Any feedback?
No. of entries
Information on EC 2.7.9.2 - pyruvate, water dikinase
for references in articles please use BRENDA:EC2.7.9.2
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree 2 Transferases
2.7 Transferring phosphorus-containing groups
2.7.9 Phosphotransferases with paired acceptors
2.7.9.2 pyruvate, water dikinase
IUBMB Comments
A manganese protein.
The enzyme appears in viruses and cellular organisms
- 2.7.9.2
-
gluconeogenesis
-
carboxykinase
-
anaplerotic
- dahp
- pck
-
staphylothermus
- drug development
showSynonyms
phosphoenolpyruvate synthase, phosphoenolpyruvate synthetase, pep synthase, pep synthetase, phosphoenol pyruvate synthetase, water dikinase pyruvate, more
topPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
kinase, pyruvate-water di- (phosphorylating)
-
-
-
-
PEP synthase
PEP synthase
-
-
-
-
PEP synthetase
PEPS
phosphoenolpyruvate synthase
phosphoenolpyruvate synthetase
phosphoenolpyruvic synthase
-
-
-
-
phosphopyruvate synthetase, phosphopyruvate
-
-
-
-
Pps
PpsA
pyruvate, water dikinase
-
-
-
-
pyruvate,water dikinase
-
-
-
-
SSO2792
synthetase, phosphopyruvate
-
-
-
-
TK1292
water dikinase pyruvate
-
-
-
-
PEP synthetase
-
-
-
-
phosphoenolpyruvate synthase
-
-
-
-
phosphoenolpyruvate synthetase
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ATP + pyruvate + H2O = AMP + phosphoenolpyruvate + phosphate
reaction sequence
-
ATP + pyruvate + H2O = AMP + phosphoenolpyruvate + phosphate
identification of phosphohistidine in phosphoenzyme intermediate
-
ATP + pyruvate + H2O = AMP + phosphoenolpyruvate + phosphate
phosphoenolpyruvate synthase catalyzes the conversion of pyruvate to phosphoenolpyruvate (PEP) using a two-step mechanism invoking a phosphorylated-His intermediate
ATP + pyruvate + H2O = AMP + phosphoenolpyruvate + phosphate
mechanism
Escherichia coli B / ATCC 11303
-
-
ATP + pyruvate + H2O = AMP + phosphoenolpyruvate + phosphate
reaction sequence
Escherichia coli B / ATCC 11303
-
-
ATP + pyruvate + H2O = AMP + phosphoenolpyruvate + phosphate
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
phospho group transfer
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
PATHWAY SOURCE
PATHWAYS
BRENDA
KEGG
MetaCyc
gluconeogenesis I, gluconeogenesis II (Methanobacterium thermoautotrophicum), glycolysis I (from glucose 6-phosphate), glycolysis II (from fructose 6-phosphate), glycolysis V (Pyrococcus)
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYSTEMATIC NAME
IUBMB Comments
ATP:pyruvate,water phosphotransferase
A manganese protein.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
CAS REGISTRY NUMBER
COMMENTARY
9013-09-6
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ATP + 2-ketobutyrate + H2O
?
-
Substrates: 8% of the activity with pyruvate
Products: -
Products: -
?
ATP + 2-oxoglutarate + H2O
?
-
Substrates: 2% of the activity with pyruvate
Products: -
Products: -
?
AMP + phosphoenolpyruvate + phosphate
ATP + pyruvate + H2O
-
Substrates: -
Products: -
Products: -
?
AMP + phosphoenolpyruvate + phosphate
ATP + pyruvate + H2O
-
Substrates: the enzyme is involved in the production of pyruvate, phosphoenolpyruvate and ATP from glycerol in Pseudomonas fluorescens
Products: -
Products: -
?
AMP + phosphoenolpyruvate + phosphate
ATP + pyruvate + H2O
-
Substrates: -
Products: -
Products: -
?
AMP + phosphoenolpyruvate + phosphate
ATP + pyruvate + H2O
-
Substrates: the enzyme is involved in the production of pyruvate, phosphoenolpyruvate and ATP from glycerol in Pseudomonas fluorescens
Products: -
Products: -
?
AMP + phosphoenolpyruvate + phosphate
ATP + pyruvate + H2O
-
Substrates: -
Products: -
Products: -
?
AMP + phosphoenolpyruvate + phosphate
ATP + pyruvate + H2O
Substrates: -
Products: -
Products: -
?
AMP + phosphoenolpyruvate + phosphate
ATP + pyruvate + H2O
-
Substrates: CMP, GMP, IMP, UMP, CDP, GDP, IDP, and UDP are inert as the acceptor. Diphosphate can not be substituted for phosphate. The enzyme does not catalyze the diphosphate-dependent formation of pyruvate from phosphoenolpyruvate and AMP
Products: -
Products: -
r
ATP + 3-fluoropyruvate + H2O
AMP + (Z)-fluorophosphoenolpyruvate + phosphate
Substrates: stereochemically specific reaction
Products: -
Products: -
r
ATP + 3-fluoropyruvate + H2O
AMP + (Z)-fluorophosphoenolpyruvate + phosphate
Substrates: 10times slower reaction rate than with pyruvate
Products: -
Products: -
r
ATP + pyruvate + H2O
AMP + phosphoenolpyruvate + phosphate
-
Substrates: -
Products: -
Products: -
?
ATP + pyruvate + H2O
AMP + phosphoenolpyruvate + phosphate
-
Substrates: -
Products: -
Products: -
r
ATP + pyruvate + H2O
AMP + phosphoenolpyruvate + phosphate
Substrates: -
Products: -
Products: -
ir
ATP + pyruvate + H2O
AMP + phosphoenolpyruvate + phosphate
-
Substrates: -
Products: -
Products: -
ir
ATP + pyruvate + H2O
AMP + phosphoenolpyruvate + phosphate
Substrates: -
Products: -
Products: -
r
ATP + pyruvate + H2O
AMP + phosphoenolpyruvate + phosphate
-
Substrates: highly specific
Products: -
Products: -
r
ATP + pyruvate + H2O
AMP + phosphoenolpyruvate + phosphate
-
Substrates: phosphorylated enzyme as an intermediate
Products: -
Products: -
r
ATP + pyruvate + H2O
AMP + phosphoenolpyruvate + phosphate
-
Substrates: equilibrium lies far to the side of phosphoenolpyruvate formation
Products: -
Products: -
r
ATP + pyruvate + H2O
AMP + phosphoenolpyruvate + phosphate
-
Substrates: in the reverse reaction dAMP yields 1% of the rate obtained with AMP, 3'-AMP gives no reaction
Products: -
Products: -
r
ATP + pyruvate + H2O
AMP + phosphoenolpyruvate + phosphate
-
Substrates: enzyme is essential for gluconeogenesis in Escherichia coli and Salmonella typhimurium during the growth on pyruvate, lactate, alanine or serine, in certain circumstances the enzyme may also provide phosphoenolpyruvate under glycolytic conditions
Products: -
Products: -
r
ATP + pyruvate + H2O
AMP + phosphoenolpyruvate + phosphate
Substrates: involved in gluconeogenesis
Products: -
Products: -
r
ATP + pyruvate + H2O
AMP + phosphoenolpyruvate + phosphate
Substrates: essential step in gluconeogenesis if pyruvate or lactate is used as carbon source
Products: -
Products: -
r
ATP + pyruvate + H2O
AMP + phosphoenolpyruvate + phosphate
Substrates: phospho-transfer mechanism, overview
Products: -
Products: -
ir
ATP + pyruvate + H2O
AMP + phosphoenolpyruvate + phosphate
Escherichia coli B / ATCC 11303
-
Substrates: highly specific
Products: -
Products: -
r
ATP + pyruvate + H2O
AMP + phosphoenolpyruvate + phosphate
Escherichia coli B / ATCC 11303
-
Substrates: in the reverse reaction dAMP yields 1% of the rate obtained with AMP, 3'-AMP gives no reaction
Products: -
Products: -
r
ATP + pyruvate + H2O
AMP + phosphoenolpyruvate + phosphate
Escherichia coli B / ATCC 11303
-
Substrates: enzyme is essential for gluconeogenesis in Escherichia coli and Salmonella typhimurium during the growth on pyruvate, lactate, alanine or serine, in certain circumstances the enzyme may also provide phosphoenolpyruvate under glycolytic conditions
Products: -
Products: -
r
ATP + pyruvate + H2O
AMP + phosphoenolpyruvate + phosphate
Escherichia coli B / ATCC 11303
-
Substrates: -
Products: -
Products: -
?
ATP + pyruvate + H2O
AMP + phosphoenolpyruvate + phosphate
Substrates: -
Products: -
Products: -
?
ATP + pyruvate + H2O
AMP + phosphoenolpyruvate + phosphate
-
Substrates: absolutely specific for ATP, highly specific for pyruvate
Products: -
Products: -
r
ATP + pyruvate + H2O
AMP + phosphoenolpyruvate + phosphate
-
Substrates: phosphorylated enzyme as an intermediate
Products: -
Products: -
r
ATP + pyruvate + H2O
AMP + phosphoenolpyruvate + phosphate
-
Substrates: equilibrium lies far to the side of phosphoenolpyruvate formation
Products: -
Products: -
r
ATP + pyruvate + H2O
AMP + phosphoenolpyruvate + phosphate
-
Substrates: involved in gluconeogenesis
Products: -
Products: -
r
ATP + pyruvate + H2O
AMP + phosphoenolpyruvate + phosphate
-
Substrates: -
Products: -
Products: -
r
ATP + pyruvate + H2O
AMP + phosphoenolpyruvate + phosphate
Substrates: -
Products: -
Products: -
?
ATP + pyruvate + H2O
AMP + phosphoenolpyruvate + phosphate
Substrates: -
Products: -
Products: -
?
ATP + pyruvate + H2O
AMP + phosphoenolpyruvate + phosphate
-
Substrates: -
Products: -
Products: -
r
ATP + pyruvate + H2O
AMP + phosphoenolpyruvate + phosphate
-
Substrates: involved in gluconeogenesis
Products: -
Products: -
r
ATP + pyruvate + H2O
AMP + phosphoenolpyruvate + phosphate
-
Substrates: -
Products: -
Products: -
r
ATP + pyruvate + H2O
AMP + phosphoenolpyruvate + phosphate
-
Substrates: -
Products: -
Products: -
r
ATP + pyruvate + H2O
AMP + phosphoenolpyruvate + phosphate
Substrates: -
Products: -
Products: -
ir
ATP + pyruvate + H2O
AMP + phosphoenolpyruvate + phosphate
Substrates: -
Products: -
Products: -
ir
ATP + pyruvate + H2O
AMP + phosphoenolpyruvate + phosphate
-
Substrates: -
Products: -
Products: -
r
additional information
?
-
-
Substrates: no activity with 2-keto-3-methylvalerate, 2-ketoisocaproate, 2-ketomalonate, phenylpyruvate, hydroxyphenylpyruvate, imidazolpyruvate, and indole-3-pyruvate
Products: -
Products: -
?
additional information
?
-
Substrates: neither GTP, CTP, ITP nor UTP is active as phosphoryl donor
Products: -
Products: -
?
additional information
?
-
-
Substrates: neither GTP, CTP, ITP nor UTP is active as phosphoryl donor
Products: -
Products: -
?
additional information
?
-
Substrates: neither GTP, CTP, ITP nor UTP is active as phosphoryl donor
Products: -
Products: -
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
AMP + phosphoenolpyruvate + phosphate
ATP + pyruvate + H2O
-
Substrates: the enzyme is involved in the production of pyruvate, phosphoenolpyruvate and ATP from glycerol in Pseudomonas fluorescens
Products: -
Products: -
?
AMP + phosphoenolpyruvate + phosphate
ATP + pyruvate + H2O
-
Substrates: the enzyme is involved in the production of pyruvate, phosphoenolpyruvate and ATP from glycerol in Pseudomonas fluorescens
Products: -
Products: -
?
ATP + pyruvate + H2O
AMP + phosphoenolpyruvate + phosphate
Substrates: -
Products: -
Products: -
ir
ATP + pyruvate + H2O
AMP + phosphoenolpyruvate + phosphate
-
Substrates: -
Products: -
Products: -
ir
ATP + pyruvate + H2O
AMP + phosphoenolpyruvate + phosphate
-
Substrates: enzyme is essential for gluconeogenesis in Escherichia coli and Salmonella typhimurium during the growth on pyruvate, lactate, alanine or serine, in certain circumstances the enzyme may also provide phosphoenolpyruvate under glycolytic conditions
Products: -
Products: -
r
ATP + pyruvate + H2O
AMP + phosphoenolpyruvate + phosphate
Substrates: involved in gluconeogenesis
Products: -
Products: -
r
ATP + pyruvate + H2O
AMP + phosphoenolpyruvate + phosphate
Substrates: essential step in gluconeogenesis if pyruvate or lactate is used as carbon source
Products: -
Products: -
r
ATP + pyruvate + H2O
AMP + phosphoenolpyruvate + phosphate
Escherichia coli B / ATCC 11303
-
Substrates: enzyme is essential for gluconeogenesis in Escherichia coli and Salmonella typhimurium during the growth on pyruvate, lactate, alanine or serine, in certain circumstances the enzyme may also provide phosphoenolpyruvate under glycolytic conditions
Products: -
Products: -
r
ATP + pyruvate + H2O
AMP + phosphoenolpyruvate + phosphate
-
Substrates: involved in gluconeogenesis
Products: -
Products: -
r
ATP + pyruvate + H2O
AMP + phosphoenolpyruvate + phosphate
-
Substrates: involved in gluconeogenesis
Products: -
Products: -
r
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Co2+
-
requires divalent cations in the forward reaction, 93% of the activity with Mg2+
Mg2+
Mn2+
Ni2+
-
requires divalent cations in the forward reaction, 15% of the activity with Mg2+
additional information
Mg2+
-
divalent metal ion Mg2+ or Mn2+ required for forward reaction
Mg2+
-
requires divalent cations in the forward reaction, Mg2+ is most effective
Mn2+
-
divalent metal ion Mg2+ or Mn2+ required for forward reaction
Mn2+
-
requires divalent cations in the forward reaction, 95% of the activity with Mg2+
additional information
-
enzyme contains sulfhydryl groups essential for activity
additional information
-
Ca2+ and Sr2+ cannot substitute for Mg2+ in the catalytic reaction
additional information
activity of PEPS does not depend on monovalent cations such as K+ and NH4+
additional information
-
activity of PEPS does not depend on monovalent cations such as K+ and NH4+
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Mg2+
-
divalent metal ion Mg2+ or Mn2+ required for forward reaction, inhibition at high concentrations of Mg2+ or Mn2+
Mn2+
-
divalent metal ion Mg2+ or Mn2+ required for forward reaction, inhibition at high concentrations of Mg2+ or Mn2+
Sodium fluoride
inhibition through the formation of a MgF3- complex within the enzyme active site
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
1
ATP
in 100 mM Tris/HCl, pH 7.0, in the presence of 20 mM beta-mercaptoethanol, 6 mM pyruvate, 10 mM ATP and 10 mM MgCl2, at 70°C
0.4
pyruvate
in 100 mM Tris/HCl, pH 7.0, in the presence of 20 mM beta-mercaptoethanol, 6 mM pyruvate, 10 mM ATP and 10 mM MgCl2, at 70°C
additional information
additional information
-
Km-values, both reaction directions, overview
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.683
pyruvate
in 100 mM Tris/HCl, pH 7.0, in the presence of 20 mM beta-mercaptoethanol, 6 mM pyruvate, 10 mM ATP and 10 mM MgCl2, at 70°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.6
2-oxoglutarate
in 100 mM Tris/HCl, pH 7.0, in the presence of 20 mM beta-mercaptoethanol, 6 mM pyruvate, 10 mM ATP and 10 mM MgCl2, at 70°C
2.6
ADP
in 100 mM Tris/HCl, pH 7.0, in the presence of 20 mM beta-mercaptoethanol, 6 mM pyruvate, 10 mM ATP and 10 mM MgCl2, at 70°C
0.5
AMP
in 100 mM Tris/HCl, pH 7.0, in the presence of 20 mM beta-mercaptoethanol, 6 mM pyruvate, 10 mM ATP and 10 mM MgCl2, at 70°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
6.8
7.5
8.4
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
50
90
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
