1.1.1.219: dihydroflavonol 4-reductase

This is an abbreviated version, for detailed information about dihydroflavonol 4-reductase, go to the full flat file.

Reaction

a (2R,3S,4S)-leucoanthocyanidin
+
NADP+
=
a (2R,3R)-dihydroflavonol
+
NADPH
+
H+

Synonyms

BrDFR1, BrDFR10, BrDFR11, BrDFR12, BrDFR2, BrDFR3, BrDFR4, BrDFR5, BrDFR6, BrDFR7, BrDFR8, BrDFR9, CsDFR, DcDFR, DFR, DFR1, DFR2, DFR3, DFR4a, DFR4b, DFR5, DgDFR, DHM reductase, DHQ reductase, dihydroflavanol 4-reductase, dihydroflavonol 4-reductase, dihydroflavonol reductase, dihydroflavonol-4-reductase, dihydrokaempferol 4-reductase, dihydromyricetin reductase, dihydroquercetin reductase, DnDFR, flavan-3-ol NADPH-dihydromycertin reductase, GbDFR1, GbDFR2, GbDFR3, IbDFR, McDFR, NADPH-dihydromyricetin reductase, PAS_chr3_0449, reductase, dihydromyricetin, reductase, dihydroquercetin, TaDFR, TaDFR-A, TaDFR-I, TRANSPARENT TESTA 3 protein

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.219 dihydroflavonol 4-reductase

Cloned

Cloned on EC 1.1.1.219 - dihydroflavonol 4-reductase

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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
allele of DFR associated with red color, under the control of a doubled CaMV 35S promoter and tobacco etch virus translational enhancer introduced into the potato cultivar Prince Hairy (genotype dddd rrrr P-), which has white tubers and pale blue flowers
DFR2, DFR3 and DFR5 were expressed in Escherichia coli
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enzyme expression analysis under UV-light irradiation, overview
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Escherichia coli strains transformed with plasmid pTrcHis2-DFR to only express DFR. Escherichia coli strains harboring plasmid pET-DFR-LAR expressing DFR and leucoanthocyanidin reductase
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expression in Escherichia coli
expression in Escherichia coli JM109
expression in Escherichia coli; into expression vector pQE-30 UA, and transformed into Escherichia coli M15 pREP-4 competent cells
expression in Nicotiana tabacum; expression in Nicotiana tabacum
expression in Saccharomyces cerevisiae; gene SmDFR, DNA and amino acid sequence determination and analysis, phylogenetic analysis, quantitative real-time PCR expression analysis, functional expression in Saccharomyces cerevisiae strain INV Sc1
functional expression in tobacco protoplasts via electroporation, subcloning and overexpression in Escherichia coli strain GI724 and in Saccharomyces cerevisiae strain INV Sc1, the recombinant Escherichia coli strain shows no enzyme activity
gene BrDFR10, on chromosome A06, genotyping, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, RT-PCR expression profiling of genotypes, and real-time quantitative PCR analysis; gene BrDFR11, on chromosome A03, genotyping, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, RT-PCR expression profiling of genotypes, and real-time quantitative PCR analysis; gene BrDFR12, on chromosome A08, genotyping, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, RT-PCR expression profiling of genotypes, and real-time quantitative PCR analysis; gene BrDFR1, on chromosome A02, genotyping, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, RT-PCR expression profiling of genotypes, and real-time quantitative PCR analysis; gene BrDFR2, on chromosome A09, genotyping, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, RT-PCR expression profiling of genotypes, and real-time quantitative PCR analysis; gene BrDFR3, genotyping, DNA and amino acid sequence determination and analysis, sequence comparisons, RT-PCR expression profiling of genotypes, and real-time quantitative PCR analysis; gene BrDFR3, on chromosome A02, genotyping, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, RT-PCR expression profiling of genotypes, and real-time quantitative PCR analysis; gene BrDFR4, on chromosome A09, genotyping, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, RT-PCR expression profiling of genotypes, and real-time quantitative PCR analysis; gene BrDFR5, on chromosome A09, genotyping, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, RT-PCR expression profiling of genotypes, and real-time quantitative PCR analysis; gene BrDFR6, on chromosome A09, genotyping, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, RT-PCR expression profiling of genotypes, and real-time quantitative PCR analysis; gene BrDFR7, on chromosome A09, genotyping, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, RT-PCR expression profiling of genotypes, and real-time quantitative PCR analysis; gene BrDFR8, on chromosome A06, genotyping, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, RT-PCR expression profiling of genotypes, and real-time quantitative PCR analysis; gene BrDFR9, on chromosome A06, genotyping, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, RT-PCR expression profiling of genotypes, and real-time quantitative PCR analysis
gene CsDFR, recombinant overexpression in Nicotiana tabacum cv. Xanthi leaves
gene DcDFR, genotyping, quantitative enzyme expression analysis
gene DFR, cloned from the capitulum, DNA and amino acid sequence determination and analysis, quantitative RT-PCR expression analysis
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gene DFR, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, functional recombinant expression in Saccharomyces cerevisiae; gene DFR, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, functional recombinant expression in Saccharomyces cerevisiae; gene DFR, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, functional recombinant expression in Saccharomyces cerevisiae; gene DFR, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, functional recombinant expression in Saccharomyces cerevisiae
gene DFR, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, functional recombinant expression in Saccharomyces cerevisiae; gene DFR, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, functional recombinant expression in Saccharomyces cerevisiae; gene DFR, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, functional recombinant expression in Saccharomyces cerevisiae; gene DFR, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, functional recombinant expression in Saccharomyces cerevisiae; gene DFR, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, functional recombinant expression in Saccharomyces cerevisiae; gene DFR, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, functional recombinant expression in Saccharomyces cerevisiae
gene DgDFR, genotyping, quantitative enzyme expression analysis
gene DnDFR, genotyping, quantitative enzyme expression analysis
gene GbDFR1, DNA and amino acid sequence determination and analysis, sequence comparison, phylogenetic analysis, quantitative isozyme expression analysis; gene GbDFR2, DNA and amino acid sequence determination and analysis, sequence comparison, phylogenetic analysis, quantitative isozyme expression analysis; gene GbDFR3, DNA and amino acid sequence determination and analysis, sequence comparison, phylogenetic analysis, quantitative isozyme expression analysis
gene IbDFR, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR enzyme expression analysis
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gene McDFR, relative enzyme expression analysis and quantitative RT-PCR enzyme expression analysis, transient overexpression of McDFR in leaves of cultivars Royalty and Flame. McDFR is overexpressed in fruits using the vector pBI121-McDFR
gene TaDFR-A, from cv. Iksan370, DNA and amino acid sequencdetermination and analysis, quantitative PCR enzyme expression analysis, overexpressing the Triticum aestivum dihydroflavonol 4-reductase gene TaDFR under the control of the CaMV35S promoter via Agrobacterium tumefaciens strain GV3101 (C58) transfection in a Arabidopsis thaliana dfr mutant increases anthocyanin accumulation in the mutant
genetic mapping and genotyping, the gene encoding dihydroflavonol 4-reductase is a candidate for the anthocyaninless locus of rapid cycling Brassica rapa (fast plants type)
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into pTrcHis2-TOPO and heterologously expressed in Escherichia coli TOP10F' strain. DFR cDNA cloned into pRSF-FHT and inserted into Escherichia coli BL21Star to create E-color strain
isozyme MtDFR1, from a young seed cDNA library, functional expression in Escherichia coli; isozyme MtDFR2, from a young seed cDNA library, functional expression in Escherichia coli
overexpression in Escherichia coli strain GI724 and in Saccharomyces cerevisiae strain INV Sc1
quantitative real-time PCR enzyme expression analysis
vector pC1-DFR overexpressed in transgenic tobacco plants, having distinctive petal colors, showing some variation in their intensity