Protein Variants | Comment | Organism |
---|---|---|
H195G | alpha-His of MoFe protein, site directed mutagenesis, reduced MoFe protein activity, slightly decreased Fe protein activity, altered phenotype | Azotobacter vinelandii |
H195L | alpha-His of MoFe protein, site directed mutagenesis, reduced MoFe protein activity, increased Fe protein activity, altered phenotype | Azotobacter vinelandii |
H195N | alpha-His of MoFe protein, site directed mutagenesis, reduced MoFe protein activity, altered phenotype | Azotobacter vinelandii |
H195Q | alpha-His of MoFe protein, site directed mutagenesis, decreased MoFe protein activity, altered phenotype | Azotobacter vinelandii |
H195T | alpha-His of MoFe protein, site directed mutagenesis, reduced MoFe protein and Fe protein activity, altered phenotype | Azotobacter vinelandii |
H195Y | alpha-His of MoFe protein, site directed mutagenesis, reduced MoFe protein and Fe protein activity, altered phenotype | Azotobacter vinelandii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CO | inhibition of H+ reduction by about 50% | Azotobacter vinelandii | |
N2 | inhibits the C2H2 reduction | Azotobacter vinelandii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Azotobacter vinelandii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | structure and organization of metal clusters | Azotobacter vinelandii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Azotobacter vinelandii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
wild-type and mutant H195Q | Azotobacter vinelandii |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate | enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein | Azotobacter vinelandii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
wild-type and diverse alpha-His195 MoFe protein mutants | Azotobacter vinelandii |
additional information | - |
assay in anaerobic atmosphere required | Azotobacter vinelandii |
0.066 | - |
crude extract, 100% Ar atmosphere, H2 production | Azotobacter vinelandii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 reduced ferredoxin + 2 H+ + acetylene + 2 ATP + 2 H2O | anaerobic atmosphere | Azotobacter vinelandii | 2 oxidized ferredoxin + ethylene + 2 ADP + 2 phosphate | reduction cycle continues until complete reduction of the substrate to ethane | ? | |
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O | - |
Azotobacter vinelandii | 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate | - |
? | |
reduced ferredoxin + H+ + ATP | - |
Azotobacter vinelandii | oxidized ferredoxin + H2 + ADP + phosphate | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | structure of MoFe-cofactor | Azotobacter vinelandii |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Azotobacter vinelandii |