Cloned (Comment) | Organism |
---|---|
cloning of the full-length cDNA from Picea abies encoding Tudor staphylococcal nuclease, expression in Escherichia coli | Picea abies |
expression in Escherichia coli | Homo sapiens |
expression in Escherichia coli | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
D790E | by site-directed mutagenesis, proteolysis of Tudor staphylococcal nuclease does not occur either when the P1 position of the DAVD motif is mutated or after treatment with the pan-caspase inhibitor zVAD-fluoromethylketone, expression of mutant under normal conditions enhances cell proliferation in both cancer (HeLa) and non-cancer (HEK-293) cells compared with mock- and wild-type Tudor staphylococcal nuclease-transfected samples, under camptothecin-induced apoptosis, expression of Tudor staphylococcal nuclease mutant results in a 35% increment in viable HeLa cells, suggesting that caspase-mediated proteolysis of enzyme is important for the progression of apoptosis | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
caspase-3 | Tudor staphylococcal nuclease, a multifunctional regulator of gene expression, is cleaved by caspase-3 during apoptosis, this cleavage impairs the ability of Tudor staphylococcal nuclease to activate mRNA splicing, inhibits its ribonuclease activity and is important for the execution of apoptosis, cleavage of enzyme lowers its nuclease activity by almost 50% | Homo sapiens | |
deoxythymidine 3',5'-bisphosphate | nuclease activity of enzyme is highly sensitive to deoxythymidine 3',5'-bisphosphate, a specific inhibitor of staphylococcal nuclease, whereas the inactive analogue deoxythymidine 3'-phosphate has no inhibitory effect | Homo sapiens | |
deoxythymidine 3',5'-bisphosphate | nuclease activity of PaTSN is highly sensitive to deoxythymidine 3',5'-bisphosphate, a specific inhibitor of staphylococcal nuclease, whereas the inactive analogue deoxythymidine 3'-phosphate has no inhibitory effect | Picea abies | |
metacaspase mcII-Pa | metacaspase mcII-Pa cleaves the phylogenetically conserved protein Tudor staphylococcal nuclease during both developmental and stress-induced programmed cell death | Homo sapiens | |
metacaspase mcII-Pa | metacaspase mcII-Pa processes recombinant enzyme into 5 major fragments, inactivation of mcII-Pa either by the inhibitor EGR-chloromethyl ketone or through mutation of catalytic Cys139 blocked Tudor staphylococcal nuclease fragmentation, proteolysis of endogenous enzyme correlates with metacaspase activity, reaching the maximum level in early embryos and declining to non-detectable levels in mature embryos, cleavage of enzyme by mcII-Pa leads to a 90% decrease in ribonuclease activity, whereas inactivation of mcII-Pa by either EGR-cmk or mutation of catalytic Cys139 left TSN intact and fully active | Picea abies | |
additional information | induction of apoptosis in HCT116 colon carcinoma cells with 5-fluorouracil leads to increased caspase-3-like activity and cleavage of endogenous Tudor staphylococcal nuclease into 2 fragments with the same molecular mass in vitro, this effect is not cell- and drug-specific because a similar pattern of endogenous Tudor staphylococcal nuclease cleavage is detected in camptothecin-treated HeLa cells | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | in the interphase and mitosis | Arabidopsis thaliana | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 30 mM CaCl2 are included in assay medium | Picea abies | |
Ca2+ | 5 mM CaCl2 are included in assay medium | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q8VZG7 | - |
- |
Arabidopsis thaliana | Q9FLT0 | - |
- |
Homo sapiens | Q7KZF4 | - |
- |
Picea abies | Q0JRI3 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein is purified by anion exchange chromatography on a Q Sepharose Fast Flow column using a linear gradient of NaCl (0-500 mM) in 20 mM Tris-HCl at pH 8.0, 2 mM dithiothreitol and 10% glycerol | Homo sapiens |
recombinant protein is purified by anion exchange chromatography on a Q Sepharose Fast Flow column using a linear gradient of NaCl (0-500 mM) in 20 mM Tris-HCl at pH 8.0, 2 mM dithiothreitol and 10% glycerol | Picea abies |
recombinant protein is purified by anion exchange chromatography on a Q Sepharose Fast Flow column using a linear gradient of NaCl (0-500 mM) in 20 mM Tris-HCl at pH 8.0, 2 mM dithiothreitol and 10% glycerol | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
embryo | - |
Picea abies | - |
embryogenic cell line | - |
Picea abies | - |
Synonyms | Comment | Organism |
---|---|---|
AtTSN1 | - |
Arabidopsis thaliana |
AtTSN2 | - |
Arabidopsis thaliana |
HsTSN | - |
Homo sapiens |
P100 | - |
Homo sapiens |
PaTSN | - |
Picea abies |
SND1 | - |
Homo sapiens |
TSN | - |
Homo sapiens |
TSN | - |
Picea abies |
tudor staphylococcal nuclease | - |
Homo sapiens |
tudor staphylococcal nuclease | - |
Picea abies |
tudor staphylococcal nuclease | - |
Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Homo sapiens |
7 | - |
assay at | Picea abies |
Organism | Comment | Expression |
---|---|---|
Arabidopsis thaliana | down-regulation of enzyme using interfering RNA results in pollen degeneration and a dramatic reduction in plant fertility, providing further evidence for an essential role of TSN in plant reproduction | down |
Homo sapiens | Tudor staphylococcal nuclease knockdown leads to activation of ectopic cell death during reproduction, impairing plant fertility, HeLa cells transfected with Tudor staphylococcal nuclease short interfering RNA show a dramatic increase in apoptotic response to camptothecin accompanied by a 7.9fold increase in activation of caspase-3 and increased cleavage of PARP and lamin-A (by 3.7fold and 6.7fold, respectively), moreover, reduction of Tudor staphylococcal nuclease levels induces apoptosis even in the absence camptothecin, leading to 7.7fold, 6.1fold and 11fold increases in apoptotic markers, demonstrating that Tudor staphylococcal nuclease is indispensable for the maintenance of cell viability | additional information |
General Information | Comment | Organism |
---|---|---|
metabolism | programmed cell death pathways, Tudor staphylococcal nuclease is a new component of the human programmed degradome and is cleaved by caspase-3 between the Tudor and SN5 domains | Homo sapiens |
metabolism | programmed cell death, enzyme is a part of the stress-induced cell-death degradome during both developmental and stress-induced cell deaths | Picea abies |
physiological function | ribonuclease activity, control of gene expression by activating transcription, subsequent mRNA splicing, regulation of RNA silencing and in the pathway that edits and destroys double-stranded RNA, uncleavable Tudor staphylococcal nuclease stimulates cell proliferation and protects cells from death | Homo sapiens |