Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

1.18.6.1: nitrogenase

This is an abbreviated version!
For detailed information about nitrogenase, go to the full flat file.

Word Map on EC 1.18.6.1

Reaction

4 reduced ferredoxin + 8 H+ +

N2
+ 16 ATP + 16 H2O = 4 oxidized ferredoxin +
H2
+ 2 NH3 + 16 ADP + 16 phosphate

Synonyms

ARA, dinitrogenase, EC 1.18.2.1, Kp2, Mo nitrogenase, Mo-dependent nitrogenase, Mo-Fe nitrogenase, Mo-nitrogenase, MoFe protein, MoFe-protein, molybdenum nitrogenase, molybdenum-containing nitrogenase, molybdenum-iron protein, molybdenum-nitrogenase, More, N2ase, nif, nif1, nif2, nifD, NifDK, NifH, NifH2, nifHDK, nifK, nifM, nitrogenase Fe protein, nitrogenase Fe-protein, nitrogenase FeVco, nitrogenase iron-protein, nitrogenase MoFe protein, nitrogenase MoFe-protein, nitrogenase molybdenum iron protein, nitrogenase molybdenum-iron protein, V-nitrogenase, vanadium nitrogenase, vanadium-nitrogenase, VnfDGK, VnfH

ECTree

     1 Oxidoreductases
         1.18 Acting on iron-sulfur proteins as donors
             1.18.6 With dinitrogen as acceptor
                1.18.6.1 nitrogenase

Inhibitors

Inhibitors on EC 1.18.6.1 - nitrogenase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
-
-
1,2-Dihydroxybenzene 3,5-disulfonate
-
-
2,2'-dipyridyl
-
-
2,3-Dimercaptopropanol
-
-
Acetylene
beryllium fluoride
inhibits by trapping of a stable Fe protein-MoFe protein nitrogenase complex
cyanide
glutamine
hydrazine
-
and derivatives
L-asparagine
-
76% inhibition at 10 mM asparagine in the growth medium
L-aspartic acid
-
67% inhibition at 10 mM aspartic acid in the growth medium
L-cysteine
-
51% inhibition at 5 mM cysteine in the growth medium
L-Glutamic acid
-
68% inhibition at 10 mM glutamic acid in the growth medium
L-glutamine
-
60% inhibition at 10 mM glutamine in the growth medium
L-leucine
-
54% inhibition at 5 mM leucine in the growth medium
MgADP
N3-
-
inhibits H2 production competitively and reversibly
NaCl
-
half-maximal inhibition at 100 mM
NAD+-malic enzyme
-
affects nitrogenase activity of Mesorhizobium loti bacteroids in Lotus japonicus nodules, also in Bradyrhizobium japonicum and Sinorhizobium meliloti. nodules, analysis by signature-tagged mutagenesis using transposon insertion malic enzyme mutants, overview
-
NH4Cl
-
nitrogenase activity is repressed by the addition of 0.5 mM NH4Cl
NifI(1) protein
-
NifI(1)/Nifl(2) inhibit activity of purified nitrogenase when present in a 1:1 molar ratio to dinitrogenase, and 2-oxoglutarate is fully relieved this inhibition
-
NifI(2) protein
-
NifI(1)/Nifl(2) inhibit activity of purified nitrogenase when present in a 1:1 molar ratio to dinitrogenase, and 2-oxoglutarate is fully relieved this inhibition
-
NIFI1,2
-
a regulatory protein, inhibits nitrogenase by competing with Fe protein for binding to the MoFe protein, NifI1,2 inhibits ATP- and MoFe protein-dependent oxidation of the Fe protein, and NIFI1,2 binding prevents association of the two nitrogenase components, the inhibition is relieved by 2-oxoglutarate. NIFI1,2 is unable to bind to an AlF4-stabilized Fe protein-MoFe protein complex. Both nifI1 and nifI2 are required for regulation in vivo
-
nitrate
-
involvement of NO production in the inhibition of nitrogenase activity by nitrate , overview. The enzyme of plant symbiont Mesorhizobium loti, strain New Zealand Palmerston 2235, is not inhibited by nitrate
NO3-
-
17% inhibition at 15 mM nitrate in the growth medium
phosphate
-
above 30 mM
SeCN-
-
potent, reversible inhibitor of acetylene reduction
sodium nitroprusside
-
influence of the NO donor sodium nitroprusside on the relative levels of acetylene reduction activity, overview
tetrafluoroaluminate
inhibits by trapping of a stable Fe protein-MoFe protein nitrogenase complex, binds to the Fe protein
tungstate
-
30% inhibition at 0.01 mM, 50% inhibition at 0.1 mM, 95% inhibition at 1 mM, inhibits MoFe protein expression
Urea
-
immediate inhibition, repression of induction
Vanadium
-
contains 3 classes of nitrogenase, the second contains V, the third is encoded by a separate set of genes and is inhibited by V and Mo
VO2+
-
structural basis for VO2+ inhibition of nitrogenase activity, 31P and 23Na interactions with the metal at the nucleotide binding site of the nitrogenase Fe protein identified by ENDOR spectroscopy, vanadyl hyperfine couplings of VO2+-ATP and VO2+-ADP complexes in the presence of the nitrogenase Fe protein, overview
additional information
-