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Literature summary for 2.7.9.2 extracted from

  • Tjaden, B.; Plagens, A.; Doerr, C.; Siebers, B.; Hensel, R.
    Phosphoenolpyruvate synthetase and pyruvate, phosphate dikinase of Thermoproteus tenax: key pieces in the puzzle of archaeal carbohydrate metabolism (2006), Mol. Microbiol., 60, 287-298.
    View publication on PubMed
Search for more literature for 2.7.9.2

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strains DH5alpha and BL21-CodonPlus(DE3)-RIL Thermoproteus tenax

Inhibitors

Inhibitors Comment Organism Structure
2-oxoglutarate strong inhibition at 1 mM Thermoproteus tenax
ADP strong inhibition at 1 mM Thermoproteus tenax
AMP strong inhibition at 1 mM Thermoproteus tenax

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.4
-
 pyruvate in 100 mM Tris/HCl, pH 7.0, in the presence of 20 mM beta-mercaptoethanol, 6 mM pyruvate, 10 mM ATP and 10 mM MgCl2, at 70°C Thermoproteus tenax
1
-
 ATP in 100 mM Tris/HCl, pH 7.0, in the presence of 20 mM beta-mercaptoethanol, 6 mM pyruvate, 10 mM ATP and 10 mM MgCl2, at 70°C Thermoproteus tenax

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 10 mM, required Thermoproteus tenax
additional information activity of PEPS does not depend on monovalent cations such as K+ and NH4+ Thermoproteus tenax

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
88000
-
 SDS-PAGE, recombinant enzyme, phosphoenzyme intermediate form Thermoproteus tenax
90500
-
 calculated from amino acid sequence Thermoproteus tenax
94000
-
 SDS-PAGE, recombinant enzyme, nonphosphorylated form Thermoproteus tenax
600000
-
 above 600000, gel filtration Thermoproteus tenax

Organism

Organism UniProt Comment Textmining
Thermoproteus tenax Q70WQ8
-
-
Thermoproteus tenax Kra1 Q70WQ8
-
-

Purification (Commentary)

Purification (Comment) Organism
Q-sepharose chromatography Thermoproteus tenax

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + pyruvate + H2O
-
 Thermoproteus tenax AMP + phosphoenolpyruvate + phosphate
-
 ir
ATP + pyruvate + H2O
-
 Thermoproteus tenax Kra1 AMP + phosphoenolpyruvate + phosphate
-
 ir
additional information neither GTP, CTP, ITP nor UTP is active as phosphoryl donor Thermoproteus tenax ?
-
 ?
additional information neither GTP, CTP, ITP nor UTP is active as phosphoryl donor Thermoproteus tenax Kra1 ?
-
 ?

Subunits

Subunits Comment Organism
multimer native enzyme, gel filtration Thermoproteus tenax

Synonyms

Synonyms Comment Organism
PEP synthetase
-
 Thermoproteus tenax
PEPS
-
 Thermoproteus tenax
phosphoenolpyruvate synthetase
-
 Thermoproteus tenax

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.683
-
 pyruvate in 100 mM Tris/HCl, pH 7.0, in the presence of 20 mM beta-mercaptoethanol, 6 mM pyruvate, 10 mM ATP and 10 mM MgCl2, at 70°C Thermoproteus tenax

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Thermoproteus tenax

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.5
-
 AMP in 100 mM Tris/HCl, pH 7.0, in the presence of 20 mM beta-mercaptoethanol, 6 mM pyruvate, 10 mM ATP and 10 mM MgCl2, at 70°C Thermoproteus tenax
0.6
-
 2-oxoglutarate in 100 mM Tris/HCl, pH 7.0, in the presence of 20 mM beta-mercaptoethanol, 6 mM pyruvate, 10 mM ATP and 10 mM MgCl2, at 70°C Thermoproteus tenax
2.6
-
 ADP in 100 mM Tris/HCl, pH 7.0, in the presence of 20 mM beta-mercaptoethanol, 6 mM pyruvate, 10 mM ATP and 10 mM MgCl2, at 70°C Thermoproteus tenax