BRENDA - Enzyme Database show
show all sequences of 1.1.1.219

Biosynthesis of flavan-3-ols by leaf extracts of Onobrychis viciifolia

Singh, S.; McCallum, J.; Gruber, M.Y.; Towers, G.H.N.; Muir, A.D.; Bohm, B.A.; Koupai-Abyazani, M.R.; Glass, A.D.M.; Phytochemistry 44, 425-432 (1997)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
diethyldithiocarbamate
50% inhibition at 2 mM
Onobrychis viciifolia
dithiothreitol
50% inhibition at 5 mM
Onobrychis viciifolia
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Onobrychis viciifolia
biosynthesis of proanthocyanidin polymers (condensed tannins)
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Onobrychis viciifolia
-
sainfoin, cv. Melrose
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
-
Onobrychis viciifolia
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
measurement of reductase activity during leaf development
Onobrychis viciifolia
Storage Stability
Storage Stability
Organism
5°C, stable for 36 h
Onobrychis viciifolia
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(+)-dihydromyricetin + NADPH
i.e. 5'-hydroxy-dihydroquercetin
348000
Onobrychis viciifolia
cis-3,4-leucodelphinidin + NADP+
i.e. 5,7,4'-trihydroxyflavan-3,4-cis-diol
348000
Onobrychis viciifolia
-
(+)-dihydromyricetin + NADPH
i.e. 5'-hydroxy-dihydroquercetin
348000
Onobrychis viciifolia
cis-3,4-leucodelphinidin + NADP+
configuration 2R,3S-trans-3S,4S-cis-leucodelphinidin
348000
Onobrychis viciifolia
-
(+)-dihydroquercetin + NADPH
-
348000
Onobrychis viciifolia
cis-3,4-leucocyanidin + NADP+
i.e. 5,7,3',4'-tetrahydroxyflavan-3,4-cis-diol, 2,3-trans-configuration retained
348000
Onobrychis viciifolia
-
additional information
biosynthesis of proanthocyanidin polymers (condensed tannins)
348000
Onobrychis viciifolia
?
-
-
-
-
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
-
Onobrychis viciifolia
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6.4
7.4
-
Onobrychis viciifolia
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
reduced activity compared to NADPH
Onobrychis viciifolia
NADPH
-
Onobrychis viciifolia
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
reduced activity compared to NADPH
Onobrychis viciifolia
NADPH
-
Onobrychis viciifolia
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
diethyldithiocarbamate
50% inhibition at 2 mM
Onobrychis viciifolia
dithiothreitol
50% inhibition at 5 mM
Onobrychis viciifolia
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Onobrychis viciifolia
biosynthesis of proanthocyanidin polymers (condensed tannins)
?
-
-
-
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
-
Onobrychis viciifolia
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
measurement of reductase activity during leaf development
Onobrychis viciifolia
Storage Stability (protein specific)
Storage Stability
Organism
5°C, stable for 36 h
Onobrychis viciifolia
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(+)-dihydromyricetin + NADPH
i.e. 5'-hydroxy-dihydroquercetin
348000
Onobrychis viciifolia
cis-3,4-leucodelphinidin + NADP+
i.e. 5,7,4'-trihydroxyflavan-3,4-cis-diol
348000
Onobrychis viciifolia
-
(+)-dihydromyricetin + NADPH
i.e. 5'-hydroxy-dihydroquercetin
348000
Onobrychis viciifolia
cis-3,4-leucodelphinidin + NADP+
configuration 2R,3S-trans-3S,4S-cis-leucodelphinidin
348000
Onobrychis viciifolia
-
(+)-dihydroquercetin + NADPH
-
348000
Onobrychis viciifolia
cis-3,4-leucocyanidin + NADP+
i.e. 5,7,3',4'-tetrahydroxyflavan-3,4-cis-diol, 2,3-trans-configuration retained
348000
Onobrychis viciifolia
-
additional information
biosynthesis of proanthocyanidin polymers (condensed tannins)
348000
Onobrychis viciifolia
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
-
Onobrychis viciifolia
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6.4
7.4
-
Onobrychis viciifolia
Other publictions for EC 1.1.1.219
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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3
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1
15
1
1
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3
1
1
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725929
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Mol. Biol. Rep.
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2991-2999
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1
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6
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5
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6
1
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6
1
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3
3
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726292
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Populus trichocarpa
PLoS ONE
7
e30364
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1
2
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704148
Cao
Effect of BTH on anthocyanin c ...
Fragaria x ananassa
J. Agric. Food Chem.
58
5801-5805
2010
1
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2
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1
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1
1
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-
700794
Eungwanichayapant
Accumulation of catechins in t ...
Camellia sinensis var. sinensis
Plant Physiol. Biochem.
47
94-97
2009
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2
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2
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1
1
-
-
-
702936
Akhov
-
Proanthocyanidin biosynthesis ...
Brassica napus
Botany
87
616-625
2009
-
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-
-
-
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-
-
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1
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1
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1
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1
1
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705520
Chemler
Improving NADPH availability f ...
Anthurium andraeanum
Metab. Eng.
12
96-104
2009
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1
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2
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1
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1
1
-
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-
706155
Singh
-
Characterization of dihydrofla ...
Camellia sinensis, Camellia sinensis (L.) O. Kuntze
Plant Biotechnol. Rep.
3
95-101
2009
-
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1
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2
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1
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2
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3
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1
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1
2
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3
1
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1
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2
1
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2
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2
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4
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1
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2
2
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3
2
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2
2
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-
4
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706810
Zhang
The potato R locus codes for d ...
Solanum tuberosum
Theor. Appl. Genet.
119
931-937
2009
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1
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4
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1
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1
1
-
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713265
Yong
-
Agrobacterium-mediated transfo ...
Torenia fournieri
Plant Cell Tissue Organ Cult.
96
59-67
2009
-
1
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1
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713530
Jung
The potato developer (D) locus ...
Solanum tuberosum
Theor. Appl. Genet.
120
45-57
2009
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1
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4
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1
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1
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1
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1
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701213
Nakatsuka
-
Isolation and expression analy ...
Rhododendron x pulchrum
Sci. Hortic.
118
314-320
2008
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1
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1
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4
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4
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1
1
1
1
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701463
Trabelsi
Structural evidence for the in ...
Vitis vinifera
Acta Crystallogr. Sect. D
64
883-891
2008
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1
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2
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4
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1
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702000
Leonard
-
Characterization of dihydrofla ...
Anthurium andraeanum, Arabidopsis thaliana, Fragaria x ananassa, Ipomoea nil, Lilium sp., Rosa hybrid cultivar
Biocatal. Biotransform.
26
243-251
2008
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6
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6
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6
24
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30
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1
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1
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6
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6
6
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6
24
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30
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1
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1
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6
6
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705358
Lee
-
Molecular cloning and biochemi ...
Brassica rapa subsp. pekinensis
J. Plant Biol.
51
42-47
2008
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1
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1
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1
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688073
Zhou
Ultraviolet A-specific inducti ...
Brassica rapa
J. Exp. Bot.
58
1771-1781
2007
1
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1
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688348
Petit
Crystal structure of grape dih ...
Vitis vinifera
J. Mol. Biol.
368
1345-1357
2007
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1
1
1
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1
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1
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1
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1
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5
1
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667201
Takahashi
Evaluation of metabolic altera ...
Oryza sativa
Ann. Bot.
98
819-825
2006
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2
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1
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1
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670520
Lo Piero
Gene characterization, analysi ...
Citrus sinensis
Phytochemistry
67
684-695
2006
-
-
-
-
-
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1
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2
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1
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1
2
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1
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2
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1
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1
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669664
Shimada
A comprehensive analysis of si ...
Lotus japonicus
J. Exp. Bot.
56
2573-2585
2005
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1
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5
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4
-
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1
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5
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1
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1
1
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5
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1
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5
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