1.18.6.1: nitrogenase

This is an abbreviated version!
For detailed information about nitrogenase, go to the full flat file.

Reaction

4 reduced ferredoxin + 8 H+ +

Synonyms

ARA, dinitrogenase, EC 1.18.2.1, Kp2, Mo nitrogenase, Mo-dependent nitrogenase, Mo-Fe nitrogenase, Mo-nitrogenase, MoFe protein, MoFe-protein, molybdenum nitrogenase, molybdenum-containing nitrogenase, molybdenum-iron protein, molybdenum-nitrogenase, More, N2ase, nif, nif1, nif2, nifD, NifDK, NifH, NifH2, nifHDK, nifK, nifM, nitrogenase Fe protein, nitrogenase Fe-protein, nitrogenase FeVco, nitrogenase iron-protein, nitrogenase MoFe protein, nitrogenase MoFe-protein, nitrogenase molybdenum iron protein, nitrogenase molybdenum-iron protein, V-nitrogenase, vanadium nitrogenase, vanadium-nitrogenase, VnfDGK, VnfH

ECTree

     1 Oxidoreductases

         1.18 Acting on iron-sulfur proteins as donors

             1.18.6 With dinitrogen as acceptor

                1.18.6.1 nitrogenase

Advanced search results

Do not include text mining results

Include results (more...)

Include results (more...)

Results	in table
12	Activating Compound
18192	AA Sequence
4	Application
1	CAS Registry Number
16	Cloned(Commentary)
70	Cofactor
16	Crystallization (Commentary)
24	General Information
2	General Stability
125	Inhibitors
5	Ki Value [mM]
33	KM Value [mM]
13	Localization
445	Metals/Ions
138	Molecular Weight [Da]
204	Natural Substrates/ Products (Substrates)
311	Organism
54	Oxidation Stability
7	Pathway
412	PDB ID
14	pH Optimum
3	pH Range
3	pH Stability
2	Posttranslational Modification
112	Protein Variants
40	Purification (Commentary)
214	Reaction
3	Reaction Type
246	Reference
2	Renatured (Commentary)
17	Source Tissue
86	Specific Activity [micromol/min/mg]
4	Storage Stability
591	Substrates and Products (Substrate)
148	Subunits
88	Synonyms
1	Systematic Name
16	Temperature Optimum [°C]
3	Temperature Range [°C]
2	Temperature Stability [°C]
3	Turnover Number [1/s]

Organism

print visible entries

print all entries

Go back to the abbreviated version
of the Enzyme Summary Page.

Organism on EC 1.18.6.1 - nitrogenase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.

top print hide show all columns Go to Organism Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

ORGANISM

COMMENTARY

LITERATURE

UNIPROT

SEQUENCE DB

SOURCE

Allochromatium vinosum

-

440142, 440143, 440144, 440145

-

-

brenda

Alnus maritima

enzyme activity increases with increasing concentration of O2 in the root zone. Photosynthetic rate, plant dry mass, leaf N content, and nodule fresh mass are maximal in plants maintained with 15-25% O2 in the root zone

676442

-

-

brenda

Anabaena cylindrica

-

440134, 440144, 440145, 440170, 744073

-

-

brenda

Anabaena cylindrica B1611

-

744073

-

-

brenda

Anabaena cylindrica F243

-

744073

-

-

brenda

Anabaena sp.

-

440134, 440166, 713837

-

-

brenda

Anabaena sp. PCC 7120

-

713837

-

-

brenda

Azoarcus sp.

-

698619

-

-

brenda

Azoarcus sp. BH72

-

698619

-

-

brenda

Azospirillum brasilense

-

440166, 673723

-

-

brenda

Azospirillum sp.

-

440134

-

-

brenda

Azotobacter chroococcum

3 entries

Azotobacter chroococcum YM68A

contains Mo- and V-nitrogenases

715261

-

-

brenda

Azotobacter sp.

-

440134, 440166

-

-

brenda

Azotobacter vinelandii

16 entries

Azotobacter vinelandii DJ

-

659338

-

-

brenda

Azotobacter vinelandii DJ1143

-

728685

-

-

brenda

Azotobacter vinelandii DJ1260

-

727875, 728683, 728690

-

-

brenda

Azotobacter vinelandii DJ1264

alpha and beta chains

745506

P07328; P07329

UniProt

brenda

Azotobacter vinelandii DJ1310

-

658010

-

-

brenda

Azotobacter vinelandii DJ995

-

688191, 726970, 727005, 745163

-

-

brenda

Azotobacter vinelandii OP

-

440180, 727683

-

-

brenda

Beggiatoa alba

-

440160

-

-

brenda

Beggiatoa alba B18LD

-

440160

-

-

brenda

Bradyrhizobium japonicum

4 entries

Bradyrhizobium lupini

2 entries

Cereibacter sphaeroides

-

440134

-

-

brenda

Chlorobium sp.

-

440134

-

-

brenda

Chromatium sp.

-

440134, 440174

-

-

brenda

Clostridium pasteurianum

3 entries

Corynebacterium flavescens

2 entries

Corynebacterium flavescens 301

301

440145

-

-

brenda

Crocosphaera watsonii

a marine unicellular diazotrophic cyanobacterium

714845

-

-

brenda

Crocosphaera watsonii WH8501

a marine unicellular diazotrophic cyanobacterium

714845

-

-

brenda

Cyanobacterium sp.

-

440148

-

-

brenda

Desulfovibrio desulfuricans

low activity

440145

-

-

brenda

Desulfovibrio sp.

-

440134

-

-

brenda

Dysgonomonas capnocytophagoides

-

745127

-

-

brenda

Escherichia coli

C-M 74

440145

-

-

brenda

Escherichia coli C-M 74

C-M 74

440145

-

-

brenda

Fischerella sp.

-

746480

-

-

brenda

Frankia sp.

-

440134, 440166

-

-

brenda

Gloeocapsa sp.

-

440145

-

-

brenda

Gloeothece sp.

2 entries

Gloeothece sp. PCC6909

a terrestrial unicellular diazotrophic cyanobacterium

714845

-

-

brenda

Gluconacetobacter diazotrophicus

2 entries

Gluconacetobacter diazotrophicus PAL 5

in endosymbiosis with sugarcane, Saccharum officinarum

658372

-

-

brenda

Herbaspirillum seropedicae

-

658769

-

-

brenda

Klebsiella oxytoca

-

745127

-

-

brenda

Klebsiella pneumoniae

3 entries

Klebsiella pneumoniae M5a1

-

658901

-

-

brenda

Klebsiella pneumoniae UN1217

-

715017

-

-

brenda

Lacrimispora aerotolerans

-

745127

-

-

brenda

Leptolyngbya boryana

-

440134, 440145

-

-

brenda

Lotus japonicus

inoculated with symbiont Mesorhizobium loti strain New Zealand Palmerston 2235

699812

-

-

brenda

Mesorhizobium loti

-

716483

-

-

brenda

Methanocaldococcus jannaschii

NifH2 is one of two homologues of NifH in Methanocaldococcus jannaschii

713656

-

-

brenda

Methanococcus maripaludis

-

676049, 684881, 728654

-

-

brenda

Methanosarcina barkeri

-

727719

-

-

brenda

Methanosarcina barkeri 227

-

727719

-

-

brenda

Nitrospirillum amazonense

-

440161

-

-

brenda

Nitrospirillum amazonense Y1

-

440161

-

-

brenda

Nostoc sp.

-

744073

-

-

brenda

Nostoc sp. F280

-

744073

-

-

brenda

Ornithopus sativus

-

440145

-

-

brenda

Oscillatoria sp.

-

440166

-

-

brenda

Paenibacillus polymyxa

-

440134, 440143, 440144, 440145, 440146, 440147, 440169, 745705

-

-

brenda

Rhizobium leguminosarum

-

440134

-

-

brenda

Rhizobium sp.

4 entries

Rhizobium sp. ORS 571

ORS571

440134

-

-

brenda

Rhodobacter capsulatus

4 entries

Rhodobacter capsulatus B10S

-

440176, 440177

-

-

brenda

Rhodopseudomonas palustris

-

721351

-

-

brenda

Rhodopseudomonas sp.

-

440134

-

-

brenda

Rhodospirillum rubrum

-

440134, 440142, 440143, 440144, 440145, 440166, 662028

-

-

brenda

Stenotrophomonas sp. gx-44

-

745127

-

-

brenda

Trichormus variabilis

2 entries

Xanthobacter autotrophicus

-

440168

-

-

brenda

Azotobacter chroococcum

-

440143, 440144, 440145, 440146, 440150, 440166, 440167

-

-

brenda

Azotobacter chroococcum

contains Mo- and V-nitrogenases

715261

-

-

brenda

Azotobacter chroococcum

nitrogen fixation complex is encoded on nif gene cluster

440186

-

-

brenda

Azotobacter vinelandii

-

440134, 440138, 440142, 440143, 440144, 440145, 440146, 440147, 440153, 440154, 440156, 440158, 440163, 440166, 440174, 440175, 440178, 440180, 440181, 440183, 440184, 440187, 440189, 658010

-

-

brenda

Azotobacter vinelandii

-

658483

P00459

Uniprot

brenda

Azotobacter vinelandii

-

659338, 659426, 672039, 673039, 674147, 674154, 676778, 688191, 689764, 714796, 715263

-

-

brenda

Azotobacter vinelandii

-

715813

P07328

UniProt

brenda

Azotobacter vinelandii

-

716924, 726970, 727005, 727683, 727875, 728672, 728683, 728685, 728690

-

-

brenda

Azotobacter vinelandii

-

744320

P07328

UniProt

brenda

Azotobacter vinelandii

-

744667, 744780, 744940, 745163, 745173, 745384, 745841, 746211, 746301, 746495

-

-

brenda

Azotobacter vinelandii

2 forms of VFe protein

440186, 440188

-

-

brenda

Azotobacter vinelandii

alpha and beta chains

744333, 745155, 745505, 745506

P07328; P07329

UniProt

brenda

Azotobacter vinelandii

contains 3 classes of nitrogenase, the second contains V, the third is encoded by a separate set of genes and is lacking V and Mo and is inhibited by V and Mo

440150, 440155

-

-

brenda

Azotobacter vinelandii

contains nif-encoded molybdenum nitrogenase and vnf-encoded V nitrogenase

716922

-

-

brenda

Azotobacter vinelandii

molybdenum-dependent enzyme variant

658040

-

-

brenda

Azotobacter vinelandii

nitrogen fixation complex is encoded on nif gene cluster

440179, 440182, 440186

-

-

brenda

Azotobacter vinelandii

recombinant strain DJ1373 expressing mutant V70I

689764

-

-

brenda

Azotobacter vinelandii

several strains

742740

P00459

Uniprot

brenda

Azotobacter vinelandii

theoretical investigation of the binding of N2 to the Fe7MoS9N(homocitrate)(cysteine)(histidine) active site, calculation of reaction profiles and activation energies for the association and dissociation of N2

674202

-

-

brenda

Bradyrhizobium japonicum

-

440143, 440144

-

-

brenda

Bradyrhizobium japonicum

associated with Glycine max

440145

-

-

brenda

Bradyrhizobium japonicum

nitrogen fixation complex is encoded on nif gene cluster

440151

-

-

brenda

Bradyrhizobium japonicum

strains 110 or 2134, anaerobically and aerobically isolated bacteroid from nodules of soybean

660287

-

-

brenda

Bradyrhizobium lupini

-

440143, 440144, 440165

-

-

brenda

Bradyrhizobium lupini

associated with Lupinus luteus

440145

-

-

brenda

Clostridium pasteurianum

-

440134, 440142, 440143, 440144, 440145, 440146, 440147, 440153, 440159, 440164, 440174, 440180, 745173, 745384

-

-

brenda

Clostridium pasteurianum

alpha and beta chains

744333

P00467; P11347

UniProt

brenda

Clostridium pasteurianum

nitrogen fixation complex is encoded on nif gene cluster

440186

-

-

brenda

Corynebacterium flavescens

-

440144

-

-

brenda

Corynebacterium flavescens

301

440145

-

-

brenda

Gloeothece sp.

-

440134, 440166

-

-

brenda

Gloeothece sp.

a terrestrial unicellular diazotrophic cyanobacterium

714845

-

-

brenda

Gluconacetobacter diazotrophicus

expression of nitrogenase in the absence of NH4+ and at initial O2 concentrations above 5% in the culture atmosphere

672344

-

-

brenda

Gluconacetobacter diazotrophicus

in endosymbiosis with sugarcane, Saccharum officinarum

658372

-

-

brenda

Klebsiella pneumoniae

-

440134, 440140, 440142, 440143, 440144, 440145, 440146, 440149, 440157, 440166, 440174, 658901, 687822, 687823, 715017

-

-

brenda

Klebsiella pneumoniae

alpha and beta chains

744333

P00466; P09772

UniProt

brenda

Klebsiella pneumoniae

nitrogen fixation complex is encoded on nif gene cluster

440147, 440152

-

-

brenda

Rhizobium sp.

-

440166, 440174

-

-

brenda

Rhizobium sp.

associated with Phaseolus aureus or Vigna sinensis

440145

-

-

brenda

Rhizobium sp.

ORS571

440134

-

-

brenda

Rhizobium sp.

ORS571, associated with Sesbania rostrata

440162

-

-

brenda

Rhodobacter capsulatus

-

440142, 440171, 440176, 440177

-

-

brenda

Rhodobacter capsulatus

iron-only nitrogenase and molybdenum nitrogenase

440176, 440177

-

-

brenda

Rhodobacter capsulatus

nitrogen fixation complex is encoded on nif gene cluster

440176, 440177

-

-

brenda

Rhodobacter capsulatus

wild-type strain B10S, molybdenum-containing, nif-encoded enzyme variant

657985

-

-

brenda

Trichormus variabilis

-

440148, 440166, 745223

-

-

brenda

Trichormus variabilis

nitrogen fixation complex is encoded on nif gene cluster

440152

-

-

brenda