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1.18.6.1: nitrogenase

This is an abbreviated version!
For detailed information about nitrogenase, go to the full flat file.

Word Map on EC 1.18.6.1

Reaction

4 reduced ferredoxin + 8 H+ +

N2
+ 16 ATP + 16 H2O = 4 oxidized ferredoxin +
H2
 + 2 NH3 + 16 ADP + 16 phosphate

Synonyms

ARA, dinitrogenase, EC 1.18.2.1, Kp2, Mo nitrogenase, Mo-dependent nitrogenase, Mo-Fe nitrogenase, Mo-nitrogenase, MoFe protein, MoFe-protein, molybdenum nitrogenase, molybdenum-containing nitrogenase, molybdenum-iron protein, molybdenum-nitrogenase, More, N2ase, nif, nif1, nif2, nifD, NifDK, NifH, NifH2, nifHDK, nifK, nifM, nitrogenase Fe protein, nitrogenase Fe-protein, nitrogenase FeVco, nitrogenase iron-protein, nitrogenase MoFe protein, nitrogenase MoFe-protein, nitrogenase molybdenum iron protein, nitrogenase molybdenum-iron protein, V-nitrogenase, vanadium nitrogenase, vanadium-nitrogenase, VnfDGK, VnfH

ECTree

     1 Oxidoreductases
         1.18 Acting on iron-sulfur proteins as donors
             1.18.6 With dinitrogen as acceptor
                1.18.6.1 nitrogenase

Cloned

Cloned on EC 1.18.6.1 - nitrogenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
amino acid sequence comparison with other species
-
expressed in Escherichia coli strain 78-7
-
expression in Escherichia coli
-
expression in Escherichia coli, expression in Klebsiella pneumoniae, Anabaena gene library screening with genes of Klebsiella pneumoniae
-
expression of His-tagged wild-type and V70A mutant MoFe protein in strain DJ1310, expression of the wild-type Fe protein
-
expression of nitrogenase in the absence of NH4+ and at initial O2 concentrations above 5% in the culture atmosphere
-
gene MJ0685, expression of His-tagged NifH2 in Escherichia coli strain BL21 (DE3)
-
genetic analysis
-
overexpression of Azotobacter vinelandii NafY protein in Escherichia coli strain BL21(DE3)
-
phylogenetic analysis, overview
-
recombinant expression of wild-type and mutant gene nifD by gene replacement, Escherichia coli strain HB101(pRL623) and J53 (RP4) are used for the transfer of mobilizable plasmids into Anabaena cells, with a deletion of nifHDK-ORF, by conjugation via triparental mating, subcloning in Escherichia coli strain XL-1 Blue
-
strain YM68A expresses the His-tagged VFe protein, strain YM13A expresses the His-tagged MoFe protein, and strain DJ1143 expresses the His-tagged DELTAnifB MoFe protein
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the Fe subunit of nitrogenase is expressed in Nicotiana tabacum cultivar Petit Havana
-