Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
77000 | - |
2 * 77000, enzyme tends to dissociate to monomers at low protein concentration | Escherichia coli |
150000 | - |
sedimentation equilibrium studies | Escherichia coli |
250000 | - |
gel filtration | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
255fold | Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + pyruvate + H2O = AMP + phosphoenolpyruvate + phosphate | identification of phosphohistidine in phosphoenzyme intermediate | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Escherichia coli |
Storage Stability | Organism |
---|---|
4°C, dephosphorylated form of enzyme, 50 mM Tris/HCl, pH 6.8, 0.2 mM EDTA, 0.2 mM DTT, 1 M sucrose, stable over a period of 12 months | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + pyruvate + H2O | - |
Escherichia coli | AMP + phosphoenolpyruvate + phosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 77000, enzyme tends to dissociate to monomers at low protein concentration | Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
slightly cold-labile | Escherichia coli |
4 | - |
80% remaining activity after 3 days, 67% remaining activity after 1 month, pH 6.8, inactivation is reversible by prolonged incubation at 30°C | Escherichia coli |
30 | - |
stable at | Escherichia coli |