Crystallization (Comment) | Organism |
---|---|
MoFe protein | Azotobacter vinelandii |
purified enzyme is diluted at room temperature with 3 volumes of Tris-HCl 0.01 M, pH 7.2, immediate crystal formation | Azotobacter vinelandii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | - |
Azotobacter vinelandii | |
1,2-Dihydroxybenzene 3,5-disulfonate | - |
Azotobacter vinelandii | |
2,2'-dipyridyl | - |
Azotobacter vinelandii | |
2,3-Dimercaptopropanol | - |
Azotobacter vinelandii | |
ADP | - |
Azotobacter vinelandii | |
CO | - |
Azotobacter vinelandii | |
Cu2+ | - |
Azotobacter vinelandii | |
H2 | - |
Azotobacter vinelandii | |
additional information | high ionic strength inhibits | Azotobacter vinelandii | |
NO | - |
Azotobacter vinelandii | |
O2 | - |
Azotobacter vinelandii | |
Zn2+ | - |
Azotobacter vinelandii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Azotobacter vinelandii | |
0.1 | - |
N2 | - |
Azotobacter vinelandii | |
0.3 | - |
ATP | - |
Azotobacter vinelandii | |
0.4 | 1.2 | C2H2 | - |
Azotobacter vinelandii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | divalent cation requirement is satisfied by Co2+, is best supported by concentrations of divalent cation one-half the concentration of ATP | Azotobacter vinelandii | |
Fe2+ | divalent cation requirement is satisfied by Fe2+, is best supported by concentrations of divalent cation one-half the concentration of ATP | Azotobacter vinelandii | |
Mg2+ | divalent metal requirement is satisfied by Mg2+, reaction is best supported by concentration of divalent cation one-half the concentration of ATP | Azotobacter vinelandii | |
Mn2+ | divalent cation requirement is satisfied by Mn2+, is best supported by concentrations of divalent cation one-half the concentration of ATP | Azotobacter vinelandii | |
Ni2+ | divalent cation requirement is satisfied by Ni2+, is best supported by concentrations of divalent cation one-half the concentration of ATP | Azotobacter vinelandii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O | Azotobacter vinelandii | - |
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Azotobacter vinelandii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Azotobacter vinelandii |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate | enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein | Azotobacter vinelandii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Azotobacter vinelandii |
Storage Stability | Organism |
---|---|
-15°C, anaerobic storage, overnight, complete loss of activity | Azotobacter vinelandii |
22°C, O2-free atmosphere, pH 7-8, stable | Azotobacter vinelandii |
5°C, O2-free atmosphere, overnight, 80% loss of activity | Azotobacter vinelandii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 reduced ferredoxin + 2 H+ + acetylene + 2 ATP + 2 H2O | - |
Azotobacter vinelandii | 2 oxidized ferredoxin + ethylene + 2 ADP + 2 phosphate | - |
? | |
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O | - |
Azotobacter vinelandii | 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate | - |
? | |
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O | 1-propyne, 1-butyne and allene are reduced to the corresponding alkenes | Azotobacter vinelandii | 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate | - |
? | |
reduced ferredoxin + H+ + CH3NC + ATP | - |
Azotobacter vinelandii | oxidized ferredoxin + CH4 + C2H4 + C3H6 + C3H8 + CH3NH2 + ADP + phosphate | - |
? | |
reduced ferredoxin + H+ + N3- + ATP | - |
Azotobacter vinelandii | oxidized ferredoxin + NH3 + N2 + ADP + phosphate | - |
? |