Cloned (Comment) | Organism |
---|---|
gene ppsA, subcloning in Escherichia coli strain JM19, recombinant overexpression in Escherichia coli strain B0013, coexpression with genes aroG and tktA | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
additional information | three critical enzymes of mutated 3-deoxy-D-arabinoheptulosonate-7-phosphate (DAHP) synthase (encoded by aroGfbr), PEP synthase (encoded by ppsA), and transketolase A (encoded by tktA) are modularly overexpressed in Escherichia coli and the resulting recombinant strain produces increased levels of shikimate in an optimized fed-batch fermentation process, overview | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + pyruvate + H2O | Escherichia coli | - |
AMP + phosphoenolpyruvate + phosphate | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P23538 | B0013, gene ppsA | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + pyruvate + H2O | - |
Escherichia coli | AMP + phosphoenolpyruvate + phosphate | - |
ir |
Synonyms | Comment | Organism |
---|---|---|
PEP synthase | - |
Escherichia coli |
phosphoenolpyruvate synthase | - |
Escherichia coli |
PpsA | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Escherichia coli |
General Information | Comment | Organism |
---|---|---|
metabolism | phosphoenolpyruvate produced by the enzyme is required in the shikimate biosynthesis, pathway overview. The expression levels of the enzyme transketolase I (encoded by tktA) and phosphoenolpyruvate synthase (encoded by ppsA) have a critical impact on the phosphoenolpyruvate and erythrose-4-phosphate availability, which are important determinants for in vivo 3-deoxy-D-arabinoheptulosonate-7-phosphate synthase activity | Escherichia coli |