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Literature summary for 3.1.31.1 extracted from
- Conformational consequences of ionization of Lys, Asp and Glu buried at position 66 in staphylococcal nuclease (2010), Biochemistry, 49, 4138-4146.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant wild type protein and mutants are expressed in Escherichia coli | Staphylococcus sp. |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| the V66K/P117G/H124L/S128A variant of nuclease is crystallized by the hanging drop vapor diffusion method at 4°C, 2 data sets are collected at -173.15°C, at pH 7 and 4.7, and the third is collected at 25.15°C and pH 5 | Staphylococcus sp. |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| V66D/P117G/H124L/S128A | production by site-directed mutagenesis, pka value shifts to 7.79 and, after chemical denaturation, to 8.05 | Staphylococcus sp. |
| V66D/P117G/H124L/S128A/G50F/V51N/DELTA44-49 | production by site-directed mutagenesis, pka value shifts to 8.95 and, after chemical denaturation, to 8.73 | Staphylococcus sp. |
| V66E/P117G/H124L/S128A | production by site-directed mutagenesis, pka value shifts to 8.80 and, after chemical denaturation, to 8.99 | Staphylococcus sp. |
| V66E/P117G/H124L/S128A/G50F/V51N/DELTA44-49 | production by site-directed mutagenesis, pka value shifts to 9.07 and, after chemical denaturation, to 8.80 | Staphylococcus sp. |
| V66K | production by site-directed mutagenesis, pka value shifts to 6.38 after chemical denaturation | Staphylococcus sp. |
| V66K/P117G/H124L/S128A | production by site-directed mutagenesis, pka value shifts to 6.35 | Staphylococcus sp. |
| V66K/P117G/H124L/S128A/G50F/V51N/DELTA44-49 | production by site-directed mutagenesis, pka value shifts to 5.63 and, after chemical denaturation, to 5.83 | Staphylococcus sp. |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Staphylococcus sp. | - |
- |
- |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| SNase | - |
Staphylococcus sp. |
| staphylococcal nuclease | - |
Staphylococcus sp. |
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