KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
AMP | pH 6.5, 50°C | Pyrococcus horikoshii | |
1 | - |
phosphoenolpyruvate | pH 6.5, 50°C | Pyrococcus horikoshii | |
35 | - |
phosphate | pH 6.5, 50°C | Pyrococcus horikoshii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
670000 | - |
more than 670000 Da | Pyrococcus horikoshii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus horikoshii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pyrococcus horikoshii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.3 | - |
pH 6.5, 50°C, ATP-dependent pyruvate consumption | Pyrococcus horikoshii |
13.3 | - |
pH 6.5, 50°C, AMP-dependent kinase activity | Pyrococcus horikoshii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
AMP + phosphoenolpyruvate + phosphate | CMP, GMP, IMP, UMP, CDP, GDP, IDP, and UDP are inert as the acceptor. Diphosphate can not be substituted for phosphate. The enzyme does not catalyze the diphosphate-dependent formation of pyruvate from phosphoenolpyruvate and AMP | Pyrococcus horikoshii | ATP + pyruvate + H2O | - |
r | |
ATP + pyruvate + H2O | - |
Pyrococcus horikoshii | AMP + phosphoenolpyruvate + phosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 78000, SDS-PAGE | Pyrococcus horikoshii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
assay at | Pyrococcus horikoshii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
the enzyme retains its full activity upon heating at 90°C for 30 min | Pyrococcus horikoshii |
100 | - |
the enzyme retains 92% of full activity upon heating at 90°C for 30 min | Pyrococcus horikoshii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Pyrococcus horikoshii |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme that may be responsible for the production of ATP from AMP formed by ADP-dependent glucokinase and phosphofructokinase reactions in the modified Embden-Meyerhof pathway of Pyrococcus furiosus | Pyrococcus horikoshii |