3.1.31.1: micrococcal nuclease

This is an abbreviated version!
For detailed information about micrococcal nuclease, go to the full flat file.

Reaction

endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotide end-products =

Synonyms

AtTSN1, AtTSN2, cTSN, EC 3.1.4.7, Epstein-Barr virusencoded transcription factor 2 co-activator p100, HsTSN, micrococcal DNase, micrococcal endonuclease, Micrococcal nuclease, MN, MN ase, MNase, Nuc, NUC1, Nuc2, nucB, nuclease 8V, nuclease T, nuclease T', nuclease, micrococcal, nuclease, staphylococcal, NucM, P100, PaTSN, PfTSN, ribonucleate (deoxyribo-nucleate) 3'-nucleotidohydrolase, ribonucleate (deoxyribonucleate) 3'-nucleotidohydrolase, S. aureus nuclease, SNA, snake venom phosphodiesterase, SNase, SNAseR, SND1, spleen endonuclease, spleen phosphodiesterase, staph nuclease, staphylococcal nuclease, Staphylococcal nuclease A, Staphylococcal nuclease domain containing-1, staphylococcal nuclease domain-containing 1, staphylococcal nuclease domain-containing protein 1, staphylococcus aureus nuclease, staphylococcus aureus nuclease B, Staphylococcus aureus nuclease homologue, thermonuclease, TNase, TSN, tudor staphylococcal nuclease, Tudor-SN, Tudor-staphylococcal nuclease

ECTree

     3 Hydrolases

         3.1 Acting on ester bonds

             3.1.31 Endoribonucleases that are active with either ribo- or deoxyribonucleic acids and produce 3'-phosphomonoesters

                3.1.31.1 micrococcal nuclease

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Results	in table
8	Activating Compound
2222	AA Sequence
18	Application
1	CAS Registry Number
38	Cloned(Commentary)
10	Crystallization (Commentary)
486	Disease/ Diagnostics
6	Expression
49	General Information
12	General Stability
1	IC50 Value
23	Inhibitors
8	KM Value [mM]
22	Localization
31	Metals/Ions
10	Molecular Weight [Da]
10	Natural Substrates/ Products (Substrates)
182	Organism
1	Oxidation Stability
328	PDB ID
26	pH Optimum
3	pH Range
6	pH Stability
191	Protein Variants
20	Purification (Commentary)
5	Reaction
9	Reaction Type
122	Reference
5	Renatured (Commentary)
13	Source Tissue
10	Specific Activity [micromol/min/mg]
79	Substrates and Products (Substrate)
17	Subunits
149	Synonyms
13	Temperature Optimum [°C]
1	Temperature Range [°C]
33	Temperature Stability [°C]
1	Turnover Number [1/s]

Purification

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Purification on EC 3.1.31.1 - micrococcal nuclease

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

-

2 entries

10 mM DTT added to expose sulfhydryl groups, and the solution passes through a short column of PD-10 to remove the reducing agent

Staphylococcus sp.

-

696005

proline free mutant

Staphylococcus aureus

P00644

678227

recombinant enzyme

Staphylococcus aureus

-

681430

recombinant enzyme from Lactococcus lactis cell culture medium by cation exchange chromatography

Staphylococcus aureus

-

718081

recombinant fusion protein with Maltose Binding Protein

Plasmodium falciparum

-

680252

recombinant GST-tagged enzyme constructs from Escherichia coli strain Rossetta (DE3) by glutathione affinity chromatography, and anion or cation exchange chromatography, followed by dialysis

Penaeus monodon

G1ARD5

729764

recombinant His-tagged truncated isozyme Nuc2 lacking the N-terminal membrane anchor by nickel-affinity chromatography

Staphylococcus aureus

A0A0H3JPX6, Q7A6P2

730786

recombinant protein

Staphylococcus aureus

-

681601

recombinant protein is purified by anion exchange chromatography on a Q Sepharose Fast Flow column using a linear gradient of NaCl (0-500 mM) in 20 mM Tris-HCl at pH 8.0, 2 mM dithiothreitol and 10% glycerol

3 entries

recombinant proteins

Staphylococcus aureus

-

682777

recombinant wild-type and mutant enzymes from Escherichia coli

2 entries

wild type and mutant enzyme are purified by a series of urea extraction, ethanol precipitation, and ion exchange chromatography

Staphylococcus sp.

-

702709

wild type and mutant enzymes are purified by a chelating-sepharose fast flow column

Staphylococcus sp.

-

705338

-

Staphylococcus aureus

-

135023, 135025, 135030

-

Staphylococcus sp.

-

664892

recombinant protein is purified by anion exchange chromatography on a Q Sepharose Fast Flow column using a linear gradient of NaCl (0-500 mM) in 20 mM Tris-HCl at pH 8.0, 2 mM dithiothreitol and 10% glycerol

Homo sapiens

Q7KZF4

705843

recombinant protein is purified by anion exchange chromatography on a Q Sepharose Fast Flow column using a linear gradient of NaCl (0-500 mM) in 20 mM Tris-HCl at pH 8.0, 2 mM dithiothreitol and 10% glycerol

Picea abies

Q0JRI3

705843

recombinant protein is purified by anion exchange chromatography on a Q Sepharose Fast Flow column using a linear gradient of NaCl (0-500 mM) in 20 mM Tris-HCl at pH 8.0, 2 mM dithiothreitol and 10% glycerol

Arabidopsis thaliana

Q8VZG7, Q9FLT0

705843

recombinant wild-type and mutant enzymes from Escherichia coli

Staphylococcus aureus

-

730910

recombinant wild-type and mutant enzymes from Escherichia coli

Staphylococcus sp.

-

718378