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Literature summary for 3.6.5.5 extracted from

  • Zhang, Y.; Gao, X.; Garavito, R.M.
    Biochemical characterization of human dynamin-like protein 1 (2011), J. Biochem., 150, 627-633.
    View publication on PubMed
Search for more literature for 3.6.5.5

Cloned(Commentary)

Cloned (Comment) Organism
expression of His-tagged DLP-1 isozyme 2 in Escherichia coli strain C41 (DE3) Homo sapiens

Protein Variants

Protein Variants Comment Organism
G350D middle domain mutant, the mutant shows impaired GTPase activity, but unaffected protein tetramerization and liposome-binding properties Homo sapiens
K38A a GTPase null mutant Homo sapiens
additional information deletion of the PH-like domain totally abolishes the GTPase activity of the mutant. The DLP-1 G350D and the R365S mutations do not markedly impact DLP-1 tetramer, nor are the reduced GTPase activities a consequence of the loss of the protein's quaternary structure Homo sapiens
R365S middle domain mutant, the mutant shows impaired GTPase activity, but unaffected protein tetramerization and liposome-binding properties Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
additional information DLP-1 is a membrane-interacting protein Homo sapiens
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 4 * 80000, SDS-PAGE, 4 * 45000, isolated PH-like domain, SDS-PAGE Homo sapiens
80000
-
 4 * 80000, SDS-PAGE, 4 * 45000, isolated PH-like domain, SDS-PAGE Homo sapiens
330000
-
 recombinant enzyme, gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GTP + H2O Homo sapiens
-
 GDP + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged DLP-1 isozyme 2 from Escherichia coli strain C41 (DE3) by nickel affinity chromatography, anion exchange chromatography, and gel filtration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + H2O
-
 Homo sapiens GDP + phosphate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * isolated GTPase domain, SDS-PAGE Homo sapiens
More DLP-1 contains a GTPase domain, a middle domain, a putative PH-like domain, and a GTPase effector domain, GED Homo sapiens
tetramer 4 * 80000, SDS-PAGE, 4 * 45000, isolated PH-like domain, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
DLP-1
-
 Homo sapiens
dynamin-like protein 1
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 assay at room temperature Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8 assay at Homo sapiens

General Information

General Information Comment Organism
malfunction middle domain mutants G350D, R365S and PH, lacking the putative PH-like domain, severely impair the GTPase activity, but have no obvious effects on protein tetramerization and liposome-binding properties, these mutants probably affect protein intra-molecular interactions Homo sapiens
additional information proper domain-domain interactions are important for DLP-1 GTPase activity Homo sapiens
physiological function dynamin-like protein 1, DLP-1, is involved in the fission of mitochondrial outer membranes, a process that helps to maintain mitochondrial morphology and to reduce the accumulation of functional and structural defects in mitochondria Homo sapiens