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evolution
sequence comparison and phylogenetic analysis, Staphylococcus clustering, overview. The divergent Staphylococcus aureus clade harbors a homologue of the thermostable nuclease (NucM) whose nucleotide sequence is highly divergent from those of nuc1 and nuc2 of Staphylococcus aureus reference strains
evolution
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sequence comparison and phylogenetic analysis, Staphylococcus clustering, overview. The divergent Staphylococcus aureus clade harbors a homologue of the thermostable nuclease (NucM) whose nucleotide sequence is highly divergent from those of nuc1 and nuc2 of Staphylococcus aureus reference strains
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evolution
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sequence comparison and phylogenetic analysis, Staphylococcus clustering, overview. The divergent Staphylococcus aureus clade harbors a homologue of the thermostable nuclease (NucM) whose nucleotide sequence is highly divergent from those of nuc1 and nuc2 of Staphylococcus aureus reference strains
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evolution
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sequence comparison and phylogenetic analysis, Staphylococcus clustering, overview. The divergent Staphylococcus aureus clade harbors a homologue of the thermostable nuclease (NucM) whose nucleotide sequence is highly divergent from those of nuc1 and nuc2 of Staphylococcus aureus reference strains
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malfunction
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when the nuc1 gene is knocked out, the ability of Staphylococcus aureus strains to form a biofilm significantly increased
malfunction
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conditioned medium from Hep3B-SND1 cells stably overexpressing SND1 augmentes, whereas that from QGYSND1si cells stably overexpressing SND1 siRNA significantly inhibits angiogenesis, as analyzed by a chicken chorioallantoic membrane assay and a human umbilical vein endothelial cell differentiation assay
malfunction
mutations of either or both of the nuclease genes nuc1 and nuc2 result in an enhanced capacity to form a biofilm, mutation of nuc2 also has an impact on the ability of a sarA mutant to form a biofilm, at least in the absence of coating with plasma proteins. The susceptibility to daptomycin is reduced in the mutants
malfunction
mutations of either or both of the nuclease genes nuc1 and nuc2 result in an enhanced capacity to form a biofilm. The susceptibility to daptomycin is reduced in the mutants
malfunction
suppression of Penaeus monodon Tudor staphylococcal nuclease by double-stranded RNA results in decreasing dsRNA-mediated gene silencing activity. Knockdown of Argonaute protein PmAgo1 and the enzyme diminishes the ability of dsRNA-Rab7 to knockdown PmRab7 expression
malfunction
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mutations of either or both of the nuclease genes nuc1 and nuc2 result in an enhanced capacity to form a biofilm. The susceptibility to daptomycin is reduced in the mutants
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malfunction
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when the nuc1 gene is knocked out, the ability of Staphylococcus aureus strains to form a biofilm significantly increased
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metabolism
programmed cell death pathways, Tudor staphylococcal nuclease is a new component of the human programmed degradome and is cleaved by caspase-3 between the Tudor and SN5 domains
metabolism
programmed cell death, enzyme is a part of the stress-induced cell-death degradome during both developmental and stress-induced cell deaths
metabolism
extracellular DNA promotes biofilm formation in Staphylococcus aureus and, conversely, extracellular nucleases limit the ability to form a biofilm
metabolism
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inhibition of NF-kappaB blocks enzyme-induced angiogenesis
metabolism
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extracellular DNA promotes biofilm formation in Staphylococcus aureus and, conversely, extracellular nucleases limit the ability to form a biofilm
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physiological function
ribonuclease activity, control of gene expression by activating transcription, subsequent mRNA splicing, regulation of RNA silencing and in the pathway that edits and destroys double-stranded RNA, uncleavable Tudor staphylococcal nuclease stimulates cell proliferation and protects cells from death
physiological function
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Staphylococcal nuclease degrades both DNA and RNA
physiological function
staphylococcal nuclease domain-containing protein 1 is a component of the RNA-induced splicing complex that mediates RNA interference, leading to degradation of specific mRNAs
physiological function
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presence and ionization of Lys66, buried in the hydrophobic core of a stabilized variant of staphylococcal nuclease, affect conformation and dynamics. The neutral Lys66 affects slow conformational fluctuations globally, whereas the effects of the charged form are localized to the region immediately surrounding position 66, when Lys66 is charged the protein expands, structural reorganization triggered by ionization of the internal Lys66, detailed overview
physiological function
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staphylococcal nuclease is an important virulence factor of Staphylococcus aureus. Biofilm development can be prevented in staphylococcal nuclease-producing strains of Staphylococcus aureus, direct relationship between staphylococcal nuclease production and the prevention of biofilm development, overview. Staphylococcal nuclease affects biofilm formation by other bacteria, such as Pseudomonas aeruginosa and Staphylococcus epidermis. Only bacterial strains that do not possess the nuc1 gene are able to form biofilms
physiological function
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Staphylococcus aureus nuclease mediates resistance against entrapment and killing within neutrophil extracellular traps, which consist of a nuclear DNA backbone associated with antimicrobial peptides, histones and proteases that provide a matrix to entrap and kill various microbes. Promoting role of Staphylococcus aureus nuclease in neutrophil extracellular traps degradation and virulence in a murine respiratory tract infection model, overview. Staphylococcus aureus nuclease facilitates evasion from neutrophil extracellular trap entrapment, and mediates resistance against extracellular killing by neutrophils
physiological function
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enzyme hepatocellular carcinoma Staphylococcal nuclease domain containing-1 promotes tumorigenesis in human hepatocellular carcinoma cells. It increases angiotensin II type 1 receptor (AT1R) levels by increasing AT1R mRNA stability. That results in activation of ERK, Smad2 and subsequently the TGFbeta signaling pathway, promoting epithelial-mesenchymal transition and migration and invasion by human hepatocellular carcinoma cells. The enzyme modulates the TGFbeta signaling pathway
physiological function
extracellular nucleases limit the ability to form a biofilm. The role of extracellular nucleases under in vitro versus in vivo conditions differ
physiological function
involvement of PmAgo1 and the enzyme in shrimp RNAi pathway, RNAi-based mechanism in shrimp, overview
physiological function
isozyme Nuc2 is detrimental to Staphylococcus aureus biofilms, purified isozyme Nuc2 prevents biofilm formation in vitro and modestly decreases biomass in dispersal experiments
physiological function
major role for isozyme Nuc1 in terms of thermonuclease activity
physiological function
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the enzyme is a stress-related protein, enzyme Tudor-SN plays an important role in the assembly of AGTR1-3' UTR granules affecting the recovery kinetics of stress granules
physiological function
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the enzyme staphylococcal nuclease domain-containing 1, SND1, regulates a variety of cellular functions and promotes an aggressive tumorigenic phenotype in hepatocellular carcinoma cells. Role of SND1 in regulating tumor angiogenesis, a hallmark of cancer, overview. SND1 regulates NF-kappaB and miR-221, two important determinants of hepatocellular carcinoma controlling the aggressive phenotype. The enzyme induces angiogenesis by up-regulating angiogenin and CXCL16. The phosphorylation of IKKalpha and IkappaBalpha upon enzyme overexpression suggests activation of a canonical NF-kappa B signaling pathway by the enzyme
physiological function
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the MNase digestion of nucleosomes assembled on a strong nucleosome positioning sequence, Widom's clone 601, releases nucleosome cores whose sizes are strongly affected by the linker DNA sequence
physiological function
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the nuc gene necoding Staphylococcus aureus nuclease is under the control of the SaeRS two-component system, which is a major regulator of Staphylococcus aureus virulence determinants, the enzyme is an SaeRS-dependent virulence factor. With community-associated methicillin-resistant Staphylococcus aureus in a mouse model of peritonitis, in vivo expression of Nuc activity in an SaeRS-dependent manner is observed and determination of Nuc as a virulence factor that is important for in vivo survival, confirming the enzyme's role as a contributor to invasive disease. The enzyme contributes to pathogen survival during invasive disease
physiological function
the downregulated expression of Tudor-staphylococcal nuclease can decrease cancer malignancy, and the overexpression can increase viability and migration potential of various tumor cell types. Tudor-SN silencing suppresses the expression of alkylglycerone phosphate synthase (AGPS) and the activity of the mechanistic target of rapamycin (mTOR) signaling pathway. NF-kappaB and miR-127 may be the mediators of Tudor-SN-regulated alkylglycerone phosphate synthase (AGPS) via the mTOR signaling pathway
physiological function
the enzyme cooperates with RNA editing to eliminate duplex RNA in cell defense. Tudor staphylococcal nuclease selects and degrades RNA during microRNA decay
physiological function
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isozyme Nuc2 is detrimental to Staphylococcus aureus biofilms, purified isozyme Nuc2 prevents biofilm formation in vitro and modestly decreases biomass in dispersal experiments
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physiological function
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extracellular nucleases limit the ability to form a biofilm. The role of extracellular nucleases under in vitro versus in vivo conditions differ
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physiological function
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major role for isozyme Nuc1 in terms of thermonuclease activity
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physiological function
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Staphylococcus aureus nuclease mediates resistance against entrapment and killing within neutrophil extracellular traps, which consist of a nuclear DNA backbone associated with antimicrobial peptides, histones and proteases that provide a matrix to entrap and kill various microbes. Promoting role of Staphylococcus aureus nuclease in neutrophil extracellular traps degradation and virulence in a murine respiratory tract infection model, overview. Staphylococcus aureus nuclease facilitates evasion from neutrophil extracellular trap entrapment, and mediates resistance against extracellular killing by neutrophils
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physiological function
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staphylococcal nuclease is an important virulence factor of Staphylococcus aureus. Biofilm development can be prevented in staphylococcal nuclease-producing strains of Staphylococcus aureus, direct relationship between staphylococcal nuclease production and the prevention of biofilm development, overview. Staphylococcal nuclease affects biofilm formation by other bacteria, such as Pseudomonas aeruginosa and Staphylococcus epidermis. Only bacterial strains that do not possess the nuc1 gene are able to form biofilms
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additional information
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MNase mapping of the enhancer chromatin structure in the Drosophila melanogaster embryo to analyse chromatin structure in a developmental setting, and identification of structural changes on a cis-regulatory element targeted by the Knirps repressor, method development and optimization, overview
additional information
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residue W140 is critical to SNase structure and function
additional information
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thermodynamic parameters derived from urea-induced unfolding of mutant SNase140 and mutant SNase141 in comparison with those of wild-type SNase and mutant SNase140 in the presence of calcium, backbone dynamics, detailed overview. Mutant SNase140 unfolds easily compared to mutant SNase141 and wild-type SNase
additional information
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enzyme tryptophan fluorescence spectra, fluorescence measurements of protein unfolding under pressure, high-pressure fluorescence spectroscopy and single value decomposition analysis, overview
additional information
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mannosylglycerate preferentially affects specific structural elements of the P117G/H124L/S128A mutant enzyme, structure analysis, overview
additional information
sequence and structure comparisons of isozymes Nuc1 and Nuc2, overview
additional information
sequence and structure comparisons of isozymes Nuc1 and Nuc2, overview
additional information
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staphylococcal nuclease domain-containing 1, SND1, is a multifunctional nuclease containing four staphylococcal nuclease domains and a tudor domain. No potential interaction between enzyme SND1 and p65 subunit of NF-kappa B
additional information
the enzyme interacts with Argonaute protein PmAgo1, but not with PmAgo2 or PmAgo3. Interaction between PmAgo and the enzyme is mediated through the N-terminal domain of PmAgo1 and the SN1-2 domains of the enzyme, interaction analysis and mapping using the two-hybrid system for different protein constructs, overview
additional information
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the enzyme interacts with Argonaute protein PmAgo1, but not with PmAgo2 or PmAgo3. Interaction between PmAgo and the enzyme is mediated through the N-terminal domain of PmAgo1 and the SN1-2 domains of the enzyme, interaction analysis and mapping using the two-hybrid system for different protein constructs, overview
additional information
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the fluorescence detected guanidine hydrochloride equilibrium denaturation of wild-type staphylococcal nuclease does not fit a three-state unfolding model, overview. Method evaluation to distinguish a two-state from a three-state denaturation
additional information
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the introduction of internal cavities into different subdomains affects local stability, flexibility, and dynamics of the enzyme, NMR spectroscopy under atmospheric and high pressure, H/D exchange and molecular dynamics simulations of wild-type and mutant enzymes, overview. Responses to the creation of cavities cannot be anticipated from global thermodynamic stability or crystal structures, they depend on the local structural and energetic context of the substitutions
additional information
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sequence and structure comparisons of isozymes Nuc1 and Nuc2, overview
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