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1,2-Dihydroxybenzene 3,5-disulfonate
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2,3-Dimercaptopropanol
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beryllium fluoride
inhibits by trapping of a stable Fe protein-MoFe protein nitrogenase complex
hydrazine
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and derivatives
L-asparagine
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76% inhibition at 10 mM asparagine in the growth medium
L-aspartic acid
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67% inhibition at 10 mM aspartic acid in the growth medium
L-cysteine
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51% inhibition at 5 mM cysteine in the growth medium
L-Glutamic acid
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68% inhibition at 10 mM glutamic acid in the growth medium
L-glutamine
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60% inhibition at 10 mM glutamine in the growth medium
L-leucine
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54% inhibition at 5 mM leucine in the growth medium
N3-
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inhibits H2 production competitively and reversibly
NaCl
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half-maximal inhibition at 100 mM
NAD+-malic enzyme
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affects nitrogenase activity of Mesorhizobium loti bacteroids in Lotus japonicus nodules, also in Bradyrhizobium japonicum and Sinorhizobium meliloti. nodules, analysis by signature-tagged mutagenesis using transposon insertion malic enzyme mutants, overview
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NH4Cl
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nitrogenase activity is repressed by the addition of 0.5 mM NH4Cl
NifI(1) protein
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NifI(1)/Nifl(2) inhibit activity of purified nitrogenase when present in a 1:1 molar ratio to dinitrogenase, and 2-oxoglutarate is fully relieved this inhibition
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NifI(2) protein
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NifI(1)/Nifl(2) inhibit activity of purified nitrogenase when present in a 1:1 molar ratio to dinitrogenase, and 2-oxoglutarate is fully relieved this inhibition
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NIFI1,2
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a regulatory protein, inhibits nitrogenase by competing with Fe protein for binding to the MoFe protein, NifI1,2 inhibits ATP- and MoFe protein-dependent oxidation of the Fe protein, and NIFI1,2 binding prevents association of the two nitrogenase components, the inhibition is relieved by 2-oxoglutarate. NIFI1,2 is unable to bind to an AlF4-stabilized Fe protein-MoFe protein complex. Both nifI1 and nifI2 are required for regulation in vivo
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nitrate
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involvement of NO production in the inhibition of nitrogenase activity by nitrate , overview. The enzyme of plant symbiont Mesorhizobium loti, strain New Zealand Palmerston 2235, is not inhibited by nitrate
NO3-
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17% inhibition at 15 mM nitrate in the growth medium
SeCN-
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potent, reversible inhibitor of acetylene reduction
sodium nitroprusside
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influence of the NO donor sodium nitroprusside on the relative levels of acetylene reduction activity, overview
tetrafluoroaluminate
inhibits by trapping of a stable Fe protein-MoFe protein nitrogenase complex, binds to the Fe protein
tungstate
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30% inhibition at 0.01 mM, 50% inhibition at 0.1 mM, 95% inhibition at 1 mM, inhibits MoFe protein expression
Urea
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immediate inhibition, repression of induction
Vanadium
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contains 3 classes of nitrogenase, the second contains V, the third is encoded by a separate set of genes and is inhibited by V and Mo
VO2+
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structural basis for VO2+ inhibition of nitrogenase activity, 31P and 23Na interactions with the metal at the nucleotide binding site of the nitrogenase Fe protein identified by ENDOR spectroscopy, vanadyl hyperfine couplings of VO2+-ATP and VO2+-ADP complexes in the presence of the nitrogenase Fe protein, overview
Acetylene
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noncompetitive inhibition of N2 reduction
Acetylene
noncompetitive inhibition of nitrogen reduction, Gly69 is important
ADP
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C2H2
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noncompetitive inhibitor of N2 reduction
C2H2
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noncompetitive inhibitor of N2 reduction
C2H2
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inhibition of H195 mutants
C2H2
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noncompetitive inhibitor of N2 reduction
C2H2
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noncompetitive inhibitor of N2 reduction
C2H2
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noncompetitive inhibitor of N2 reduction
CO
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noncompetitive inhibitor of N2, C2H2 and N3- reduction, no inhibition of H+ reduction
CO
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noncompetitive inhibitor of N2, C2H2 and N3- reduction, no inhibition of H+ reduction
CO
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inhibition of CH4 and NH3 production from CN-
CO
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inhibition of H+ reduction by about 50%
CO
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binds to the active site
CO
strong inhibition, binding site and inhibition mechanism
CO
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noncompetitive inhibitor of N2, C2H2 and N3- reduction, no inhibition of H+ reduction
CO
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noncompetitive inhibitor of N2, C2H2 and N3- reduction, no inhibition of H+ reduction
CO
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noncompetitive inhibitor of N2, C2H2 and N3- reduction, no inhibition of H+ reduction
cyanide
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cyanide
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inhibits electron flow
glutamine
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H2
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H2
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competitive inhibitor of N2, no inhibition of N3-, C2H2, CN- or H+ reduction
H2
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competitive inhibitor of N2, no inhibition of N3-, C2H2, CN- or H+ reduction
H2
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competitive inhibition of N2 binding
H2
H2 competes with N2 binding and inhibits N2 reduction by the FeMo protein, but H2 does not inhibit NO2- reduction for the wild-type or either of the two MoFe protein variants
H2
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competitive inhibitor of N2, no inhibition of N3-, C2H2, CN- or H+ reduction
MgADP
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N2
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inhibits the C2H2 reduction
N2
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inhibits C2H2 reduction of mutant H195Q; maximal inhibition of H2 production at Fe protein to MoFe protein ration 2.5
N2
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inhibits the C2H2 reduction
N2
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competitive inhibition of acetylene reduction
N2
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inhibits hydrazine reduction
NH4+
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immediate inhibition, repression of induction
NH4+
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18% inhibition at 5 mM, 32% at 15 mM ammonium in the growth medium
NH4+
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immediate inhibition, repression of induction
NH4+
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immediate inhibition, repression of induction
O2
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O2
Cyanobacterium sp.
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irreversibly inactivated
O2
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the enzyme is irreversibly inactivated by exposure to even low concentrations of O2
O2
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complete reversible inhibition, reversibility decreases by increasing the time of exposure to O2
SCN-
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above 6 mM occurs substrate inhibition
additional information
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high ionic strength inhibits
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additional information
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e.g. above 50 mM NaCl; high ionic strength inhibits
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additional information
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overview
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additional information
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overview
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additional information
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enzyme activity and bacterial growth is affected by amino acids in the growth medium
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additional information
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overview
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