1.1.1.219: dihydroflavonol 4-reductase

This is an abbreviated version, for detailed information about dihydroflavonol 4-reductase, go to the full flat file.

Reaction

a (2R,3S,4S)-leucoanthocyanidin
+
NADP+
=
a (2R,3R)-dihydroflavonol
+
NADPH
+
H+

Synonyms

DFR, DFR1, DFR2, DFR3, DFR4a, DFR4b, DFR5, DHM reductase, DHQ reductase, dihydroflavanol 4-reductase, dihydroflavonol 4-reductase, dihydroflavonol reductase, dihydroflavonol-4-reductase, dihydrokaempferol 4-reductase, dihydromyricetin reductase, dihydroquercetin reductase, NADPH-dihydromyricetin reductase, PAS_chr3_0449, reductase, dihydromyricetin, reductase, dihydroquercetin, TRANSPARENT TESTA 3 protein

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.219 dihydroflavonol 4-reductase

Cloned

Cloned on EC 1.1.1.219 - dihydroflavonol 4-reductase

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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
allele of DFR associated with red color, under the control of a doubled CaMV 35S promoter and tobacco etch virus translational enhancer introduced into the potato cultivar Prince Hairy (genotype dddd rrrr P-), which has white tubers and pale blue flowers
DFR2, DFR3 and DFR5 were expressed in Escherichia coli
-
enzyme expression analysis under UV-light irradiation, overview
-
Escherichia coli strains transformed with plasmid pTrcHis2-DFR to only express DFR. Escherichia coli strains harboring plasmid pET-DFR-LAR expressing DFR and leucoanthocyanidin reductase
-
expression in Escherichia coli
expression in Escherichia coli JM109
expression in Escherichia coli; into expression vector pQE-30 UA, and transformed into Escherichia coli M15 pREP-4 competent cells
expression in Nicotiana tabacum; expression in Nicotiana tabacum
expression in Saccharomyces cerevisiae; gene SmDFR, DNA and amino acid sequence determination and analysis, phylogenetic analysis, quantitative real-time PCR expression analysis, functional expression in Saccharomyces cerevisiae strain INV Sc1
functional expression in tobacco protoplasts via electroporation, subcloning and overexpression in Escherichia coli strain GI724 and in Saccharomyces cerevisiae strain INV Sc1, the recombinant Escherichia coli strain shows no enzyme activity
into pTrcHis2-TOPO and heterologously expressed in Escherichia coli TOP10F' strain. DFR cDNA cloned into pRSF-FHT and inserted into Escherichia coli BL21Star to create E-color strain
isozyme MtDFR1, from a young seed cDNA library, functional expression in Escherichia coli; isozyme MtDFR2, from a young seed cDNA library, functional expression in Escherichia coli
overexpression in Escherichia coli strain GI724 and in Saccharomyces cerevisiae strain INV Sc1
vector pC1-DFR overexpressed in transgenic tobacco plants, having distinctive petal colors, showing some variation in their intensity