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Literature summary for 3.6.5.5 extracted from

  • Koirala, S.; Guo, Q.; Kalia, R.; Bui, H.T.; Eckert, D.M.; Frost, A.; Shaw, J.M.
    Interchangeable adaptors regulate mitochondrial dynamin assembly for membrane scission (2013), Proc. Natl. Acad. Sci. USA, 110, E1342-E1351 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.6.5.5

Activating Compound

Activating Compound Comment Organism Structure
additional information interchangeable adaptors regulate mitochondrial dynamin assembly for membrane scission Homo sapiens
additional information interchangeable adaptors regulate mitochondrial dynamin assembly for membrane scission Saccharomyces cerevisiae

Cloned(Commentary)

Cloned (Comment) Organism
gene DNM1 Saccharomyces cerevisiae
gene DNM1, recombinant expression of Drp1 containing an N-terminal PreScission protease cleavage site and a FLAG-One-Strep-tag (IBA) from plasmid pRS415-DNM1 in Saccharomyces cerevisiae strain JSY9612 lacking all fission proteins (MATa, can1, ade2, trp1, ura3, his3, leu2, pep4::HIS3, prb1::LEU2, bar1::HISG, lys2::GAL1/10-GAL4), coexpression with Saccharomyces cerevisiae mitochondrial fission 1 protein, Fis1 (UniProt ID P40515) Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information recombinant expression of human enzyme Drp1 from plasmid pRS415-DNM1 in Saccharomyces cerevisiae strain JSY9612 lacking all fission proteins (MATa, can1, ade2, trp1, ura3, his3, leu2, pep4::HIS3, prb1::LEU2, bar1::HISG, lys2::GAL1/10-GAL4), coexpression with Saccharomyces cerevisiae mitochondrial fission 1 protein, Fis1 (UniProt ID P40515). Identification of the minimal combination of DRPs and adaptors sufficient for mitochondrial fission. Yeast Fis1 is dispensable for mitochondrial membrane scission. Mdv1, Mff, and MiDs paired individually with their respective DRPs are interchangeable, in that each is sufficient to catalyze fission. Importantly, coassembly of an MiD protein with Drp1 dramatically decreases the diameter of the Drp1 structures formed Saccharomyces cerevisiae
additional information the enzyme is cloned with Tom20 used as an N-terminal mitochondrial outer membrane anchor, or amino acids 131-155 of yeast Fis1 used as a C-terminal mitochondrial outer membrane anchor Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol Drp1 is a cytosolic protein that translocates to the outer mitochondrial membrane Homo sapiens 5829
-
mitochondrial outer membrane Drp1 is a cytosolic protein that translocates to the outer mitochondrial membrane Homo sapiens 5741
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Homo sapiens
Mg2+ required Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
169624
-
 recombinant enzyme, analytical equilibrium sedimentation analysis Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GTP + H2O Homo sapiens
-
 GDP + phosphate
-
 ?
GTP + H2O Saccharomyces cerevisiae
-
 GDP + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens O00429
-
-
Saccharomyces cerevisiae P54861
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant N-terminally Strep-tagged Drp1 from Saccharomyces cerevisiae strain JSY9612 by affinity chromatography, followed by tag cleavage by PreScission protease, and dialysis Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + H2O
-
 Homo sapiens GDP + phosphate
-
 ?
GTP + H2O
-
 Saccharomyces cerevisiae GDP + phosphate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 78000-85000, recombinant enzyme, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
dynamin-related protein 1 isoform 3
-
 Homo sapiens
dynamin-related protein 1 isoform 3
-
 Saccharomyces cerevisiae
fission dynamin
-
 Homo sapiens
GTPase
-
 Homo sapiens
GTPase
-
 Saccharomyces cerevisiae
hDrp1 isoform 3
-
 Homo sapiens
hDrp1 isoform 3
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens
37
-
 assay at Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Homo sapiens
7.4
-
 assay at Saccharomyces cerevisiae

General Information

General Information Comment Organism
physiological function mitochondrial fission is mediated by the dynamin-related GTPases Dnm1/Drp1 (yeast/mammals), which form spirals around constricted sites on mitochondria. Additional membrane-associated adaptor proteins (Fis1, Mdv1, Mff, and MiDs) are required to recruit these GTPases from the cytoplasm to the mitochondrial surface. Adaptors regulate mitochondrial dynamin assembly for membrane scission. An adaptor protein alters the architecture of a mitochondrial dynamin GTPase polymer in a manner that can facilitate membrane constriction and severing activity. Interchangeable adaptors regulate mitochondrial dynamin assembly for membrane scission Homo sapiens
physiological function mitochondrial fission is mediated by the dynamin-related GTPases Dnm1/Drp1 (yeast/mammals), which form spirals around constricted sites on mitochondria. Additional membrane-associated adaptor proteins (Fis1, Mdv1, Mff, and MiDs) are required to recruit these GTPases from the cytoplasm to the mitochondrial surface. Adaptors regulate mitochondrial dynamin assembly for membrane scission. An adaptor protein alters the architecture of a mitochondrial dynamin GTPase polymer in a manner that can facilitate membrane constriction and severing activity. Interchangeable adaptors regulate mitochondrial dynamin assembly for membrane scission Saccharomyces cerevisiae