of isozyme Nuc1 and Nuc2, overview | Staphylococcus aureus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NUC1 | - |
Staphylococcus aureus |
| Nuc2 | - |
Staphylococcus aureus |
| staphylococcal nuclease | - |
Staphylococcus aureus |
| thermonuclease | - |
Staphylococcus aureus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
recombinant enzyme | Staphylococcus aureus |
| 55 | - |
- |
Staphylococcus aureus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 80 | effects of temperature on thermonuclease activity of isozymes Nuc1 and Nuc2, profile overview | Staphylococcus aureus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
thermostable enzyme | Staphylococcus aureus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | 10 | - |
Staphylococcus aureus |
| 10 | - |
recombinant enzyme | Staphylococcus aureus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 10 | effects of pH on thermonuclease activity of isozymes Nuc1 and Nuc2, profile overview | Staphylococcus aureus |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | major role for isozyme Nuc1 in terms of thermonuclease activity | Staphylococcus aureus |
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