1.1.1.219: dihydroflavonol 4-reductase

This is an abbreviated version, for detailed information about dihydroflavonol 4-reductase, go to the full flat file.

Reaction

a (2R,3S,4S)-leucoanthocyanidin
+
NADP+
=
a (2R,3R)-dihydroflavonol
+
NADPH
+
H+

Synonyms

DFR, DFR1, DFR2, DFR3, DFR4a, DFR4b, DFR5, DHM reductase, DHQ reductase, dihydroflavanol 4-reductase, dihydroflavonol 4-reductase, dihydroflavonol reductase, dihydroflavonol-4-reductase, dihydrokaempferol 4-reductase, dihydromyricetin reductase, dihydroquercetin reductase, NADPH-dihydromyricetin reductase, PAS_chr3_0449, reductase, dihydromyricetin, reductase, dihydroquercetin, TRANSPARENT TESTA 3 protein

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.219 dihydroflavonol 4-reductase

Specific Activity

Specific Activity on EC 1.1.1.219 - dihydroflavonol 4-reductase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.000012
-
pigmented cultivar Tarocco in 0.05 M Tris-HCl pH 7.5, l M dihydroquercetin, 2 mM NADPH, 1 U glucose-6-phosphate dehydrogenase, 6 mM glucose-6-phosphate and enzyme extract at 25C
0.00002
with dihydrokaempferol as substrate
0.0003
with dihydrokaempferol as substrate; with naringenin as substrate
0.0004
with dihydrokaempferol as substrate; with naringenin as substrate
0.0005
with naringenin as substrate
0.0006
0.0007
0.0008
-
with eriodictyol as substrate
0.0012
0.0013
0.0014
0.0015
with dihydromyricetin as substrate
0.014
with dihydrokaempferol as substrate
0.021
-
with dihydrokaempferol as substrate
0.024
-
-
0.042
-
sample callus control in 25 mM Tris-HCl (pH 7.0), 4 mM NADPH and 0.1 mM dihydroquercetin at 25C
0.103
-
sample callus L-1 in 25 mM Tris-HCl (pH 7.0), 4 mM NADPH and 0.1 mM dihydroquercetin at 25C
0.155
-
sample callus L-2 in 25 mM Tris-HCl (pH 7.0), 4 mM NADPH and 0.1 mM dihydroquercetin at 25C
0.202
-
sample plant control in 25 mM Tris-HCl (pH 7.0), 4 mM NADPH and 0.1 mM dihydroquercetin at 25C
0.268
-
sample plant L-2 in 25 mM Tris-HCl (pH 7.0), 4 mM NADPH and 0.1 mM dihydroquercetin at 25C
0.349
-
sample plant L-1 in 25 mM Tris-HCl (pH 7.0), 4 mM NADPH and 0.1 mM dihydroquercetin at 25C
156.4
-
without any fruit treatment, day 4 of storage at 1C
42850
recombinant protein
additional information