Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | Fe protein contains an adenine-like molecule, a pentose moiety and a phosphate residue covalently attached to the molecule | Rhodobacter capsulatus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | - |
Rhodobacter capsulatus | |
Molybdenum | - |
Rhodobacter capsulatus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Rhodobacter capsulatus |
33500 | - |
MoFe protein 1 * 55000 + 1 * 59000 + Fe protein 2 * 33500, SDS-PAGE | Rhodobacter capsulatus |
55000 | - |
MoFe protein 1 * 55000 + 1 * 59000 + Fe protein 2 * 33500, SDS-PAGE | Rhodobacter capsulatus |
59000 | - |
MoFe protein 1 * 55000 + 1 * 59000 + Fe protein 2 * 33500, SDS-PAGE | Rhodobacter capsulatus |
63000 | - |
Fe protein | Rhodobacter capsulatus |
230000 | - |
MoFe protein | Rhodobacter capsulatus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O | Rhodobacter capsulatus | - |
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodobacter capsulatus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rhodobacter capsulatus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate | enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein | Rhodobacter capsulatus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.6 | 0.8 | purified Fe protein | Rhodobacter capsulatus |
1.5 | 1.7 | purified MoFe protein | Rhodobacter capsulatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O | - |
Rhodobacter capsulatus | 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | - |
Rhodobacter capsulatus |
tetramer | MoFe protein 1 * 55000 + 1 * 59000 + Fe protein 2 * 33500, SDS-PAGE | Rhodobacter capsulatus |