3.1.31.1: micrococcal nuclease

This is an abbreviated version!
For detailed information about micrococcal nuclease, go to the full flat file.

Reaction

endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotide end-products =

Synonyms

AtTSN1, AtTSN2, cTSN, EC 3.1.4.7, Epstein-Barr virusencoded transcription factor 2 co-activator p100, HsTSN, micrococcal DNase, micrococcal endonuclease, Micrococcal nuclease, MN, MN ase, MNase, Nuc, NUC1, Nuc2, nucB, nuclease 8V, nuclease T, nuclease T', nuclease, micrococcal, nuclease, staphylococcal, NucM, P100, PaTSN, PfTSN, ribonucleate (deoxyribo-nucleate) 3'-nucleotidohydrolase, ribonucleate (deoxyribonucleate) 3'-nucleotidohydrolase, S. aureus nuclease, SNA, snake venom phosphodiesterase, SNase, SNAseR, SND1, spleen endonuclease, spleen phosphodiesterase, staph nuclease, staphylococcal nuclease, Staphylococcal nuclease A, Staphylococcal nuclease domain containing-1, staphylococcal nuclease domain-containing 1, staphylococcal nuclease domain-containing protein 1, staphylococcus aureus nuclease, staphylococcus aureus nuclease B, Staphylococcus aureus nuclease homologue, thermonuclease, TNase, TSN, tudor staphylococcal nuclease, Tudor-SN, Tudor-staphylococcal nuclease

ECTree

     3 Hydrolases

         3.1 Acting on ester bonds

             3.1.31 Endoribonucleases that are active with either ribo- or deoxyribonucleic acids and produce 3'-phosphomonoesters

                3.1.31.1 micrococcal nuclease

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Results	in table
8	Activating Compound
2222	AA Sequence
18	Application
1	CAS Registry Number
38	Cloned(Commentary)
10	Crystallization (Commentary)
486	Disease/ Diagnostics
6	Expression
49	General Information
12	General Stability
1	IC50 Value
23	Inhibitors
8	KM Value [mM]
22	Localization
31	Metals/Ions
10	Molecular Weight [Da]
10	Natural Substrates/ Products (Substrates)
182	Organism
1	Oxidation Stability
328	PDB ID
26	pH Optimum
3	pH Range
6	pH Stability
191	Protein Variants
20	Purification (Commentary)
5	Reaction
9	Reaction Type
122	Reference
5	Renatured (Commentary)
13	Source Tissue
10	Specific Activity [micromol/min/mg]
79	Substrates and Products (Substrate)
17	Subunits
149	Synonyms
13	Temperature Optimum [°C]
1	Temperature Range [°C]
33	Temperature Stability [°C]
1	Turnover Number [1/s]

pH Stability

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pH Stability on EC 3.1.31.1 - micrococcal nuclease

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pH STABILITY

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

3.5 - 9

Staphylococcus sp.

-

measurement of stability by two methods: first fluorescence-monitored denaturation with urea, hydrochloric acid and heat. Second by numerical integration of acid titration curves measured potentiometrically under native and unfolding conditions

657910

4.15

Staphylococcus sp.

-

pH-value, at which the acid-denaturation is half completed in wild-type enzyme

664352

4.5 - 9

Staphylococcus sp.

-

25°C, wild-type enzyme is stable in the range

666025

4.8

Staphylococcus sp.

-

25°C, maximal stability of mutant enzyme I92E

666025

5 - 10

Staphylococcus aureus

-

recombinant mutant enzymes, stable, overview

729399

9.8

Staphylococcus sp.

-

25°C, maximal stability of mutant enzyme I92K

666025