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Literature summary for 2.7.9.2 extracted from

  • Narindrasorasak, S.; Bridger, W.A.
    Phosphoenolypyruvate synthetase of Escherichia coli: molecular weight, subunit composition, and identification of phosphohistidine in phosphoenzyme intermediate (1977), J. Biol. Chem., 252, 3121-3127.
    View publication on PubMed

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
77000
-
2 * 77000, enzyme tends to dissociate to monomers at low protein concentration Escherichia coli
150000
-
sedimentation equilibrium studies Escherichia coli
250000
-
gel filtration Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
255fold Escherichia coli

Reaction

Reaction Comment Organism Reaction ID
ATP + pyruvate + H2O = AMP + phosphoenolpyruvate + phosphate identification of phosphohistidine in phosphoenzyme intermediate Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Escherichia coli

Storage Stability

Storage Stability Organism
4°C, dephosphorylated form of enzyme, 50 mM Tris/HCl, pH 6.8, 0.2 mM EDTA, 0.2 mM DTT, 1 M sucrose, stable over a period of 12 months Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + pyruvate + H2O
-
Escherichia coli AMP + phosphoenolpyruvate + phosphate
-
r

Subunits

Subunits Comment Organism
dimer 2 * 77000, enzyme tends to dissociate to monomers at low protein concentration Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
slightly cold-labile Escherichia coli
4
-
80% remaining activity after 3 days, 67% remaining activity after 1 month, pH 6.8, inactivation is reversible by prolonged incubation at 30°C Escherichia coli
30
-
stable at Escherichia coli