Cloned (Comment) | Organism |
---|---|
expression of wild type and mutant proteins in Escherichia coli BL21(DE3), formation of inclusion bodies | Staphylococcus aureus |
Protein Variants | Comment | Organism |
---|---|---|
DELTA140-149 | deletion of the 10 C-terminal residues, mutant proteins are in a non-native or disordered state under physiological conditions, folding is induced by addition of an inhibitor or substrate | Staphylococcus aureus |
INS33A34 | insertion of an alanine between residues 33 and 34, mutant proteins are in a non-native or disordered state under physiological conditions, folding is induced by addition of an inhibitor or substrate | Staphylococcus aureus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
adenosine 3',5'-diphosphate | able to induce folding of mutant proteins into the native state | Staphylococcus aureus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | - |
native state protein and engineered proteins showing a disordered state under physiological conditions | - |
Purification (Comment) | Organism |
---|---|
recombinant proteins | Staphylococcus aureus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ssDNA + H2O | single stranded salmon sperm DNA | Staphylococcus aureus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SNase | - |
Staphylococcus aureus |
staphylococcal nuclease | - |
Staphylococcus aureus |