Cloned (Comment) | Organism |
---|---|
into pTrcHis2-TOPO and heterologously expressed in Escherichia coli TOP10F' strain. DFR cDNA cloned into pRSF-FHT and inserted into Escherichia coli BL21Star to create E-color strain | Rosa hybrid cultivar |
into pTrcHis2-TOPO and heterologously expressed in Escherichia coli TOP10F' strain. DFR cDNA cloned into pRSF-FHT and inserted into Escherichia coli BL21Star to create E-color strain | Arabidopsis thaliana |
into pTrcHis2-TOPO and heterologously expressed in Escherichia coli TOP10F' strain. DFR cDNA cloned into pRSF-FHT and inserted into Escherichia coli BL21Star to create E-color strain | Anthurium andraeanum |
into pTrcHis2-TOPO and heterologously expressed in Escherichia coli TOP10F' strain. DFR cDNA cloned into pRSF-FHT and inserted into Escherichia coli BL21Star to create E-color strain | Lilium sp. |
into pTrcHis2-TOPO and heterologously expressed in Escherichia coli TOP10F' strain. DFR cDNA cloned into pRSF-FHT and inserted into Escherichia coli BL21Star to create E-color strain | Fragaria x ananassa |
into pTrcHis2-TOPO and heterologously expressed in Escherichia coli TOP10F' strain. DFR cDNA cloned into pRSF-FHT and inserted into Escherichia coli BL21Star to create E-color strain | Ipomoea nil |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Anthurium andraeanum | Q84L22 | - |
- |
Arabidopsis thaliana | P51102 | - |
- |
Fragaria x ananassa | O22617 | - |
- |
Ipomoea nil | O24607 | - |
- |
Lilium sp. | Q6UAQ7 | hybrid, cv. Star Gazer | - |
Rosa hybrid cultivar | Q41158 | cv. minirose | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | - |
Rosa hybrid cultivar | - |
flower | - |
Lilium sp. | - |
fruit | - |
Fragaria x ananassa | - |
additional information | spathe | Anthurium andraeanum | - |
petal | - |
Rosa hybrid cultivar | - |
petal | - |
Lilium sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00002 | - |
with dihydrokaempferol as substrate | Arabidopsis thaliana |
0.0003 | - |
with dihydrokaempferol as substrate | Ipomoea nil |
0.0003 | - |
with naringenin as substrate | Ipomoea nil |
0.0004 | - |
with dihydrokaempferol as substrate | Rosa hybrid cultivar |
0.0004 | - |
with naringenin as substrate | Rosa hybrid cultivar |
0.0005 | - |
with naringenin as substrate | Fragaria x ananassa |
0.0006 | - |
with eriodictyol as substrate | Arabidopsis thaliana |
0.0006 | - |
with eriodictyol as substrate | Lilium sp. |
0.0006 | - |
with eriodictyol as substrate | Fragaria x ananassa |
0.0006 | - |
with eriodictyol as substrate | Ipomoea nil |
0.0007 | - |
with eriodictyol as substrate | Rosa hybrid cultivar |
0.0007 | - |
with naringenin as substrate | Anthurium andraeanum |
0.0008 | - |
with eriodictyol as substrate | Anthurium andraeanum |
0.001 | - |
with dihydromyricetin as substrate | Arabidopsis thaliana |
0.001 | - |
with dihydroquercitin as substrate | Rosa hybrid cultivar |
0.0012 | - |
with dihydromyricetin as substrate | Lilium sp. |
0.0012 | - |
with dihydroquercitin as substrate | Fragaria x ananassa |
0.0013 | - |
with dihydromyricetin as substrate | Rosa hybrid cultivar |
0.0013 | - |
with dihydroquercitin as substrate | Arabidopsis thaliana |
0.0013 | - |
with dihydroquercitin as substrate | Lilium sp. |
0.0013 | - |
with dihydroquercitin as substrate | Ipomoea nil |
0.0014 | - |
with dihydromyricetin as substrate | Anthurium andraeanum |
0.0014 | - |
with dihydromyricetin as substrate | Fragaria x ananassa |
0.0014 | - |
with dihydroquercitin as substrate | Anthurium andraeanum |
0.0015 | - |
with dihydromyricetin as substrate | Ipomoea nil |
0.014 | - |
with dihydrokaempferol as substrate | Fragaria x ananassa |
0.021 | - |
with dihydrokaempferol as substrate | Anthurium andraeanum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7-hydroxyflavanone + NADPH + H+ | - |
Anthurium andraeanum | 2,4-cis-7-hydroxyflavan-4-ol + 2,4-trans-7-hydroxyflavan-4-ol + NADP+ | - |
? | |
dihydrokaempferol + NADPH + H+ | - |
Rosa hybrid cultivar | leucopelargonidin + NADP+ | - |
? | |
dihydrokaempferol + NADPH + H+ | - |
Arabidopsis thaliana | leucopelargonidin + NADP+ | - |
? | |
dihydrokaempferol + NADPH + H+ | - |
Fragaria x ananassa | leucopelargonidin + NADP+ | - |
? | |
dihydrokaempferol + NADPH + H+ | - |
Ipomoea nil | leucopelargonidin + NADP+ | - |
? | |
dihydrokaempferol + NADPH + H+ | - |
Anthurium andraeanum | leucopelargonidin + NADP+ | highest pelargonidin concentration derived from the E-color culture harboring the Anthurium andraeanum DFR | ? | |
dihydromyricetin + NADPH + H+ | - |
Rosa hybrid cultivar | leucodelphinidin + NADP+ | - |
? | |
dihydromyricetin + NADPH + H+ | - |
Arabidopsis thaliana | leucodelphinidin + NADP+ | - |
? | |
dihydromyricetin + NADPH + H+ | - |
Anthurium andraeanum | leucodelphinidin + NADP+ | - |
? | |
dihydromyricetin + NADPH + H+ | - |
Lilium sp. | leucodelphinidin + NADP+ | - |
? | |
dihydromyricetin + NADPH + H+ | - |
Fragaria x ananassa | leucodelphinidin + NADP+ | - |
? | |
dihydromyricetin + NADPH + H+ | - |
Ipomoea nil | leucodelphinidin + NADP+ | - |
? | |
dihydroquercetin + NADPH + H+ | - |
Rosa hybrid cultivar | leucocyanidin + NADP+ | - |
? | |
dihydroquercetin + NADPH + H+ | - |
Arabidopsis thaliana | leucocyanidin + NADP+ | - |
? | |
dihydroquercetin + NADPH + H+ | - |
Anthurium andraeanum | leucocyanidin + NADP+ | - |
? | |
dihydroquercetin + NADPH + H+ | - |
Lilium sp. | leucocyanidin + NADP+ | - |
? | |
dihydroquercetin + NADPH + H+ | - |
Fragaria x ananassa | leucocyanidin + NADP+ | - |
? | |
dihydroquercetin + NADPH + H+ | - |
Ipomoea nil | leucocyanidin + NADP+ | - |
? | |
eriodictyol + NADPH + H+ | - |
Rosa hybrid cultivar | luteoforol + NADP+ | - |
? | |
eriodictyol + NADPH + H+ | - |
Anthurium andraeanum | luteoforol + NADP+ | - |
? | |
eriodictyol + NADPH + H+ | - |
Lilium sp. | luteoforol + NADP+ | - |
? | |
eriodictyol + NADPH + H+ | - |
Fragaria x ananassa | luteoforol + NADP+ | - |
? | |
eriodictyol + NADPH + H+ | - |
Ipomoea nil | luteoforol + NADP+ | - |
? | |
additional information | can not reduce hesperetin or 5,7-dimethoxyflavanone | Anthurium andraeanum | ? | - |
? | |
additional information | does not catalyze dihydrokaempferol and naringenin | Lilium sp. | ? | - |
? | |
additional information | does not catalyze naringenin | Arabidopsis thaliana | ? | - |
? | |
naringenin + NADPH + H+ | - |
Rosa hybrid cultivar | apiferol + NADP+ | - |
? | |
naringenin + NADPH + H+ | - |
Anthurium andraeanum | apiferol + NADP+ | - |
? | |
naringenin + NADPH + H+ | - |
Fragaria x ananassa | apiferol + NADP+ | - |
? | |
naringenin + NADPH + H+ | - |
Ipomoea nil | apiferol + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DFR | - |
Rosa hybrid cultivar |
DFR | - |
Arabidopsis thaliana |
DFR | - |
Anthurium andraeanum |
DFR | - |
Lilium sp. |
DFR | - |
Fragaria x ananassa |
DFR | - |
Ipomoea nil |
dihydroflavonol 4-reductase | - |
Rosa hybrid cultivar |
dihydroflavonol 4-reductase | - |
Arabidopsis thaliana |
dihydroflavonol 4-reductase | - |
Anthurium andraeanum |
dihydroflavonol 4-reductase | - |
Lilium sp. |
dihydroflavonol 4-reductase | - |
Fragaria x ananassa |
dihydroflavonol 4-reductase | - |
Ipomoea nil |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 30 | maximum DFR activities achieved towards dihydroflavonols and flavanones | Anthurium andraeanum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 7 | maximum DFR activities achieved towards dihydroflavonols and flavanones | Anthurium andraeanum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Rosa hybrid cultivar | |
NADPH | - |
Arabidopsis thaliana | |
NADPH | - |
Anthurium andraeanum | |
NADPH | - |
Lilium sp. | |
NADPH | - |
Fragaria x ananassa | |
NADPH | - |
Ipomoea nil |
General Information | Comment | Organism |
---|---|---|
physiological function | DFR plays a key role in determining intensity and pigment coloration because its specificity and activities dictate the type and amount of the colorless leucoanthocyanidins | Rosa hybrid cultivar |
physiological function | DFR plays a key role in determining intensity and pigment coloration because its specificity and activities dictate the type and amount of the colorless leucoanthocyanidins | Arabidopsis thaliana |
physiological function | DFR plays a key role in determining intensity and pigment coloration because its specificity and activities dictate the type and amount of the colorless leucoanthocyanidins | Anthurium andraeanum |
physiological function | DFR plays a key role in determining intensity and pigment coloration because its specificity and activities dictate the type and amount of the colorless leucoanthocyanidins | Lilium sp. |
physiological function | DFR plays a key role in determining intensity and pigment coloration because its specificity and activities dictate the type and amount of the colorless leucoanthocyanidins | Fragaria x ananassa |
physiological function | DFR plays a key role in determining intensity and pigment coloration because its specificity and activities dictate the type and amount of the colorless leucoanthocyanidins | Ipomoea nil |