BRENDA - Enzyme Database show
show all sequences of 1.1.1.219

Characterization of dihydroflavonol 4-reductases for recombinant plant pigment biosynthesis applications

Leonard, E.; Yan, Y.; Chemler, J.; Matern, U.; Martens, S.; Koffas, M.; Biocatal. Biotransform. 26, 243-251 (2008)
No PubMed abstract available

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
into pTrcHis2-TOPO and heterologously expressed in Escherichia coli TOP10F' strain. DFR cDNA cloned into pRSF-FHT and inserted into Escherichia coli BL21Star to create E-color strain
Anthurium andraeanum
into pTrcHis2-TOPO and heterologously expressed in Escherichia coli TOP10F' strain. DFR cDNA cloned into pRSF-FHT and inserted into Escherichia coli BL21Star to create E-color strain
Arabidopsis thaliana
into pTrcHis2-TOPO and heterologously expressed in Escherichia coli TOP10F' strain. DFR cDNA cloned into pRSF-FHT and inserted into Escherichia coli BL21Star to create E-color strain
Fragaria x ananassa
into pTrcHis2-TOPO and heterologously expressed in Escherichia coli TOP10F' strain. DFR cDNA cloned into pRSF-FHT and inserted into Escherichia coli BL21Star to create E-color strain
Ipomoea nil
into pTrcHis2-TOPO and heterologously expressed in Escherichia coli TOP10F' strain. DFR cDNA cloned into pRSF-FHT and inserted into Escherichia coli BL21Star to create E-color strain
Lilium sp.
into pTrcHis2-TOPO and heterologously expressed in Escherichia coli TOP10F' strain. DFR cDNA cloned into pRSF-FHT and inserted into Escherichia coli BL21Star to create E-color strain
Rosa hybrid cultivar
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Anthurium andraeanum
Q84L22
-
-
Arabidopsis thaliana
P51102
-
-
Fragaria x ananassa
O22617
-
-
Ipomoea nil
O24607
-
-
Lilium sp.
Q6UAQ7
hybrid, cv. Star Gazer
-
Rosa hybrid cultivar
Q41158
cv. minirose
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
flower
-
Lilium sp.
-
flower
-
Rosa hybrid cultivar
-
fruit
-
Fragaria x ananassa
-
additional information
spathe
Anthurium andraeanum
-
petal
-
Lilium sp.
-
petal
-
Rosa hybrid cultivar
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.00002
-
with dihydrokaempferol as substrate
Arabidopsis thaliana
0.0003
-
with dihydrokaempferol as substrate; with naringenin as substrate
Ipomoea nil
0.0004
-
with dihydrokaempferol as substrate; with naringenin as substrate
Rosa hybrid cultivar
0.0005
-
with naringenin as substrate
Fragaria x ananassa
0.0006
-
with eriodictyol as substrate
Arabidopsis thaliana
0.0006
-
with eriodictyol as substrate
Fragaria x ananassa
0.0006
-
with eriodictyol as substrate
Ipomoea nil
0.0006
-
with eriodictyol as substrate
Lilium sp.
0.0007
-
with naringenin as substrate
Anthurium andraeanum
0.0007
-
with eriodictyol as substrate
Rosa hybrid cultivar
0.0008
-
with eriodictyol as substrate
Anthurium andraeanum
0.001
-
with dihydromyricetin as substrate
Arabidopsis thaliana
0.001
-
with dihydroquercitin as substrate
Rosa hybrid cultivar
0.0012
-
with dihydroquercitin as substrate
Fragaria x ananassa
0.0012
-
with dihydromyricetin as substrate
Lilium sp.
0.0013
-
with dihydroquercitin as substrate
Arabidopsis thaliana
0.0013
-
with dihydroquercitin as substrate
Ipomoea nil
0.0013
-
with dihydroquercitin as substrate
Lilium sp.
0.0013
-
with dihydromyricetin as substrate
Rosa hybrid cultivar
0.0014
-
with dihydromyricetin as substrate; with dihydroquercitin as substrate
Anthurium andraeanum
0.0014
-
with dihydromyricetin as substrate
Fragaria x ananassa
0.0015
-
with dihydromyricetin as substrate
Ipomoea nil
0.014
-
with dihydrokaempferol as substrate
Fragaria x ananassa
0.021
-
with dihydrokaempferol as substrate
Anthurium andraeanum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
7-hydroxyflavanone + NADPH + H+
-
702000
Anthurium andraeanum
2,4-cis-7-hydroxyflavan-4-ol + 2,4-trans-7-hydroxyflavan-4-ol + NADP+
-
-
-
?
dihydrokaempferol + NADPH + H+
-
702000
Rosa hybrid cultivar
leucopelargonidin + NADP+
-
-
-
?
dihydrokaempferol + NADPH + H+
-
702000
Arabidopsis thaliana
leucopelargonidin + NADP+
-
-
-
?
dihydrokaempferol + NADPH + H+
-
702000
Fragaria x ananassa
leucopelargonidin + NADP+
-
-
-
?
dihydrokaempferol + NADPH + H+
-
702000
Ipomoea nil
leucopelargonidin + NADP+
-
-
-
?
dihydrokaempferol + NADPH + H+
-
702000
Anthurium andraeanum
leucopelargonidin + NADP+
highest pelargonidin concentration derived from the E-color culture harboring the Anthurium andraeanum DFR
-
-
?
dihydromyricetin + NADPH + H+
-
702000
Rosa hybrid cultivar
leucodelphinidin + NADP+
-
-
-
?
dihydromyricetin + NADPH + H+
-
702000
Arabidopsis thaliana
leucodelphinidin + NADP+
-
-
-
?
dihydromyricetin + NADPH + H+
-
702000
Anthurium andraeanum
leucodelphinidin + NADP+
-
-
-
?
dihydromyricetin + NADPH + H+
-
702000
Lilium sp.
leucodelphinidin + NADP+
-
-
-
?
dihydromyricetin + NADPH + H+
-
702000
Fragaria x ananassa
leucodelphinidin + NADP+
-
-
-
?
dihydromyricetin + NADPH + H+
-
702000
Ipomoea nil
leucodelphinidin + NADP+
-
-
-
?
dihydroquercetin + NADPH + H+
-
702000
Rosa hybrid cultivar
leucocyanidin + NADP+
-
-
-
?
dihydroquercetin + NADPH + H+
-
702000
Arabidopsis thaliana
leucocyanidin + NADP+
-
-
-
?
dihydroquercetin + NADPH + H+
-
702000
Anthurium andraeanum
leucocyanidin + NADP+
-
-
-
?
dihydroquercetin + NADPH + H+
-
702000
Lilium sp.
leucocyanidin + NADP+
-
-
-
?
dihydroquercetin + NADPH + H+
-
702000
Fragaria x ananassa
leucocyanidin + NADP+
-
-
-
?
dihydroquercetin + NADPH + H+
-
702000
Ipomoea nil
leucocyanidin + NADP+
-
-
-
?
eriodictyol + NADPH + H+
-
702000
Rosa hybrid cultivar
luteoforol + NADP+
-
-
-
?
eriodictyol + NADPH + H+
-
702000
Anthurium andraeanum
luteoforol + NADP+
-
-
-
?
eriodictyol + NADPH + H+
-
702000
Lilium sp.
luteoforol + NADP+
-
-
-
?
eriodictyol + NADPH + H+
-
702000
Fragaria x ananassa
luteoforol + NADP+
-
-
-
?
eriodictyol + NADPH + H+
-
702000
Ipomoea nil
luteoforol + NADP+
-
-
-
?
additional information
can not reduce hesperetin or 5,7-dimethoxyflavanone
702000
Anthurium andraeanum
?
-
-
-
-
additional information
does not catalyze dihydrokaempferol and naringenin
702000
Lilium sp.
?
-
-
-
-
additional information
does not catalyze naringenin
702000
Arabidopsis thaliana
?
-
-
-
-
naringenin + NADPH + H+
-
702000
Rosa hybrid cultivar
apiferol + NADP+
-
-
-
?
naringenin + NADPH + H+
-
702000
Anthurium andraeanum
apiferol + NADP+
-
-
-
?
naringenin + NADPH + H+
-
702000
Fragaria x ananassa
apiferol + NADP+
-
-
-
?
naringenin + NADPH + H+
-
702000
Ipomoea nil
apiferol + NADP+
-
-
-
?
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
25
30
maximum DFR activities achieved towards dihydroflavonols and flavanones
Anthurium andraeanum
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6
7
maximum DFR activities achieved towards dihydroflavonols and flavanones
Anthurium andraeanum
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Anthurium andraeanum
NADPH
-
Arabidopsis thaliana
NADPH
-
Fragaria x ananassa
NADPH
-
Ipomoea nil
NADPH
-
Lilium sp.
NADPH
-
Rosa hybrid cultivar
Cloned(Commentary) (protein specific)
Commentary
Organism
into pTrcHis2-TOPO and heterologously expressed in Escherichia coli TOP10F' strain. DFR cDNA cloned into pRSF-FHT and inserted into Escherichia coli BL21Star to create E-color strain
Anthurium andraeanum
into pTrcHis2-TOPO and heterologously expressed in Escherichia coli TOP10F' strain. DFR cDNA cloned into pRSF-FHT and inserted into Escherichia coli BL21Star to create E-color strain
Arabidopsis thaliana
into pTrcHis2-TOPO and heterologously expressed in Escherichia coli TOP10F' strain. DFR cDNA cloned into pRSF-FHT and inserted into Escherichia coli BL21Star to create E-color strain
Fragaria x ananassa
into pTrcHis2-TOPO and heterologously expressed in Escherichia coli TOP10F' strain. DFR cDNA cloned into pRSF-FHT and inserted into Escherichia coli BL21Star to create E-color strain
Ipomoea nil
into pTrcHis2-TOPO and heterologously expressed in Escherichia coli TOP10F' strain. DFR cDNA cloned into pRSF-FHT and inserted into Escherichia coli BL21Star to create E-color strain
Lilium sp.
into pTrcHis2-TOPO and heterologously expressed in Escherichia coli TOP10F' strain. DFR cDNA cloned into pRSF-FHT and inserted into Escherichia coli BL21Star to create E-color strain
Rosa hybrid cultivar
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Anthurium andraeanum
NADPH
-
Arabidopsis thaliana
NADPH
-
Fragaria x ananassa
NADPH
-
Ipomoea nil
NADPH
-
Lilium sp.
NADPH
-
Rosa hybrid cultivar
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
flower
-
Lilium sp.
-
flower
-
Rosa hybrid cultivar
-
fruit
-
Fragaria x ananassa
-
additional information
spathe
Anthurium andraeanum
-
petal
-
Lilium sp.
-
petal
-
Rosa hybrid cultivar
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.00002
-
with dihydrokaempferol as substrate
Arabidopsis thaliana
0.0003
-
with dihydrokaempferol as substrate; with naringenin as substrate
Ipomoea nil
0.0004
-
with dihydrokaempferol as substrate; with naringenin as substrate
Rosa hybrid cultivar
0.0005
-
with naringenin as substrate
Fragaria x ananassa
0.0006
-
with eriodictyol as substrate
Arabidopsis thaliana
0.0006
-
with eriodictyol as substrate
Fragaria x ananassa
0.0006
-
with eriodictyol as substrate
Ipomoea nil
0.0006
-
with eriodictyol as substrate
Lilium sp.
0.0007
-
with naringenin as substrate
Anthurium andraeanum
0.0007
-
with eriodictyol as substrate
Rosa hybrid cultivar
0.0008
-
with eriodictyol as substrate
Anthurium andraeanum
0.001
-
with dihydromyricetin as substrate
Arabidopsis thaliana
0.001
-
with dihydroquercitin as substrate
Rosa hybrid cultivar
0.0012
-
with dihydroquercitin as substrate
Fragaria x ananassa
0.0012
-
with dihydromyricetin as substrate
Lilium sp.
0.0013
-
with dihydroquercitin as substrate
Arabidopsis thaliana
0.0013
-
with dihydroquercitin as substrate
Ipomoea nil
0.0013
-
with dihydroquercitin as substrate
Lilium sp.
0.0013
-
with dihydromyricetin as substrate
Rosa hybrid cultivar
0.0014
-
with dihydromyricetin as substrate; with dihydroquercitin as substrate
Anthurium andraeanum
0.0014
-
with dihydromyricetin as substrate
Fragaria x ananassa
0.0015
-
with dihydromyricetin as substrate
Ipomoea nil
0.014
-
with dihydrokaempferol as substrate
Fragaria x ananassa
0.021
-
with dihydrokaempferol as substrate
Anthurium andraeanum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
7-hydroxyflavanone + NADPH + H+
-
702000
Anthurium andraeanum
2,4-cis-7-hydroxyflavan-4-ol + 2,4-trans-7-hydroxyflavan-4-ol + NADP+
-
-
-
?
dihydrokaempferol + NADPH + H+
-
702000
Rosa hybrid cultivar
leucopelargonidin + NADP+
-
-
-
?
dihydrokaempferol + NADPH + H+
-
702000
Arabidopsis thaliana
leucopelargonidin + NADP+
-
-
-
?
dihydrokaempferol + NADPH + H+
-
702000
Fragaria x ananassa
leucopelargonidin + NADP+
-
-
-
?
dihydrokaempferol + NADPH + H+
-
702000
Ipomoea nil
leucopelargonidin + NADP+
-
-
-
?
dihydrokaempferol + NADPH + H+
-
702000
Anthurium andraeanum
leucopelargonidin + NADP+
highest pelargonidin concentration derived from the E-color culture harboring the Anthurium andraeanum DFR
-
-
?
dihydromyricetin + NADPH + H+
-
702000
Rosa hybrid cultivar
leucodelphinidin + NADP+
-
-
-
?
dihydromyricetin + NADPH + H+
-
702000
Arabidopsis thaliana
leucodelphinidin + NADP+
-
-
-
?
dihydromyricetin + NADPH + H+
-
702000
Anthurium andraeanum
leucodelphinidin + NADP+
-
-
-
?
dihydromyricetin + NADPH + H+
-
702000
Lilium sp.
leucodelphinidin + NADP+
-
-
-
?
dihydromyricetin + NADPH + H+
-
702000
Fragaria x ananassa
leucodelphinidin + NADP+
-
-
-
?
dihydromyricetin + NADPH + H+
-
702000
Ipomoea nil
leucodelphinidin + NADP+
-
-
-
?
dihydroquercetin + NADPH + H+
-
702000
Rosa hybrid cultivar
leucocyanidin + NADP+
-
-
-
?
dihydroquercetin + NADPH + H+
-
702000
Arabidopsis thaliana
leucocyanidin + NADP+
-
-
-
?
dihydroquercetin + NADPH + H+
-
702000
Anthurium andraeanum
leucocyanidin + NADP+
-
-
-
?
dihydroquercetin + NADPH + H+
-
702000
Lilium sp.
leucocyanidin + NADP+
-
-
-
?
dihydroquercetin + NADPH + H+
-
702000
Fragaria x ananassa
leucocyanidin + NADP+
-
-
-
?
dihydroquercetin + NADPH + H+
-
702000
Ipomoea nil
leucocyanidin + NADP+
-
-
-
?
eriodictyol + NADPH + H+
-
702000
Rosa hybrid cultivar
luteoforol + NADP+
-
-
-
?
eriodictyol + NADPH + H+
-
702000
Anthurium andraeanum
luteoforol + NADP+
-
-
-
?
eriodictyol + NADPH + H+
-
702000
Lilium sp.
luteoforol + NADP+
-
-
-
?
eriodictyol + NADPH + H+
-
702000
Fragaria x ananassa
luteoforol + NADP+
-
-
-
?
eriodictyol + NADPH + H+
-
702000
Ipomoea nil
luteoforol + NADP+
-
-
-
?
additional information
can not reduce hesperetin or 5,7-dimethoxyflavanone
702000
Anthurium andraeanum
?
-
-
-
-
additional information
does not catalyze dihydrokaempferol and naringenin
702000
Lilium sp.
?
-
-
-
-
additional information
does not catalyze naringenin
702000
Arabidopsis thaliana
?
-
-
-
-
naringenin + NADPH + H+
-
702000
Rosa hybrid cultivar
apiferol + NADP+
-
-
-
?
naringenin + NADPH + H+
-
702000
Anthurium andraeanum
apiferol + NADP+
-
-
-
?
naringenin + NADPH + H+
-
702000
Fragaria x ananassa
apiferol + NADP+
-
-
-
?
naringenin + NADPH + H+
-
702000
Ipomoea nil
apiferol + NADP+
-
-
-
?
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
25
30
maximum DFR activities achieved towards dihydroflavonols and flavanones
Anthurium andraeanum
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6
7
maximum DFR activities achieved towards dihydroflavonols and flavanones
Anthurium andraeanum
General Information
General Information
Commentary
Organism
physiological function
DFR plays a key role in determining intensity and pigment coloration because its specificity and activities dictate the type and amount of the colorless leucoanthocyanidins
Anthurium andraeanum
physiological function
DFR plays a key role in determining intensity and pigment coloration because its specificity and activities dictate the type and amount of the colorless leucoanthocyanidins
Arabidopsis thaliana
physiological function
DFR plays a key role in determining intensity and pigment coloration because its specificity and activities dictate the type and amount of the colorless leucoanthocyanidins
Fragaria x ananassa
physiological function
DFR plays a key role in determining intensity and pigment coloration because its specificity and activities dictate the type and amount of the colorless leucoanthocyanidins
Ipomoea nil
physiological function
DFR plays a key role in determining intensity and pigment coloration because its specificity and activities dictate the type and amount of the colorless leucoanthocyanidins
Lilium sp.
physiological function
DFR plays a key role in determining intensity and pigment coloration because its specificity and activities dictate the type and amount of the colorless leucoanthocyanidins
Rosa hybrid cultivar
General Information (protein specific)
General Information
Commentary
Organism
physiological function
DFR plays a key role in determining intensity and pigment coloration because its specificity and activities dictate the type and amount of the colorless leucoanthocyanidins
Anthurium andraeanum
physiological function
DFR plays a key role in determining intensity and pigment coloration because its specificity and activities dictate the type and amount of the colorless leucoanthocyanidins
Arabidopsis thaliana
physiological function
DFR plays a key role in determining intensity and pigment coloration because its specificity and activities dictate the type and amount of the colorless leucoanthocyanidins
Fragaria x ananassa
physiological function
DFR plays a key role in determining intensity and pigment coloration because its specificity and activities dictate the type and amount of the colorless leucoanthocyanidins
Ipomoea nil
physiological function
DFR plays a key role in determining intensity and pigment coloration because its specificity and activities dictate the type and amount of the colorless leucoanthocyanidins
Lilium sp.
physiological function
DFR plays a key role in determining intensity and pigment coloration because its specificity and activities dictate the type and amount of the colorless leucoanthocyanidins
Rosa hybrid cultivar
Other publictions for EC 1.1.1.219
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
738059
Bulut
Screening, molecular cloning, ...
Komagataella phaffii, Komagataella phaffii GS115
ChemBioChem
17
1349-1358
2016
-
-
1
-
1
-
-
3
-
-
-
-
-
2
-
-
-
-
-
-
-
1
15
1
1
-
1
3
1
1
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
1
15
1
1
-
1
3
1
1
-
-
-
-
-
-
-
-
725929
Li
Molecular characterization and ...
Saussurea medusa
Mol. Biol. Rep.
39
2991-2999
2012
-
-
1
-
-
-
-
-
-
-
1
1
-
6
-
-
-
-
-
5
-
-
6
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
5
-
-
6
1
-
-
-
-
-
-
-
-
-
3
3
-
-
-
726292
Huang
Molecular cloning and characte ...
Populus trichocarpa
PLoS ONE
7
e30364
2012
-
-
1
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
1
2
-
-
-
704148
Cao
Effect of BTH on anthocyanin c ...
Fragaria x ananassa
J. Agric. Food Chem.
58
5801-5805
2010
1
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
1
1
1
-
1
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
1
1
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
700794
Eungwanichayapant
Accumulation of catechins in t ...
Camellia sinensis var. sinensis
Plant Physiol. Biochem.
47
94-97
2009
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
702936
Akhov
-
Proanthocyanidin biosynthesis ...
Brassica napus
Botany
87
616-625
2009
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
705520
Chemler
Improving NADPH availability f ...
Anthurium andraeanum
Metab. Eng.
12
96-104
2009
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
706155
Singh
-
Characterization of dihydrofla ...
Camellia sinensis, Camellia sinensis (L.) O. Kuntze
Plant Biotechnol. Rep.
3
95-101
2009
-
-
1
-
-
-
2
-
1
-
2
-
-
3
-
-
1
-
-
1
2
-
3
1
-
-
-
-
-
-
-
1
-
2
-
-
-
2
1
-
-
-
-
2
-
-
2
-
4
-
-
-
-
1
-
2
2
-
3
2
-
-
-
-
-
-
-
2
2
-
-
4
-
-
706810
Zhang
The potato R locus codes for d ...
Solanum tuberosum
Theor. Appl. Genet.
119
931-937
2009
-
-
1
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
713265
Yong
-
Agrobacterium-mediated transfo ...
Torenia fournieri
Plant Cell Tissue Organ Cult.
96
59-67
2009
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
713530
Jung
The potato developer (D) locus ...
Solanum tuberosum
Theor. Appl. Genet.
120
45-57
2009
-
1
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
701213
Nakatsuka
-
Isolation and expression analy ...
Rhododendron x pulchrum
Sci. Hortic.
118
314-320
2008
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
1
1
1
1
-
-
701463
Trabelsi
Structural evidence for the in ...
Vitis vinifera
Acta Crystallogr. Sect. D
64
883-891
2008
-
-
-
1
-
-
2
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
1
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
702000
Leonard
-
Characterization of dihydrofla ...
Anthurium andraeanum, Arabidopsis thaliana, Fragaria x ananassa, Ipomoea nil, Lilium sp., Rosa hybrid cultivar
Biocatal. Biotransform.
26
243-251
2008
-
-
6
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
6
24
-
30
-
-
1
-
-
-
1
-
6
-
-
-
-
-
6
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
24
-
30
-
-
1
-
-
-
1
-
-
-
6
6
-
-
-
705358
Lee
-
Molecular cloning and biochemi ...
Brassica rapa subsp. pekinensis
J. Plant Biol.
51
42-47
2008
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
688073
Zhou
Ultraviolet A-specific inducti ...
Brassica rapa
J. Exp. Bot.
58
1771-1781
2007
1
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
688348
Petit
Crystal structure of grape dih ...
Vitis vinifera
J. Mol. Biol.
368
1345-1357
2007
-
-
1
1
1
-
-
-
-
-
-
4
-
5
-
-
1
-
-
-
-
-
5
1
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
1
1
-
-
-
-
-
-
-
-
4
-
-
-
1
-
-
-
-
5
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
667201
Takahashi
Evaluation of metabolic altera ...
Oryza sativa
Ann. Bot.
98
819-825
2006
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
670520
Lo Piero
Gene characterization, analysi ...
Citrus sinensis
Phytochemistry
67
684-695
2006
-
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
1
-
-
1
2
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
1
2
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
669664
Shimada
A comprehensive analysis of si ...
Lotus japonicus
J. Exp. Bot.
56
2573-2585
2005
-
-
1
-
-
-
-
-
-
-
5
-
-
4
-
-
-
-
-
1
-
-
5
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
1
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
670686
Hayashi
Enhanced dihydroflavonol-4-red ...
Oryza sativa
Proc. Natl. Acad. Sci. USA
102
7020-7025
2005
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
2
6
-
1
-
1
-
-
-
1
1
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
6
-
1
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-
670928
Kim
Development of a PCR-based mar ...
Allium cepa
Theor. Appl. Genet.
110
588-595
2005
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
657067
Xie
Molecular and biochemical anal ...
Medicago truncatula
Plant Physiol.
134
979-994
2004
-
-
1
-
1
-
-
-
-
-
-
1
-
7
-
-
-
-
-
4
-
-
9
-
1
-
-
-
2
-
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
4
-
-
9
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
655562
Martens
Heterologous expression of dih ...
Callistephus chinensis, Dianthus caryophyllus, Gerbera hybrid cultivar, Matthiola incana, no activity in Escherichia coli, no activity in Nicotiana tabacum, no activity in Saccharomyces cerevisiae, Rosa hybrid cultivar, Rosa hybrid cultivar Kardinal, Solanum lycopersicum
FEBS Lett.
531
453-458
2002
-
-
6
-
-
-
-
-
-
-
-
7
-
10
-
-
-
-
-
18
-
-
29
-
6
-
-
-
6
-
-
6
-
-
-
-
-
6
6
-
-
-
-
-
-
-
-
-
-
7
-
-
-
-
-
18
-
-
29
-
6
-
-
-
6
-
-
-
-
-
-
-
-
-
348000
Singh
-
Biosynthesis of flavan-3-ols b ...
Onobrychis viciifolia
Phytochemistry
44
425-432
1997
-
-
-
-
-
-
2
-
-
-
-
1
-
1
-
-
-
-
-
1
1
1
4
-
1
-
-
-
-
1
-
2
-
-
-
-
-
-
2
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
1
1
1
4
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
347998
Ishikura
-
Conversion of (+)-dihydroquerc ...
Cryptomeria japonica
Plant Cell Physiol.
29
795-799
1988
-
-
-
-
-
-
3
1
-
-
1
-
-
1
-
-
1
-
-
2
1
2
3
-
1
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
3
-
1
-
-
1
-
-
-
-
1
-
2
1
2
3
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
347995
Stafford
Flavan-3-ol biosynthesis. The ...
Ginkgo biloba, Pseudotsuga menziesii
Plant Physiol.
78
791-794
1985
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
4
1
-
4
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
1
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
347996
Heller
Enzymatic reduction of (+)-dih ...
Matthiola incana
Planta
165
284-287
1985
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
1
1
1
4
-
1
-
-
-
1
1
-
3
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
1
4
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-
347999
Stafford
Flavan-3-ol biosynthesis. The ...
Pseudotsuga menziesii
Plant Physiol.
76
184-186
1984
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
347997
Stafford
Enzymic and nonenzymic reducti ...
Pseudotsuga menziesii
Plant Physiol.
70
695-698
1982
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-