Information on EC 3.6.5.5 - dynamin GTPase and Organism(s) Homo sapiens and UniProt Accession P50570

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Homo sapiens
UNIPROT: P50570


The taxonomic range for the selected organisms is: Homo sapiens

The enzyme appears in selected viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.6.5.5
-
RECOMMENDED NAME
GeneOntology No.
dynamin GTPase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
GTP + H2O = GDP + phosphate
show the reaction diagram
analysis of the GTPase mechanism of MxA
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphoric ester
SYSTEMATIC NAME
IUBMB Comments
GTP phosphohydrolase (vesicle-releasing)
An enzyme with a molecular mass of about 100 kDa that is involved in endocytosis and is instrumental in pinching off membrane vesicles.
CAS REGISTRY NUMBER
COMMENTARY hide
9059-32-9
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
-
centronuclear myopathy is a genetically heterogeneous disorder associated with general skeletal muscle weakness, type I fiber predominance and atrophy, and abnormally centralized nuclei. Autosomal dominant centronuclear myopathy is due to mutations in the large GTPase dynamin 2. In addition to centronuclear myopathy, dissimilar DNM2 mutations are associated with Charcot-Marie-Tooth peripheral neuropathy (CMTD1B and CMT2M), suggesting a tissue-specific impact of the mutations, possible clinical overlap, phenotypes, overview
physiological function
-
the large GTPase dynamin 2 is a mechanochemical enzyme regulating cytoskeleton and membrane trafficking in cells. The enzyme acts as a mechanochemical enzyme involved in membrane fission, and is therefore a key player for endosome formation and membrane trafficking from the plasma membrane and the trans-Golgi network
evolution
malfunction
metabolism
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
GTP + H2O
GDP + phosphate
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
GTP + H2O
GDP + phosphate
show the reaction diagram
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(5Z)-3-(prop-2-en-1-yl)-5-(quinoxalin-2-ylmethylidene)-2-thioxo-1,3-thiazolidin-4-one
-
-
(5Z)-3-benzyl-5-(2H-chromen-3-ylmethylidene)-2-thioxo-1,3-thiazolidin-4-one
-
-
(5Z)-3-benzyl-5-(3,5-dichlorobenzylidene)-2-thioxo-1,3-thiazolidin-4-one
-
-
(5Z)-3-benzyl-5-(4-bromobenzylidene)-2-thioxo-1,3-thiazolidin-4-one
-
-
(5Z)-3-benzyl-5-(biphenyl-4-ylmethylidene)-2-thioxo-1,3-thiazolidin-4-one
-
-
(5Z)-3-benzyl-5-(quinoxalin-2-ylmethylidene)-2-thioxo-1,3-thiazolidin-4-one
-
-
(5Z)-3-benzyl-5-[(5-chloro-1-benzothiophen-3-yl)methylidene]-2-thioxo-1,3-thiazolidin-4-one
-
-
(5Z)-3-benzyl-5-[(6,8-dichloro-4-oxo-4H-chromen-3-yl)methylidene]-2-thioxo-1,3-thiazolidin-4-one
-
-
(5Z)-3-benzyl-5-[4-[3-(dimethylamino)propoxy]benzylidene]-2-thioxo-1,3-thiazolidin-4-one
-
-
(5Z)-3-ethyl-5-(quinoxalin-2-ylmethylidene)-2-thioxo-1,3-thiazolidin-4-one
-
-
(5Z)-5-(2,1,3-benzothiadiazol-5-ylmethylidene)-2-thioxo-1,3-thiazolidin-4-one
-
-
(5Z)-5-(2,1,3-benzothiadiazol-5-ylmethylidene)-3-(prop-2-en-1-yl)-2-thioxo-1,3-thiazolidin-4-one
-
-
(5Z)-5-(2,1,3-benzothiadiazol-5-ylmethylidene)-3-benzyl-2-thioxo-1,3-thiazolidin-4-one
-
-
(5Z)-5-(2,1,3-benzothiadiazol-5-ylmethylidene)-3-ethyl-2-thioxo-1,3-thiazolidin-4-one
-
-
(5Z)-5-(2,1,3-benzoxadiazol-5-ylmethylidene)-2-thioxo-1,3-thiazolidin-4-one
-
-
(5Z)-5-(2,1,3-benzoxadiazol-5-ylmethylidene)-3-(prop-2-en-1-yl)-2-thioxo-1,3-thiazolidin-4-one
-
-
(5Z)-5-(2,1,3-benzoxadiazol-5-ylmethylidene)-3-benzyl-2-thioxo-1,3-thiazolidin-4-one
-
-
(5Z)-5-(2,1,3-benzoxadiazol-5-ylmethylidene)-3-ethyl-2-thioxo-1,3-thiazolidin-4-one
-
-
(5Z)-5-(2H-chromen-3-ylmethylidene)-2-thioxo-1,3-thiazolidin-4-one
-
-
(5Z)-5-(2H-chromen-3-ylmethylidene)-3-(prop-2-en-1-yl)-2-thioxo-1,3-thiazolidin-4-one
-
-
(5Z)-5-(2H-chromen-3-ylmethylidene)-3-ethyl-2-thioxo-1,3-thiazolidin-4-one
-
-
(5Z)-5-(3,5-dichlorobenzylidene)-2-thioxo-1,3-thiazolidin-4-one
-
-
(5Z)-5-(3,5-dichlorobenzylidene)-3-(prop-2-en-1-yl)-2-thioxo-1,3-thiazolidin-4-one
-
-
(5Z)-5-(3,5-dichlorobenzylidene)-3-ethyl-2-thioxo-1,3-thiazolidin-4-one
-
-
(5Z)-5-(4-bromobenzylidene)-2-thioxo-1,3-thiazolidin-4-one
-
-
(5Z)-5-(4-bromobenzylidene)-3-(prop-2-en-1-yl)-2-thioxo-1,3-thiazolidin-4-one
-
-
(5Z)-5-(4-bromobenzylidene)-3-ethyl-2-thioxo-1,3-thiazolidin-4-one
-
-
(5Z)-5-(biphenyl-4-ylmethylidene)-2-thioxo-1,3-thiazolidin-4-one
-
-
(5Z)-5-(biphenyl-4-ylmethylidene)-3-(prop-2-en-1-yl)-2-thioxo-1,3-thiazolidin-4-one
-
-
(5Z)-5-(biphenyl-4-ylmethylidene)-3-ethyl-2-thioxo-1,3-thiazolidin-4-one
-
-
(5Z)-5-(quinoxalin-2-ylmethylidene)-2-thioxo-1,3-thiazolidin-4-one
-
-
(5Z)-5-[(5-chloro-1-benzothiophen-3-yl)methylidene]-2-thioxo-1,3-thiazolidin-4-one
-
-
(5Z)-5-[(5-chloro-1-benzothiophen-3-yl)methylidene]-3-(prop-2-en-1-yl)-2-thioxo-1,3-thiazolidin-4-one
-
-
(5Z)-5-[(5-chloro-1-benzothiophen-3-yl)methylidene]-3-ethyl-2-thioxo-1,3-thiazolidin-4-one
-
-
(5Z)-5-[(6,8-dichloro-4-oxo-4H-chromen-3-yl)methylidene]-2-thioxo-1,3-thiazolidin-4-one
-
-
(5Z)-5-[(6,8-dichloro-4-oxo-4H-chromen-3-yl)methylidene]-3-(prop-2-en-1-yl)-2-thioxo-1,3-thiazolidin-4-one
-
-
(5Z)-5-[(6,8-dichloro-4-oxo-4H-chromen-3-yl)methylidene]-3-ethyl-2-thioxo-1,3-thiazolidin-4-one
-
-
(5Z)-5-[4-[3-(dimethylamino)propoxy]benzylidene]-2-thioxo-1,3-thiazolidin-4-one
-
-
(5Z)-5-[4-[3-(dimethylamino)propoxy]benzylidene]-3-(prop-2-en-1-yl)-2-thioxo-1,3-thiazolidin-4-one
-
-
(5Z)-5-[4-[3-(dimethylamino)propoxy]benzylidene]-3-ethyl-2-thioxo-1,3-thiazolidin-4-one
-
-
3-sulfo-N-(2-hydroxyethyl)-1,8-naphthalimide
-
-
3-[(5Z)-4-oxo-5-(quinoxalin-2-ylmethylidene)-2-thioxo-1,3-thiazolidin-3-yl]propanoic acid
-
-
3-[(5Z)-5-(2,1,3-benzothiadiazol-5-ylmethylidene)-4-oxo-2-thioxo-1,3-thiazolidin-3-yl]propanoic acid
-
-
3-[(5Z)-5-(2,1,3-benzoxadiazol-5-ylmethylidene)-4-oxo-2-thioxo-1,3-thiazolidin-3-yl]propanoic acid
-
-
3-[(5Z)-5-(2H-chromen-3-ylmethylidene)-4-oxo-2-thioxo-1,3-thiazolidin-3-yl]propanoic acid
-
-
3-[(5Z)-5-(3,5-dichlorobenzylidene)-4-oxo-2-thioxo-1,3-thiazolidin-3-yl]propanoic acid
-
-
3-[(5Z)-5-(4-bromobenzylidene)-4-oxo-2-thioxo-1,3-thiazolidin-3-yl]propanoic acid
-
-
3-[(5Z)-5-(biphenyl-4-ylmethylidene)-4-oxo-2-thioxo-1,3-thiazolidin-3-yl]propanoic acid
-
-
3-[(5Z)-5-[(5-chloro-1-benzothiophen-3-yl)methylidene]-4-oxo-2-thioxo-1,3-thiazolidin-3-yl]propanoic acid
-
-
3-[(5Z)-5-[(6,8-dichloro-4-oxo-4H-chromen-3-yl)methylidene]-4-oxo-2-thioxo-1,3-thiazolidin-3-yl]propanoic acid
-
-
3-[(5Z)-5-[4-[3-(dimethylamino)propoxy]benzylidene]-4-oxo-2-thioxo-1,3-thiazolidin-3-yl]propanoic acid
-
-
4-amino-3-sulfo-N-(2-(dimethylamino)ethyl)-1,8-naphthalimide
-
-
4-amino-3-sulfo-N-(2-(piperazin-1-yl)ethyl)-1,8-naphthalimide
-
-
4-amino-3-sulfo-N-(2-aminobenzyl)-1,8-naphthalimide
-
-
4-amino-3-sulfo-N-(2-aminoethyl)-1,8-naphthalimide
-
-
4-amino-3-sulfo-N-(2-carboxyethyl)-1,8-naphthalimide
-
-
4-amino-3-sulfo-N-(2-hydroxyethyl)-1,8-naphthalimide
-
-
4-amino-3-sulfo-N-(2-mercaptoethyl)-1,8-naphthalimide
-
-
4-amino-3-sulfo-N-(2-methoxyethyl)-1,8-naphthalimide
-
-
4-amino-3-sulfo-N-(3-aminobenzyl)-1,8-naphthalimide
-
-
4-amino-3-sulfo-N-(3-hydroxyphenyl)-1,8-naphthalimide
-
-
4-amino-3-sulfo-N-(3-hydroxypropyl)-1,8-naphthalimide
-
-
4-amino-3-sulfo-N-(4-aminobenzyl)-1,8-naphthalimide
-
-
4-amino-3-sulfo-N-(4-carboxybenzyl)-1,8-naphthalimide
-
-
4-amino-3-sulfo-N-(4-hydroxybenzyl)-1,8-naphthalimide
-
-
4-amino-3-sulfo-N-(4-hydroxyphenyl)-1,8-naphthalimide
-
-
4-amino-3-sulfo-N-(4-methoxyphenyl)-1,8-naphthalimide
-
-
4-amino-3-sulfo-N-(5-hydroxyhexyl)-1,8-naphthalimide
-
-
4-amino-3-sulfo-N-(5-hydroxypentyl)-1,8-naphthalimide
-
-
4-amino-3-sulfo-N-(carboxymethyl)-1,8-naphthalimide
-
-
4-amino-3-sulfo-N-(phenethyl)-1,8-naphthalimide
-
-
4-amino-3-sulfo-N-benzyl-1,8-naphthalimide
-
-
4-amino-3-sulfo-N-phenyl-1,8-naphthalimide
-
-
4-sulfo-N-(2-hydroxyethyl)-1,8-naphthalimide
-
-
dynasore
heterotrimeric G protein betagamma subunit complex
-
oligomerization of dynamin prevents inhibition
-
N-(2-hydroxyethyl)-1,8-naphthalimide
-
-
oligomeric beta-amyloid
-
induces dynamin 1 cleavage and depletion, 0.001 mM of A-705253 potently inhibits oligomeric beta-amyloid-induced dynamin 1 cleavage
-
[(5Z)-4-oxo-5-(quinoxalin-2-ylmethylidene)-2-thioxo-1,3-thiazolidin-3-yl]acetic acid
-
-
[(5Z)-5-(2,1,3-benzothiadiazol-5-ylmethylidene)-4-oxo-2-thioxo-1,3-thiazolidin-3-yl]acetic acid
-
-
[(5Z)-5-(2,1,3-benzoxadiazol-5-ylmethylidene)-4-oxo-2-thioxo-1,3-thiazolidin-3-yl]acetic acid
-
-
[(5Z)-5-(2H-chromen-3-ylmethylidene)-4-oxo-2-thioxo-1,3-thiazolidin-3-yl]acetic acid
-
-
[(5Z)-5-(3,5-dichlorobenzylidene)-4-oxo-2-thioxo-1,3-thiazolidin-3-yl]acetic acid
-
-
[(5Z)-5-(4-bromobenzylidene)-4-oxo-2-thioxo-1,3-thiazolidin-3-yl]acetic acid
-
-
[(5Z)-5-(biphenyl-4-ylmethylidene)-4-oxo-2-thioxo-1,3-thiazolidin-3-yl]acetic acid
-
-
[(5Z)-5-[(5-chloro-1-benzothiophen-3-yl)methylidene]-4-oxo-2-thioxo-1,3-thiazolidin-3-yl]acetic acid
-
-
[(5Z)-5-[(6,8-dichloro-4-oxo-4H-chromen-3-yl)methylidene]-4-oxo-2-thioxo-1,3-thiazolidin-3-yl]acetic acid
-
-
[(5Z)-5-[4-[3-(dimethylamino)propoxy]benzylidene]-4-oxo-2-thioxo-1,3-thiazolidin-3-yl]acetic acid
-
-
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
antibodies
-
stimulate by crosslinking through the C-terminal proline-rich domain of dynamin
-
microtubule
-
activates GTPase activity
-
phosphatidylinositol-4,5-bisphosphate
-
activates
Phospholipid
-
mixed-lineage kinase 2-SH2 domain binds dynamin and greatly enhances activation of GTPase by phospholipid
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0034 - 2.115
GTP
additional information
additional information
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0003 - 3.08
GTP
additional information
additional information
-
kinetic data, temperature-dependent effects on kinetic parameters
-
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.05
(5Z)-3-benzyl-5-(3,5-dichlorobenzylidene)-2-thioxo-1,3-thiazolidin-4-one
Homo sapiens;
-
pH and temperature not specified in the publication
0.134
(5Z)-3-benzyl-5-(4-bromobenzylidene)-2-thioxo-1,3-thiazolidin-4-one
Homo sapiens;
-
pH and temperature not specified in the publication
0.0074
(5Z)-3-benzyl-5-[(6,8-dichloro-4-oxo-4H-chromen-3-yl)methylidene]-2-thioxo-1,3-thiazolidin-4-one
Homo sapiens;
-
pH and temperature not specified in the publication
0.022
(5Z)-3-benzyl-5-[4-[3-(dimethylamino)propoxy]benzylidene]-2-thioxo-1,3-thiazolidin-4-one
Homo sapiens;
-
pH and temperature not specified in the publication
0.0074
(5Z)-5-(2,1,3-benzoxadiazol-5-ylmethylidene)-2-thioxo-1,3-thiazolidin-4-one
Homo sapiens;
-
pH and temperature not specified in the publication
0.053
(5Z)-5-(2,1,3-benzoxadiazol-5-ylmethylidene)-3-benzyl-2-thioxo-1,3-thiazolidin-4-one
Homo sapiens;
-
pH and temperature not specified in the publication
0.09
(5Z)-5-(2H-chromen-3-ylmethylidene)-2-thioxo-1,3-thiazolidin-4-one
Homo sapiens;
-
pH and temperature not specified in the publication
0.0075
(5Z)-5-(3,5-dichlorobenzylidene)-2-thioxo-1,3-thiazolidin-4-one
Homo sapiens;
-
pH and temperature not specified in the publication
0.031
(5Z)-5-(3,5-dichlorobenzylidene)-3-(prop-2-en-1-yl)-2-thioxo-1,3-thiazolidin-4-one
Homo sapiens;
-
pH and temperature not specified in the publication
0.0031
(5Z)-5-(3,5-dichlorobenzylidene)-3-ethyl-2-thioxo-1,3-thiazolidin-4-one
Homo sapiens;
-
pH and temperature not specified in the publication
0.043
(5Z)-5-(quinoxalin-2-ylmethylidene)-2-thioxo-1,3-thiazolidin-4-one
Homo sapiens;
-
pH and temperature not specified in the publication
0.0055
(5Z)-5-[(5-chloro-1-benzothiophen-3-yl)methylidene]-2-thioxo-1,3-thiazolidin-4-one
Homo sapiens;
-
pH and temperature not specified in the publication
0.029
(5Z)-5-[(6,8-dichloro-4-oxo-4H-chromen-3-yl)methylidene]-2-thioxo-1,3-thiazolidin-4-one
Homo sapiens;
-
pH and temperature not specified in the publication
0.0051
(5Z)-5-[(6,8-dichloro-4-oxo-4H-chromen-3-yl)methylidene]-3-ethyl-2-thioxo-1,3-thiazolidin-4-one
Homo sapiens;
-
pH and temperature not specified in the publication
0.0045
(5Z)-5-[4-[3-(dimethylamino)propoxy]benzylidene]-3-(prop-2-en-1-yl)-2-thioxo-1,3-thiazolidin-4-one
Homo sapiens;
-
pH and temperature not specified in the publication
0.0071
(5Z)-5-[4-[3-(dimethylamino)propoxy]benzylidene]-3-ethyl-2-thioxo-1,3-thiazolidin-4-one
Homo sapiens;
-
pH and temperature not specified in the publication
0.3
3-sulfo-N-(2-hydroxyethyl)-1,8-naphthalimide
Homo sapiens;
-
pH 7.4, 37°C
0.0235
3-[(5Z)-5-(2H-chromen-3-ylmethylidene)-4-oxo-2-thioxo-1,3-thiazolidin-3-yl]propanoic acid
Homo sapiens;
-
pH and temperature not specified in the publication
0.0044
3-[(5Z)-5-(3,5-dichlorobenzylidene)-4-oxo-2-thioxo-1,3-thiazolidin-3-yl]propanoic acid
Homo sapiens;
-
pH and temperature not specified in the publication
0.096
3-[(5Z)-5-(4-bromobenzylidene)-4-oxo-2-thioxo-1,3-thiazolidin-3-yl]propanoic acid
Homo sapiens;
-
pH and temperature not specified in the publication
0.042
3-[(5Z)-5-(biphenyl-4-ylmethylidene)-4-oxo-2-thioxo-1,3-thiazolidin-3-yl]propanoic acid
Homo sapiens;
-
pH and temperature not specified in the publication
0.048
3-[(5Z)-5-[(5-chloro-1-benzothiophen-3-yl)methylidene]-4-oxo-2-thioxo-1,3-thiazolidin-3-yl]propanoic acid
Homo sapiens;
-
pH and temperature not specified in the publication
0.01
3-[(5Z)-5-[(6,8-dichloro-4-oxo-4H-chromen-3-yl)methylidene]-4-oxo-2-thioxo-1,3-thiazolidin-3-yl]propanoic acid
Homo sapiens;
-
pH and temperature not specified in the publication
0.509
4-amino-3-sulfo-N-(2-(dimethylamino)ethyl)-1,8-naphthalimide
Homo sapiens;
-
pH 7.4, 37°C
0.155
4-amino-3-sulfo-N-(2-aminobenzyl)-1,8-naphthalimide
Homo sapiens;
-
pH 7.4, 37°C
0.3
4-amino-3-sulfo-N-(2-aminoethyl)-1,8-naphthalimide
Homo sapiens;
-
pH 7.4, 37°C
0.094
4-amino-3-sulfo-N-(2-carboxyethyl)-1,8-naphthalimide
Homo sapiens;
-
pH 7.4, 37°C
0.193
4-amino-3-sulfo-N-(2-hydroxyethyl)-1,8-naphthalimide
Homo sapiens;
-
pH 7.4, 37°C
0.088
4-amino-3-sulfo-N-(3-aminobenzyl)-1,8-naphthalimide
Homo sapiens;
-
pH 7.4, 37°C
0.159
4-amino-3-sulfo-N-(3-hydroxyphenyl)-1,8-naphthalimide
Homo sapiens;
-
pH 7.4, 37°C
0.093
4-amino-3-sulfo-N-(3-hydroxypropyl)-1,8-naphthalimide
Homo sapiens;
-
pH 7.4, 37°C
0.123
4-amino-3-sulfo-N-(4-aminobenzyl)-1,8-naphthalimide
Homo sapiens;
-
pH 7.4, 37°C
0.0185
4-amino-3-sulfo-N-(4-carboxybenzyl)-1,8-naphthalimide
Homo sapiens;
-
pH 7.4, 37°C
0.0448
4-amino-3-sulfo-N-(4-hydroxybenzyl)-1,8-naphthalimide
Homo sapiens;
-
pH 7.4, 37°C
0.296
4-amino-3-sulfo-N-(4-hydroxyphenyl)-1,8-naphthalimide
Homo sapiens;
-
pH 7.4, 37°C
0.113
4-amino-3-sulfo-N-(5-hydroxyhexyl)-1,8-naphthalimide
Homo sapiens;
-
pH 7.4, 37°C
0.062
4-amino-3-sulfo-N-(5-hydroxypentyl)-1,8-naphthalimide
Homo sapiens;
-
pH 7.4, 37°C
0.0191
4-amino-3-sulfo-N-(carboxymethyl)-1,8-naphthalimide
Homo sapiens;
-
pH 7.4, 37°C
0.0703
4-amino-3-sulfo-N-benzyl-1,8-naphthalimide
Homo sapiens;
-
pH 7.4, 37°C
0.3
4-amino-3-sulfo-N-phenyl-1,8-naphthalimide
Homo sapiens;
-
pH 7.4, 37°C
0.3
4-sulfo-N-(2-hydroxyethyl)-1,8-naphthalimide
Homo sapiens;
-
pH 7.4, 37°C
0.0055
[(5Z)-5-(3,5-dichlorobenzylidene)-4-oxo-2-thioxo-1,3-thiazolidin-3-yl]acetic acid
Homo sapiens;
-
pH and temperature not specified in the publication
0.073
[(5Z)-5-(biphenyl-4-ylmethylidene)-4-oxo-2-thioxo-1,3-thiazolidin-3-yl]acetic acid
Homo sapiens;
-
pH and temperature not specified in the publication
0.0123
[(5Z)-5-[(5-chloro-1-benzothiophen-3-yl)methylidene]-4-oxo-2-thioxo-1,3-thiazolidin-3-yl]acetic acid
Homo sapiens;
-
pH and temperature not specified in the publication
0.0034
[(5Z)-5-[(6,8-dichloro-4-oxo-4H-chromen-3-yl)methylidene]-4-oxo-2-thioxo-1,3-thiazolidin-3-yl]acetic acid
Homo sapiens;
-
pH and temperature not specified in the publication
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 7.4
-
assay at
7 - 8
-
assay at
7
-
assay at
7.2
-
radioactive assay for the GTPase activity
7.4
-
assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
-
assay at
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
22 - 37
-
both the basal and assembly-stimulated rates of GTP hydrolysis for wild-type dynamin are 10fold lower when assayed at 22°C compared with 37°C, effect of these temperatures on the Km
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
isozymes dymain 1, dymanin 2, and dynamin 3
Manually annotated by BRENDA team
-
isozymes dymanin 2 and dynamin 3
Manually annotated by BRENDA team
dynamin 3 participates in the growth and development of megakaryocytes
Manually annotated by BRENDA team
-
isozymes dymanin 2 and dynamin 3
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
dynamin 2 localizes with lytic granules and polarizes to the natural killer cell-target interface where it regulates the final fusion of lytic granules with the plasma membrane
Manually annotated by BRENDA team
the enzyme is expressed in the cytoplasm and is partly associated with membranes of the smooth endoplasmic reticulum
Manually annotated by BRENDA team
-
dynamin-related protein 1, DRP1
Manually annotated by BRENDA team
-
dynamin-related protein 1, DRP1
Manually annotated by BRENDA team
additional information
-
DLP-1 is a membrane-interacting protein
-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40923
-
x * 40923, nucleotide-free GTPase-GTPase effector domain fusion protein of Drp1, mass spectrometry
45000
-
4 * 80000, SDS-PAGE, 4 * 45000, isolated PH-like domain, SDS-PAGE
80000
-
4 * 80000, SDS-PAGE, 4 * 45000, isolated PH-like domain, SDS-PAGE
100000
330000
-
recombinant enzyme, gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
heterotetramer
-
Dyn1 can interfere with Dyn2 or Dyn2 mutants, Dyn2-Dyn1, Dyn2S45N-Dyn1 and Dyn2-Dyn1I690K heteromers are formed
homotetramer
-
-
monomer
-
1 * isolated GTPase domain, SDS-PAGE
tetramer
additional information
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
purified recombinant dynamin-related protein 1 GTPase-GTPase effector domain fusion protein, sitting drop vapour diffusion method, mixing of 0.0004 ml of protein solution and reservoir solution, the latter containing 0.2 M lithium sulfate, 0.1 M Bis-Tris, pH 5.5, 25% v/v PEG 3350 or 0.1 M sodium citrate, pH 5.5, 20% PEG 3000, 20°C, X-ray diffraction structure determination and analysis at 2.67 A resolution
-
purified wild-type and mutants of DNM1L isoform 2, mixing of 0.001 ml of 1.2 mg/ml protein with 0.001 ml of reservoir solution containing 0.1 M sodium citrate pH 5, 27.5% PEG 3000, equilibration against 0.4 ml of reservoir solution, 3-5 days, for co-crystallization of the enzyme with a non-hydrolyzable GTP analogue the protein is incubated with 1 mM GMP-PNP and 4 mM MgCl2, and then purified by gel filtration, X-ray diffraction structure determination and analysis at 2.3 A resolution, molecular replacement using human dynamin-1 structure, PDB ID 3SNH
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant dynamin-1
-
recombinant GST- and His-tagged MxA from Escherichia coli strain BL21(DE3) by nickel affinity and glutathione affinity chromatography, and gel filtration
recombinant His-tagged DLP-1 isozyme 2 from Escherichia coli strain C41 (DE3) by nickel affinity chromatography, anion exchange chromatography, and gel filtration
-
recombinant nucleotide-free GTPase-GTPase effector domain fusion protein of human Drp1, and wild-type and mutant full-length enzymes from Escherichia coli strain BL21(DE3)
-
recombinant wild-type and K44A mutant dynamin-1, expressed in Sf9 cells
-
using Ni-NTA and hydroxyapatite chromatography
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
dynamin 2 genotyping
-
expressed in Vero cells
-
a 6-His N-terminal-tagged human dynamin2, lacking the C-terminal proline-rich domain, is constructed, cloned into the pET28a vector for expression in Escherichia coli BL21DE3 cells; human dynamin is expressed in SF9 insect cells, using the Bac-to-Bac baculovirus expression system a full-length cDNA encoding human dynamin1 containing a 6His-tag is subcloned into pFastBac, a bacmid is generated after transposition in Escherichia coli
-
expressed in a fibroblastoid cell line and in embryonic stem cells
-
expressed in CHO cells
-
expressed in umbilical cord blood CD34+ cells
expression in COS-7 cells
-
expression of His-tagged DLP-1 isozyme 2 in Escherichia coli strain C41 (DE3)
-
expression of wild-type and K44A mutant dynamin in HeLa cells
-
expression of wild-type and K44A mutant dynamin-1 in HeLa cells and in Sf9 cells
-
gene for dynamin1, expression in HEK293-cre4 cells
-
overexpression in Chineses hamster ovary cells
-
overexpression of human neuronal dynamin in Sf9 insect cells
-
recombinant expression of C-terminally His6-tagged wild-type and mutants of DNM1L isoform 2
-
recombinant expression of GST- and His-tagged MxA in Escherichia coli strain BL21(DE3)
recombinant expression of nucleotide-free GTPase-GTPase effector domain fusion protein of enzyme Drp1, and wild-type and mutant full-length enzymes in Escherichia coli strain BL21(DE3)
-
the vectors pcDNA3.1 and pADT3T7tet are used, recombinant adenoviruses are produced in HEK293-Cre cells
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
K44A
-
dominant negative mutant
D146A
-
site-directed mutagenesis
D190A
-
site-directed mutagenesis
D218A
-
site-directed mutagenesis
D250N
site-directed mutagenesis, a monomeric mutant, loss of nucleotide binding
D253N
site-directed mutagenesis, a monomeric mutant
DynII690K
-
point mutation to render Dyn1 defective in oligomerization and self-assembly
E81A
-
site-directed mutagenesis
E81A/E82A
-
site-directed mutagenesis
G149A
-
site-directed mutagenesis
G255E
site-directed mutagenesis, a monomeric mutant
G350D
-
middle domain mutant, the mutant shows impaired GTPase activity, but unaffected protein tetramerization and liposome-binding properties
I697A
-
mutation inhibits oligomerization substantially
K216A
-
site-directed mutagenesis
K83A
site-directed mutagenesis, the mutant shows reduced dimerization
M527D
site-directed mutagenesis, a monomeric mutant
M527D/D250N
site-directed mutagenesis, a monomeric mutant
M527D/D250N/D253N
site-directed mutagenesis, a monomeric mutant
M527D/D253N
site-directed mutagenesis, a monomeric mutant
M527D/K83A
site-directed mutagenesis, a monomeric mutant
M527D/T103A
site-directed mutagenesis, a monomeric mutant
N246A
-
site-directed mutagenesis
Q34A
-
site-directed mutagenesis
R365S
-
middle domain mutant, the mutant shows impaired GTPase activity, but unaffected protein tetramerization and liposome-binding properties
R399A
-
the mutant displays a severe defect in quaternary structure in solution and on membranes, no detectable membrane tubulation is observed with R399A, nor is R399A capable of mediating membrane fission, R399A is also unable to inhibit fission catalyzed by wild type dynamin
S35A
-
site-directed mutagenesis
S39A
-
site-directed mutagenesis
S40A
-
site-directed mutagenesis
S45N
-
the mutant is defective in GTP binding
S61A
-
mutation lowers the rate of assembly-stimulated GTP hydrolysis without altering the Km for GTP, kinetic data
S61D
-
kinetic data
T103A
site-directed mutagenesis, a monomeric mutant
T141A
-
mutation increases the rate of assembly-stimulated GTP hydrolysis without altering the Km for GTP, kinetic data
T141D
-
kinetic data
T59A
-
site-directed mutagenesis
T65A
-
mutation dramatically lowers both the rate of assembly-stimulated GTP hydrolysis and the affinity for GTP, kinetic data
T65D
-
mutation dramatically lowers both the rate of assembly-stimulated GTP hydrolysis and the affinity for GTP, kinetic data
T65H
-
mutation dramatically lowers both the rate of assembly-stimulated GTP hydrolysis and the affinity for GTP, kinetic data
V268M
site-directed mutagenesis, the mutant shows reduced dimerization
additional information