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Literature summary for 1.18.6.1 extracted from

  • Song, S.D.; Hartmann, A.; Burris, R.H.
    Purification and properties of the nitrogenase of Azospirillum amazonense (1985), J. Bacteriol., 164, 1271-1277.
    View publication on PubMedView publication on EuropePMC

Metals/Ions

Metals/Ions Comment Organism Structure
Iron 24 atoms of iron per molecule of MoFe protein Nitrospirillum amazonense
Molybdenum 2 gatom per mol of MoFe protein Nitrospirillum amazonense

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
-
Nitrospirillum amazonense

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O Nitrospirillum amazonense
-
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
?
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O Nitrospirillum amazonense Y1
-
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
?

Organism

Organism UniProt Comment Textmining
Nitrospirillum amazonense
-
-
-
Nitrospirillum amazonense Y1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
strain Y1 Nitrospirillum amazonense

Reaction

Reaction Comment Organism Reaction ID
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein Nitrospirillum amazonense

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8
-
purified Fe protein Nitrospirillum amazonense
2.4
-
purified MoFe protein Nitrospirillum amazonense

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
Nitrospirillum amazonense 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
?
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
Nitrospirillum amazonense Y1 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
?
dithionite + H+ + acetylene + ATP
-
Nitrospirillum amazonense ?
-
?
dithionite + H+ + acetylene + ATP
-
Nitrospirillum amazonense Y1 ?
-
?

Subunits

Subunits Comment Organism
More
-
Nitrospirillum amazonense

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Nitrospirillum amazonense