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1.18.6.1: nitrogenase

This is an abbreviated version!
For detailed information about nitrogenase, go to the full flat file.

Word Map on EC 1.18.6.1

Reaction

4 reduced ferredoxin + 8 H+ +

N2
+ 16 ATP + 16 H2O = 4 oxidized ferredoxin +
H2
 + 2 NH3 + 16 ADP + 16 phosphate

Synonyms

ARA, dinitrogenase, EC 1.18.2.1, Kp2, Mo nitrogenase, Mo-dependent nitrogenase, Mo-Fe nitrogenase, Mo-nitrogenase, MoFe protein, MoFe-protein, molybdenum nitrogenase, molybdenum-containing nitrogenase, molybdenum-iron protein, molybdenum-nitrogenase, More, N2ase, nif, nif1, nif2, nifD, NifDK, NifH, NifH2, nifHDK, nifK, nifM, nitrogenase Fe protein, nitrogenase Fe-protein, nitrogenase FeVco, nitrogenase iron-protein, nitrogenase MoFe protein, nitrogenase MoFe-protein, nitrogenase molybdenum iron protein, nitrogenase molybdenum-iron protein, V-nitrogenase, vanadium nitrogenase, vanadium-nitrogenase, VnfDGK, VnfH

ECTree

     1 Oxidoreductases
         1.18 Acting on iron-sulfur proteins as donors
             1.18.6 With dinitrogen as acceptor
                1.18.6.1 nitrogenase

Purification

Purification on EC 1.18.6.1 - nitrogenase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
2 forms of VFe protein
-
all components
-
both components
FeMo-cofactorless MoFe protein from nifB deletion mutant
-
iron-only nitrogenase, both components
-
large scale
molybdenum containing enzyme form, both components
-
native NifDK by gel filtration and affinity chromatography on a NifB resin, elution as NifDK/NifB-co complex
-
partially, extraction of FeVco in an active form by N-methylformamide, and gel filtration of the reconstituted protein, overview
-
purification of the enzyme complex and isolation of the MoFe protein thereof
-
recombinant Azotobacter vinelandii NafY protein from Escherichia coli strain BL21(DE3)
-
recombinant His-tagged NifH2 from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography, ultrafiltration, and gel filtration to over 95% purity
-
recombinant wild-type Fe protein, recombinant His-tagged wild-type MoFe protein and recombinant His-tagged MoFe protein mutant V70A, to homogeneity
-
strict anaerobic conditions
wild-type and mutant H195Q
-
wild-type and mutants H195Q, H195N, Q191K
-
wild-type and tungsten-substituted enzymes
-