BRENDA - Enzyme Database show
show all sequences of 1.1.1.219

Characterization of dihydroflavonol 4-reductase cDNA in tea [Camellia sinensis (L.) O. Kuntze]

Singh, K.; Kumar, S.; Yadav, S.; Ahuja, P.; Plant Biotechnol. Rep. 3, 95-101 (2009)
No PubMed abstract available

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli; into expression vector pQE-30 UA, and transformed into Escherichia coli M15 pREP-4 competent cells
Camellia sinensis
Inhibitors
Inhibitors
Commentary
Organism
Structure
abscisic acid
decreases expression
Camellia sinensis
additional information
is unaffected by gibberellic acid treatment
Camellia sinensis
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
;
Camellia sinensis
16020
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
38670
-
sequence analysis; x * 38670, calculated, x * 41000, SDS-PAGE of recombinant protein with fusion peptide
Camellia sinensis
41000
-
x * 38670, calculated, x * 41000, SDS-PAGE of recombinant protein with fusion peptide; x * 41000, SDS-PAGE
Camellia sinensis
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Camellia sinensis
Q6DT40
-
-
Camellia sinensis
Q9S787
-
-
Camellia sinensis (L.) O. Kuntze
Q6DT40
-
-
Purification (Commentary)
Commentary
Organism
on Ni-NTA column
Camellia sinensis
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
expression is maximal in younger rather than older leaves; expression is maximum in younger rather than older leaves
Camellia sinensis
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
42.8
-
pH 7.0, 25°C
Camellia sinensis
42850
-
recombinant protein
Camellia sinensis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(+)-dihydroquercetin + NADPH + H+
-
706155
Camellia sinensis
cis-3,4-leucocyanidin + NADP+
-
-
-
?
(+)-dihydroquercetin + NADPH + H+
-
706155
Camellia sinensis (L.) O. Kuntze
cis-3,4-leucocyanidin + NADP+
-
-
-
?
dihydroquercetin + NADPH + H+
-
706155
Camellia sinensis
leucocyanidin + NADP+
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 38670, calculated, x * 41000, SDS-PAGE of recombinant protein with fusion peptide; x * 41000, SDS-PAGE
Camellia sinensis
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Camellia sinensis
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Camellia sinensis
calculated
-
6.2
Camellia sinensis
sequence analysis
-
6.22
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Camellia sinensis
into expression vector pQE-30 UA, and transformed into Escherichia coli M15 pREP-4 competent cells
Camellia sinensis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Camellia sinensis
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
abscisic acid
decreases expression
Camellia sinensis
additional information
is unaffected by gibberellic acid treatment
Camellia sinensis
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Camellia sinensis
16020
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
38670
-
sequence analysis
Camellia sinensis
38670
-
x * 38670, calculated, x * 41000, SDS-PAGE of recombinant protein with fusion peptide
Camellia sinensis
41000
-
x * 41000, SDS-PAGE
Camellia sinensis
41000
-
x * 38670, calculated, x * 41000, SDS-PAGE of recombinant protein with fusion peptide
Camellia sinensis
Purification (Commentary) (protein specific)
Commentary
Organism
on Ni-NTA column
Camellia sinensis
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
expression is maximum in younger rather than older leaves
Camellia sinensis
-
leaf
expression is maximal in younger rather than older leaves
Camellia sinensis
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
42.8
-
pH 7.0, 25°C
Camellia sinensis
42850
-
recombinant protein
Camellia sinensis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(+)-dihydroquercetin + NADPH + H+
-
706155
Camellia sinensis
cis-3,4-leucocyanidin + NADP+
-
-
-
?
(+)-dihydroquercetin + NADPH + H+
-
706155
Camellia sinensis (L.) O. Kuntze
cis-3,4-leucocyanidin + NADP+
-
-
-
?
dihydroquercetin + NADPH + H+
-
706155
Camellia sinensis
leucocyanidin + NADP+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 41000, SDS-PAGE
Camellia sinensis
?
x * 38670, calculated, x * 41000, SDS-PAGE of recombinant protein with fusion peptide
Camellia sinensis
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Camellia sinensis
calculated
-
6.2
Camellia sinensis
sequence analysis
-
6.22
Expression
Organism
Commentary
Expression
Camellia sinensis
expression is down-regulated in response to drought stress and abscisic acid, and unaffected by gibberellic acid treatment; in response to drought stress
down
Camellia sinensis
expression is up-regulated in response to wounding, with concomitant modulation of catechins content; in response to wounding, with concomitant modulation of catechins content. Expression is maximum in younger rather than older leaves
up
Expression (protein specific)
Organism
Commentary
Expression
Camellia sinensis
in response to drought stress
down
Camellia sinensis
expression is down-regulated in response to drought stress and abscisic acid, and unaffected by gibberellic acid treatment
down
Camellia sinensis
in response to wounding, with concomitant modulation of catechins content. Expression is maximum in younger rather than older leaves
up
Camellia sinensis
expression is up-regulated in response to wounding, with concomitant modulation of catechins content
up
Other publictions for EC 1.1.1.219
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
738059
Bulut
Screening, molecular cloning, ...
Komagataella phaffii, Komagataella phaffii GS115
ChemBioChem
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1349-1358
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1
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15
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3
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1
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1
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1
15
1
1
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3
1
1
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725929
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Molecular characterization and ...
Saussurea medusa
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2991-2999
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1
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6
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1
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6
1
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3
3
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726292
Huang
Molecular cloning and characte ...
Populus trichocarpa
PLoS ONE
7
e30364
2012
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1
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6
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5
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1
2
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704148
Cao
Effect of BTH on anthocyanin c ...
Fragaria x ananassa
J. Agric. Food Chem.
58
5801-5805
2010
1
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2
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1
1
1
1
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1
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1
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1
1
-
-
-
700794
Eungwanichayapant
Accumulation of catechins in t ...
Camellia sinensis var. sinensis
Plant Physiol. Biochem.
47
94-97
2009
-
-
-
-
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2
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2
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2
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-
-
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1
1
-
-
-
702936
Akhov
-
Proanthocyanidin biosynthesis ...
Brassica napus
Botany
87
616-625
2009
-
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-
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-
-
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1
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1
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1
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1
1
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-
705520
Chemler
Improving NADPH availability f ...
Anthurium andraeanum
Metab. Eng.
12
96-104
2009
-
-
1
-
-
-
-
-
-
-
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2
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1
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1
1
-
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-
706155
Singh
-
Characterization of dihydrofla ...
Camellia sinensis, Camellia sinensis (L.) O. Kuntze
Plant Biotechnol. Rep.
3
95-101
2009
-
-
1
-
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2
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1
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2
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3
-
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1
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1
2
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3
1
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1
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2
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2
1
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2
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2
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4
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1
-
2
2
-
3
2
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-
2
2
-
-
4
-
-
706810
Zhang
The potato R locus codes for d ...
Solanum tuberosum
Theor. Appl. Genet.
119
931-937
2009
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1
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4
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1
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1
1
-
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-
713265
Yong
-
Agrobacterium-mediated transfo ...
Torenia fournieri
Plant Cell Tissue Organ Cult.
96
59-67
2009
-
1
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1
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713530
Jung
The potato developer (D) locus ...
Solanum tuberosum
Theor. Appl. Genet.
120
45-57
2009
-
1
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4
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1
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1
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1
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1
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1
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701213
Nakatsuka
-
Isolation and expression analy ...
Rhododendron x pulchrum
Sci. Hortic.
118
314-320
2008
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1
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4
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1
1
1
1
-
-
701463
Trabelsi
Structural evidence for the in ...
Vitis vinifera
Acta Crystallogr. Sect. D
64
883-891
2008
-
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1
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2
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4
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1
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702000
Leonard
-
Characterization of dihydrofla ...
Anthurium andraeanum, Arabidopsis thaliana, Fragaria x ananassa, Ipomoea nil, Lilium sp., Rosa hybrid cultivar
Biocatal. Biotransform.
26
243-251
2008
-
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6
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6
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6
24
-
30
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1
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1
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6
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6
6
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6
24
-
30
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1
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1
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6
6
-
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-
705358
Lee
-
Molecular cloning and biochemi ...
Brassica rapa subsp. pekinensis
J. Plant Biol.
51
42-47
2008
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1
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1
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688073
Zhou
Ultraviolet A-specific inducti ...
Brassica rapa
J. Exp. Bot.
58
1771-1781
2007
1
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1
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688348
Petit
Crystal structure of grape dih ...
Vitis vinifera
J. Mol. Biol.
368
1345-1357
2007
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1
1
1
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4
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1
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5
1
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1
2
1
1
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1
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5
1
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667201
Takahashi
Evaluation of metabolic altera ...
Oryza sativa
Ann. Bot.
98
819-825
2006
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2
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670520
Lo Piero
Gene characterization, analysi ...
Citrus sinensis
Phytochemistry
67
684-695
2006
-
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-
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1
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2
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2
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1
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1
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1
2
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1
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1
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669664
Shimada
A comprehensive analysis of si ...
Lotus japonicus
J. Exp. Bot.
56
2573-2585
2005
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1
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