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Literature summary for 3.1.31.1 extracted from

  • Onitsuka, M.; Kamikubo, H.; Yamazaki, Y.; Kataoka, M.
    Mechanism of induced folding: Both folding before binding and binding before folding can be realized in staphylococcal nuclease mutants (2008), Proteins, 72, 837-847.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild type and mutant proteins in Escherichia coli BL21(DE3), formation of inclusion bodies Staphylococcus aureus

Protein Variants

Protein Variants Comment Organism
DELTA140-149 deletion of the 10 C-terminal residues, mutant proteins are in a non-native or disordered state under physiological conditions, folding is induced by addition of an inhibitor or substrate Staphylococcus aureus
INS33A34 insertion of an alanine between residues 33 and 34, mutant proteins are in a non-native or disordered state under physiological conditions, folding is induced by addition of an inhibitor or substrate Staphylococcus aureus

Inhibitors

Inhibitors Comment Organism Structure
adenosine 3',5'-diphosphate able to induce folding of mutant proteins into the native state Staphylococcus aureus

Organism

Organism UniProt Comment Textmining
Staphylococcus aureus
-
native state protein and engineered proteins showing a disordered state under physiological conditions
-

Purification (Commentary)

Purification (Comment) Organism
recombinant proteins Staphylococcus aureus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ssDNA + H2O single stranded salmon sperm DNA Staphylococcus aureus ?
-
?

Synonyms

Synonyms Comment Organism
SNase
-
Staphylococcus aureus
staphylococcal nuclease
-
Staphylococcus aureus