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4-azido-D-galactose 1-phosphate + UTP
UDP-4-azido-D-galactose + diphosphate
-
-
43% conversion rate
-
?
6-deoxy-D-galactose 1-phosphate + UTP
UDP-6-deoxy-D-galactose + diphosphate
-
-
29% conversion rate
-
?
D-galactose 1-phosphate + dTTP
dTDP-D-galactose + diphosphate
-
-
-
-
?
D-galactose 1-phosphate + dUTP
dUDP-D-galactose + diphosphate
-
-
-
-
?
D-galactose 1-phosphate + TTP
TDP-D-galactose + diphosphate
-
-
-
?
D-galactose 1-phosphate + UTP
UDP-D-galactose + diphosphate
D-galactose 1-phosphate + UTP
UDP-galactose + diphosphate
D-galacturonate 1-phosphate + UTP
UDP-D-galacturonate + diphosphate
-
-
-
?
D-glucose 1-phosphate + TTP
TTP-glucose + diphosphate
-
-
-
r
D-glucose 1-phosphate + UTP
UDP-D-glucose + diphosphate
D-glucose 1-phosphate + UTP
UDP-glucose + diphosphate
D-glucuronic acid 1-phosphate + UTP
UDP-glucuronic acid + diphosphate
-
-
-
?
D-xylose 1-phosphate + UTP
UDP-xylose + diphosphate
diphosphate + UDP-D-glucose
UTP + alpha-D-glucose 1-phosphate
-
-
-
r
diphosphate + UDP-D-glucose
UTP + D-glucose 1-phosphate
-
-
-
r
L-arabinopyranose 1-phosphate + UTP
UDP-L-arabinopyranose + diphosphate
-
-
-
?
L-arabinose 1-phosphate + UTP
UDP-L-arabinose + diphosphate
-
-
-
?
N-acetylglucosamine 1-phosphate + UTP
UDP-N-acetylglucosamine + diphosphate
poorly catalysed reaction
-
-
?
TTP + alpha-D-glucose 1-phosphate
diphosphate + TDP-D-glucose
-
-
-
?
UDP + alpha-D-glucose 1-phosphate
UDP-alpha-D-glucose + diphosphate
UDP-galacturonic acid + diphosphate
galacturonic acid 1-phosphate + UTP
reduced activity in floret extracts depleted for AtUSP by immunprecipitation: only endogenous UDP-galacturonic acid-utilizing pyrophosphorylase activity and final step in myo-inositol oxidation pathway
-
-
?
UDP-monosaccharide + diphosphate
monosaccharide 1-phosphate + UTP
salvage pathway
-
-
r
UTP + a monosaccharide 1-phosphate
diphosphate + UDP-monosaccharide
the enzyme catalyzes the formation of various UDP-sugars at the end of salvage pathways
-
-
?
UTP + alpha-D-galactose 1-phosphate
diphosphate + UDP-alpha-D-galactose
UTP + alpha-D-galactose 1-phosphate
diphosphate + UDP-D-galactose
UTP + alpha-D-galactose 1-phosphate
diphosphate + UDP-galactose
-
-
-
r
UTP + alpha-D-galacturonate
diphosphate + UDP-alpha-D-galacturonate
-
-
-
r
UTP + alpha-D-galacturonate 1-phosphate
diphosphate + UDP-D-galacturonate
-
-
-
?
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-alpha-D-glucose
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-D-glucose
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
r
UTP + alpha-D-glucuronate 1-phosphate
diphosphate + UDP-alpha-D-glucuronate
UTP + alpha-D-glucuronate 1-phosphate
diphosphate + UDP-D-glucuronate
-
-
-
?
UTP + alpha-D-xylose 1-phosphate
diphosphate + UDP-D-xylose
-
-
-
?
UTP + alpha-L-arabinose 1-phosphate
diphosphate + UDP-L-arabinose
-
-
-
?
UTP + arabinose 1-phosphate
diphosphate + UDP-arabinose
70.5% of enzyme activity with glucose 1-phosphate
-
-
r
UTP + beta-L-arabinopyranose
diphosphate + UDP-beta-L-arabinopyranose
-
-
-
r
UTP + D-galactose 1-phosphate
diphosphate + UDP-galactose
UTP + D-galacturonic acid 1-phosphate
diphosphate + UDP-galacturonic acid
-
equilibrium constant is 0.24
-
-
r
UTP + D-glucose 1-phosphate
diphosphate + UDP-glucose
UTP + D-glucuronic acid 1-phosphate
diphosphate + UDP-D-glucuronic acid
71.3% of enzyme activity with glucose 1-phosphate
-
-
r
UTP + D-glucuronic acid 1-phosphate
diphosphate + UDP-glucuronic acid
UTP + D-mannose 1-phosphate
diphosphate + UDP-mannose
UTP + D-xylopyranose 1-phosphate
diphosphate + UDP-D-xylopyranose
-
-
-
r
UTP + D-xylose 1-phosphate
diphosphate + UDP-alpha-D-xylopyranose
UTP + D-xylose 1-phosphate
diphosphate + UDP-D-xylose
-
-
-
ir
UTP + D-xylose 1-phosphate
diphosphate + UDP-xylose
35.9% of enzyme activity with glucose 1-phosphate
-
-
r
UTP + L-arabinose 1-phosphate
diphosphate + UDP-beta-L-arabinopyranose
-
-
-
?
UTP + monosaccharide 1-phosphate
diphosphate + UDP-monosaccharide
-
the enzyme has a critical role in pollen development. The products of the AtUSP reaction can act as precursors for the synthesis of glycolipids, glycoproteins, and cell wall components including pectin and hemicellulose
-
-
?
UTP + N-acetyl-D-glucosamine 1-phosphate
diphosphate + UDP-N-acetylglucosamine
4.3% of enzyme activity with glucose 1-phosphate
-
-
r
UTP + N-acetylglucosamine 1-phosphate
diphosphate + UDP-N-acetylglucosamine
additional information
?
-
D-galactose 1-phosphate + UTP
UDP-D-galactose + diphosphate
-
-
95% conversion rate
-
?
D-galactose 1-phosphate + UTP
UDP-D-galactose + diphosphate
-
-
-
?
D-galactose 1-phosphate + UTP
UDP-D-galactose + diphosphate
-
-
-
?
D-galactose 1-phosphate + UTP
UDP-D-galactose + diphosphate
-
30% of the activity with D-glucose 1-phosphate
-
-
?
D-galactose 1-phosphate + UTP
UDP-galactose + diphosphate
-
-
-
?
D-galactose 1-phosphate + UTP
UDP-galactose + diphosphate
-
-
-
r
D-glucose 1-phosphate + UTP
UDP-D-glucose + diphosphate
-
-
28% conversion rate
-
?
D-glucose 1-phosphate + UTP
UDP-D-glucose + diphosphate
preferred substrates
-
-
?
D-glucose 1-phosphate + UTP
UDP-D-glucose + diphosphate
preferred substrates
-
-
?
D-glucose 1-phosphate + UTP
UDP-D-glucose + diphosphate
-
-
-
?
D-glucose 1-phosphate + UTP
UDP-D-glucose + diphosphate
-
-
-
-
?
D-glucose 1-phosphate + UTP
UDP-glucose + diphosphate
pH 7, 35°C, 2 mM Mg2+
reaction stop by boiling, analysis of products by HPLC (monitoring at 262 nm)
-
r
D-glucose 1-phosphate + UTP
UDP-glucose + diphosphate
-
-
-
r
D-xylose 1-phosphate + UTP
UDP-xylose + diphosphate
-
-
-
?
D-xylose 1-phosphate + UTP
UDP-xylose + diphosphate
-
-
-
r
UDP + alpha-D-glucose 1-phosphate
UDP-alpha-D-glucose + diphosphate
-
-
-
-
r
UDP + alpha-D-glucose 1-phosphate
UDP-alpha-D-glucose + diphosphate
-
-
-
-
r
UTP + alpha-D-galactose 1-phosphate
diphosphate + UDP-alpha-D-galactose
-
-
-
r
UTP + alpha-D-galactose 1-phosphate
diphosphate + UDP-alpha-D-galactose
preferred substrates
-
-
r
UTP + alpha-D-galactose 1-phosphate
diphosphate + UDP-alpha-D-galactose
-
-
-
r
UTP + alpha-D-galactose 1-phosphate
diphosphate + UDP-D-galactose
-
-
-
?
UTP + alpha-D-galactose 1-phosphate
diphosphate + UDP-D-galactose
-
-
-
?
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-alpha-D-glucose
-
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-alpha-D-glucose
-
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-alpha-D-glucose
-
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-D-glucose
-
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-D-glucose
-
-
-
r
UTP + alpha-D-glucuronate 1-phosphate
diphosphate + UDP-alpha-D-glucuronate
-
-
-
r
UTP + alpha-D-glucuronate 1-phosphate
diphosphate + UDP-alpha-D-glucuronate
-
-
-
r
UTP + D-galactose 1-phosphate
diphosphate + UDP-galactose
116% of the activity with glucuronic acid 1-phosphate
-
-
?
UTP + D-galactose 1-phosphate
diphosphate + UDP-galactose
-
-
-
r
UTP + D-galactose 1-phosphate
diphosphate + UDP-galactose
-
-
-
?
UTP + D-galactose 1-phosphate
diphosphate + UDP-galactose
116.2% of enzyme activity with glucose 1-phosphate
-
-
r
UTP + D-galactose 1-phosphate
diphosphate + UDP-galactose
-
3% of the activity with N-acetylglucosamine 1-phosphate
-
-
?
UTP + D-galactose 1-phosphate
diphosphate + UDP-galactose
-
3% of the activity with N-acetylglucosamine 1-phosphate
-
-
?
UTP + D-glucose 1-phosphate
diphosphate + UDP-glucose
114% of the activity with glucuronic acid 1-phosphate
-
-
r
UTP + D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
r
UTP + D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
r
UTP + D-glucose 1-phosphate
diphosphate + UDP-glucose
UTP specific
-
-
r
UTP + D-glucose 1-phosphate
diphosphate + UDP-glucose
-
159% of the activity with N-acetylglucosamine 1-phosphate
-
-
?
UTP + D-glucose 1-phosphate
diphosphate + UDP-glucose
-
159% of the activity with N-acetylglucosamine 1-phosphate
-
-
?
UTP + D-glucuronic acid 1-phosphate
diphosphate + UDP-glucuronic acid
-
-
-
r
UTP + D-glucuronic acid 1-phosphate
diphosphate + UDP-glucuronic acid
-
-
-
?
UTP + D-mannose 1-phosphate
diphosphate + UDP-mannose
-
5% of the activity with N-acetylglucosamine 1-phosphate
-
-
?
UTP + D-mannose 1-phosphate
diphosphate + UDP-mannose
-
5% of the activity with N-acetylglucosamine 1-phosphate
-
-
?
UTP + D-xylose 1-phosphate
diphosphate + UDP-alpha-D-xylopyranose
-
-
-
?
UTP + D-xylose 1-phosphate
diphosphate + UDP-alpha-D-xylopyranose
-
44% of the activity with N-acetylglucosamine 1-phosphate
-
-
?
UTP + D-xylose 1-phosphate
diphosphate + UDP-alpha-D-xylopyranose
-
44% of the activity with N-acetylglucosamine 1-phosphate
-
-
?
UTP + N-acetylglucosamine 1-phosphate
diphosphate + UDP-N-acetylglucosamine
-
-
-
-
?
UTP + N-acetylglucosamine 1-phosphate
diphosphate + UDP-N-acetylglucosamine
-
-
-
-
?
additional information
?
-
no activity detectable for substrates L-fucose 1-phosphate and mannose 1-phosphate
-
-
?
additional information
?
-
enzyme HvUSP has a promiscuous substrate spectrum. It is not active with ATP, CTP, GTP or dTTP, and in combination with UTP HvUSP shows conversion in decreasing order of Gal-1-P, Glc-1-P, GlcA-1-P and Xyl-1-P. No activity with mannose-1phosphate, N-acetyl-glucosamine-1-phosphate, glucosamine-1-phosphate, or fucose-1-phosphate
-
-
-
additional information
?
-
the enzyme preferentially generates UDP-glucose and UDP-galactose, but it may also activate other hexose- or pentose-1-phosphates such as galacturonic acid-1-phosphate or arabinose-1-phosphate
-
-
?
additional information
?
-
the Leishmania major UDP-sugar pyrophosphorylase exhibits a broad substrate specificity in vitro
-
-
?
additional information
?
-
-
no substrate: D-mannose 1-phosphate
-
-
?
additional information
?
-
-
the UDP-sugar pyrophosphorylase of Trypanosoma cruzi shows a broader substrate specificity, the nucleotidyltransferase is capable of transforming different types of sugar 1-phosphates and NTP into NDP-sugars
-
-
?
additional information
?
-
the UDP-sugar pyrophosphorylase of Trypanosoma cruzi shows a broader substrate specificity, the nucleotidyltransferase is capable of transforming different types of sugar 1-phosphates and NTP into NDP-sugars
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
TTP + alpha-D-glucose 1-phosphate
diphosphate + TDP-D-glucose
-
-
-
?
UDP-galacturonic acid + diphosphate
galacturonic acid 1-phosphate + UTP
reduced activity in floret extracts depleted for AtUSP by immunprecipitation: only endogenous UDP-galacturonic acid-utilizing pyrophosphorylase activity and final step in myo-inositol oxidation pathway
-
-
?
UDP-monosaccharide + diphosphate
monosaccharide 1-phosphate + UTP
salvage pathway
-
-
r
UTP + a monosaccharide 1-phosphate
diphosphate + UDP-monosaccharide
the enzyme catalyzes the formation of various UDP-sugars at the end of salvage pathways
-
-
?
UTP + alpha-D-galactose 1-phosphate
diphosphate + UDP-alpha-D-galactose
UTP + alpha-D-galactose 1-phosphate
diphosphate + UDP-D-galactose
-
-
-
?
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-alpha-D-glucose
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-D-glucose
-
-
-
r
UTP + alpha-D-glucuronate 1-phosphate
diphosphate + UDP-alpha-D-glucuronate
UTP + alpha-D-glucuronate 1-phosphate
diphosphate + UDP-D-glucuronate
-
-
-
?
UTP + alpha-D-xylose 1-phosphate
diphosphate + UDP-D-xylose
-
-
-
?
UTP + D-galactose 1-phosphate
diphosphate + UDP-galactose
-
-
-
r
UTP + D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
r
UTP + D-xylose 1-phosphate
diphosphate + UDP-D-xylose
-
-
-
ir
UTP + monosaccharide 1-phosphate
diphosphate + UDP-monosaccharide
-
the enzyme has a critical role in pollen development. The products of the AtUSP reaction can act as precursors for the synthesis of glycolipids, glycoproteins, and cell wall components including pectin and hemicellulose
-
-
?
additional information
?
-
UTP + alpha-D-galactose 1-phosphate
diphosphate + UDP-alpha-D-galactose
-
-
-
r
UTP + alpha-D-galactose 1-phosphate
diphosphate + UDP-alpha-D-galactose
-
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-alpha-D-glucose
-
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-alpha-D-glucose
-
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-alpha-D-glucose
-
-
-
r
UTP + alpha-D-glucuronate 1-phosphate
diphosphate + UDP-alpha-D-glucuronate
-
-
-
r
UTP + alpha-D-glucuronate 1-phosphate
diphosphate + UDP-alpha-D-glucuronate
-
-
-
r
additional information
?
-
the enzyme preferentially generates UDP-glucose and UDP-galactose, but it may also activate other hexose- or pentose-1-phosphates such as galacturonic acid-1-phosphate or arabinose-1-phosphate
-
-
?
additional information
?
-
-
the UDP-sugar pyrophosphorylase of Trypanosoma cruzi shows a broader substrate specificity, the nucleotidyltransferase is capable of transforming different types of sugar 1-phosphates and NTP into NDP-sugars
-
-
?
additional information
?
-
the UDP-sugar pyrophosphorylase of Trypanosoma cruzi shows a broader substrate specificity, the nucleotidyltransferase is capable of transforming different types of sugar 1-phosphates and NTP into NDP-sugars
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
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0.2765 - 1.255
alpha-D-galactose 1-phosphate
0.177 - 0.27
alpha-D-glucose 1-phosphate
0.96
arabinose 1-phosphate
pH 7.0, 35°C
0.09 - 0.86
D-galactose 1-phosphate
1.15
D-galacturonic acid 1-phosphate
-
pH 7.0, 35°C
0.13 - 1.76
D-glucose 1-phosphate
0.094 - 0.48
D-glucuronic acid 1-phosphate
1 - 2.538
D-xylose 1-phosphate
0.134 - 1.018
diphosphate
8.14
dTTP
-
pH not specified in the publication, temperature not specified in the publication
3.74
dUTP
-
pH not specified in the publication, temperature not specified in the publication
0.96 - 1.5
L-arabinose 1-phosphate
0.6
N-acetylglucosamine 1-phosphate
-
pH 8.0, 73°C
0.79
UDP-alpha-D-galacturonate
reverse reaction, pH 7.8, 25°C
0.373
UDP-beta-L-arabinopyranose
reverse reaction, pH 7.8, 25°C
0.148
UDP-D-galactose
reverse reaction, pH 7.8, 25°C
0.79
UDP-D-galacturonate
reverse reaction, pH 7.8, 25°C
0.026 - 0.34
UDP-D-glucose
0.148 - 0.44
UDP-galactose
1.26
UDP-galacturonic acid
-
pH 7.0, 35°C
0.373
UDP-L-arabinopyranose
reverse reaction, pH 7.8, 25°C
additional information
additional information
-
0.2765
alpha-D-galactose 1-phosphate
mutant V330W/F383R, pH and temperature not specified in the publication
0.3
alpha-D-galactose 1-phosphate
pH 7.5, 37°C, recombinant enzyme
0.364
alpha-D-galactose 1-phosphate
mutant F383Rd-type enzyme, pH and temperature not specified in the publication
0.43
alpha-D-galactose 1-phosphate
-
0.529
alpha-D-galactose 1-phosphate
wild-type enzyme, pH and temperature not specified in the publication
1.007
alpha-D-galactose 1-phosphate
mutant F199W, pH and temperature not specified in the publication
1.255
alpha-D-galactose 1-phosphate
mutant V330W, pH and temperature not specified in the publication
0.177
alpha-D-glucose 1-phosphate
pH 7.6, 37°C, recombinant enzyme
0.27
alpha-D-glucose 1-phosphate
-
0.09
D-galactose 1-phosphate
cosubstrate UTP, S0.5 value, pH 8.0, 37°C
0.27
D-galactose 1-phosphate
pH 7, 35°C
0.58
D-galactose 1-phosphate
pH 7.0, 35°C
0.818
D-galactose 1-phosphate
co-substrate: UTP, pH 7.6, 37°C
0.86
D-galactose 1-phosphate
forward reaction, pH 7.8, 25°C
0.13
D-glucose 1-phosphate
cosubstrate UTP, S0.5 value, pH 8.0, 37°C
0.177
D-glucose 1-phosphate
co-substrate: UTP, pH 7.6, 37°C
0.177
D-glucose 1-phosphate
forward reaction, pH 7.6, 37°C
0.23
D-glucose 1-phosphate
pH 8.0
0.23
D-glucose 1-phosphate
pH 7, 35°C
0.34
D-glucose 1-phosphate
pH 7.0, 35°C
0.36
D-glucose 1-phosphate
cosubstrate TTP, S0.5 value, pH 8.0, 37°C
1.49
D-glucose 1-phosphate
-
pH 8.0, 37°C
1.7
D-glucose 1-phosphate
forward reaction, pH 7.8, 25°C
1.706
D-glucose 1-phosphate
forward reaction, pH 7.8, 25°C
1.76
D-glucose 1-phosphate
co-substrate: TTP, pH 7.6, 37°C
0.094
D-glucuronic acid 1-phosphate
pH 7, 35°C
0.14
D-glucuronic acid 1-phosphate
pH 8.0
0.48
D-glucuronic acid 1-phosphate
pH 7.0, 35°C
1
D-xylose 1-phosphate
pH 7, 35°C
1.98
D-xylose 1-phosphate
pH 7.0, 35°C
2.538
D-xylose 1-phosphate
co-substrate: UTP, pH 7.6, 37°C
0.134
diphosphate
reverse reaction, pH 7.6, 37°C
0.136
diphosphate
pH 7.6, 37°C, recombinant enzyme
0.16
diphosphate
pH 7, 35°C
0.25
diphosphate
pH 7.0, 35°C
0.307
diphosphate
reverse reaction, UDP-galactose, pH 7.8, 25°C
0.307
diphosphate
reverse reaction, co-substrate: UDP-D-galactose, pH 7.8, 25°C
0.383
diphosphate
reverse reaction, UDP-glucose, pH 7.8, 25°C
0.383
diphosphate
reverse reaction, co-substrate: UDP-D-glucose, pH 7.8, 25°C
0.7
diphosphate
-
pH 7.0, 35°C
0.728
diphosphate
reverse reaction, UDP-alpha-D-galacturonate, pH 7.8, 25°C
0.728
diphosphate
reverse reaction, co-substrate: UDP-D-galacturonate, pH 7.8, 25°C
1.01
diphosphate
reverse reaction, co-substrate: UDP-L-arabinopyranose, pH 7.8, 25°C
1.018
diphosphate
reverse reaction, UDP-beta-L-arabinopyranose, pH 7.8, 25°C
0.96
L-arabinose 1-phosphate
pH 7.0, 35°C
1.5
L-arabinose 1-phosphate
pH 7, 35°C
0.9
TTP
cosubstrate D-glucose 1-phosphate, S0.5 value, pH 8.0, 37°C
2.54
TTP
co-substrate: glucose 1-phosphate, pH 7.6, 37°C
0.026
UDP-D-glucose
pH 7.6, 37°C, recombinant enzyme
0.174
UDP-D-glucose
reverse reaction, pH 7.8, 25°C
0.34
UDP-D-glucose
pH 7.0, 35°C
0.148
UDP-galactose
reverse reaction, pH 7.8, 25°C
0.026
UDP-glucose
reverse reaction, pH 7.6, 37°C
0.174
UDP-glucose
reverse reaction, pH 7.8, 25°C
0.28
UDP-glucose
pH 7, 35°C
0.34
UDP-glucose
pH 7.0, 35°C
0.0284
UTP
pH 7.6, 37°C, recombinant enzyme
0.0284
UTP
forward reaction, pH 7.6, 37°C
0.048
UTP
D-glucose 1-phosphate, pH 7.0, 35°C
0.08
UTP
cosubstrate D-galactose 1-phosphate, S0.5 value, pH 8.0, 37°C
0.098
UTP
forward reaction, D-galactose 1-phosphate, pH 7.8, 25°C
0.098
UTP
forward reaction, co-substrate: D-galactose 1-phosphate, pH 7.8, 25°C
0.116
UTP
forward reaction, D-glucose 1-phosphate, pH 7.8, 25°C
0.116
UTP
forward reaction, co-substrate: D-glucose 1-phosphate, pH 7.8, 25°C
0.14
UTP
cosubstrate D-glucose 1-phosphate, S0.5 value, pH 8.0, 37°C
0.15
UTP
pH 8.0, cosubstrate: glucuronic acid 1-phosphate
0.19
UTP
pH 8.0, cosubstrate: glucose 1-phosphate
2.88
UTP
-
pH not specified in the publication, temperature not specified in the publication
additional information
additional information
Michaelis-Menten kinetics
-
additional information
additional information
Michaelis-Menten kinetics
-
additional information
additional information
-
kinetics, overview
-
additional information
additional information
kinetics, overview
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
17.33 - 319.32
alpha-D-galactose 1-phosphate
35.2 - 219
D-galactose 1-phosphate
0.008 - 191
D-glucose 1-phosphate
67
D-glucuronic acid 1-phosphate
pH 7, 35°C
8.6 - 51
D-xylose 1-phosphate
27
dTTP
-
pH not specified in the publication, temperature not specified in the publication
219
dUTP
-
pH not specified in the publication, temperature not specified in the publication
138
L-arabinose 1-phosphate
pH 7, 35°C
0.2
N-acetylglucosamine 1-phosphate
-
pH 8.0, 73°C
0.085
TTP
co-substrate: glucose 1-phosphate, pH 7.6, 37°C
13
UDP-alpha-D-galacturonate
reverse reaction, pH 7.8, 25°C
72
UDP-beta-L-arabinopyranose
reverse reaction, pH 7.8, 25°C
154
UDP-D-galactose
reverse reaction, pH 7.8, 25°C
13
UDP-D-galacturonate
reverse reaction, pH 7.8, 25°C
180
UDP-D-glucose
reverse reaction, pH 7.8, 25°C
154
UDP-galactose
reverse reaction, pH 7.8, 25°C
72
UDP-L-arabinopyranose
reverse reaction, pH 7.8, 25°C
additional information
D-galactose 1-phosphate
17.33
alpha-D-galactose 1-phosphate
mutant F383Rd-type enzyme, pH and temperature not specified in the publication
25.58
alpha-D-galactose 1-phosphate
mutant V330W/F383R, pH and temperature not specified in the publication
90.25
alpha-D-galactose 1-phosphate
mutant F199W, pH and temperature not specified in the publication
216.45
alpha-D-galactose 1-phosphate
wild-type enzyme, pH and temperature not specified in the publication
319.32
alpha-D-galactose 1-phosphate
mutant V330W, pH and temperature not specified in the publication
35.2
D-galactose 1-phosphate
co-substrate: UTP, pH 7.6, 37°C
133
D-galactose 1-phosphate
pH 7, 35°C
219
D-galactose 1-phosphate
forward reaction, pH 7.8, 25°C
0.008
D-glucose 1-phosphate
-
pH 8.0, 37°C
0.12
D-glucose 1-phosphate
co-substrate: TTP, pH 7.6, 37°C
26
D-glucose 1-phosphate
co-substrate: UTP, pH 7.6, 37°C
122
D-glucose 1-phosphate
pH 7, 35°C
191
D-glucose 1-phosphate
forward reaction, pH 7.8, 25°C
8.6
D-xylose 1-phosphate
co-substrate: UTP, pH 7.6, 37°C
51
D-xylose 1-phosphate
pH 7, 35°C
15
diphosphate
reverse reaction, UDP-alpha-D-galacturonate, pH 7.8, 25°C
15
diphosphate
reverse reaction, co-substrate: UDP-D-galacturonate, pH 7.8, 25°C
81
diphosphate
reverse reaction, UDP-beta-L-arabinopyranose, pH 7.8, 25°C
81
diphosphate
reverse reaction, co-substrate: UDP-L-arabinopyranose, pH 7.8, 25°C
138
diphosphate
pH 7, 35°C
174
diphosphate
reverse reaction, UDP-galactose, pH 7.8, 25°C
174
diphosphate
reverse reaction, co-substrate: UDP-D-galactose, pH 7.8, 25°C
185
diphosphate
reverse reaction, UDP-glucose, pH 7.8, 25°C
185
diphosphate
reverse reaction, co-substrate: UDP-D-glucose, pH 7.8, 25°C
133
UDP-glucose
pH 7, 35°C
180
UDP-glucose
reverse reaction, pH 7.8, 25°C
0.003
UTP
-
pH 8.0, 37°C
201
UTP
forward reaction, D-galactose 1-phosphate, pH 7.8, 25°C
201
UTP
forward reaction, co-substrate: D-galactose 1-phosphate, pH 7.8, 25°C
209
UTP
forward reaction, D-glucose 1-phosphate, pH 7.8, 25°C
209
UTP
forward reaction, co-substrate: D-glucose 1-phosphate, pH 7.8, 25°C
348
UTP
-
pH not specified in the publication, temperature not specified in the publication
additional information
D-galactose 1-phosphate
Kcat/KM: 490000 1/M/sec, pH 7, 35°C
additional information
D-glucose 1-phosphate
Kcat/KM: 530000 1/M/sec, pH 7, 35°C
additional information
D-glucuronic acid 1-phosphate
Kcat/KM: 710000 1/M/sec, pH 7, 35°C
additional information
D-xylose 1-phosphate
Kcat/KM: 51000 1/M/sec, pH 7, 35°C
additional information
diphosphate
Kcat/KM: 860000 1/M/sec, pH 7, 35°C
additional information
L-arabinose 1-phosphate
Kcat/KM: 92000 1/M/sec, pH 7, 35°C
additional information
UDP-glucose
Kcat/KM: 480000 1/M/sec, pH 7, 35°C
additional information
UTP
Kcat/KM: 1400000 1/M/sec, pH 7, 35°C
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47.61 - 409.17
alpha-D-galactose 1-phosphate
40 - 259
D-galactose 1-phosphate
0.0054 - 150
D-glucose 1-phosphate
34
D-xylose 1-phosphate
co-substrate: UTP, pH 7.6, 37°C
3.3
dTTP
-
pH not specified in the publication, temperature not specified in the publication
59
dUTP
-
pH not specified in the publication, temperature not specified in the publication
0.0334
TTP
co-substrate: glucose 1-phosphate, pH 7.6, 37°C
1070
UDP-D-galactose
reverse reaction, pH 7.8, 25°C
16.7
UDP-D-galacturonate
reverse reaction, pH 7.8, 25°C
1060
UDP-D-glucose
reverse reaction, pH 7.8, 25°C
1150
UDP-glucose
reverse reaction, pH 7.6, 37°C
198
UDP-L-arabinopyranose
reverse reaction, pH 7.8, 25°C
47.61
alpha-D-galactose 1-phosphate
mutant F383Rd-type enzyme, pH and temperature not specified in the publication
89.62
alpha-D-galactose 1-phosphate
mutant F199W, pH and temperature not specified in the publication
92.51
alpha-D-galactose 1-phosphate
mutant V330W/F383R, pH and temperature not specified in the publication
254.44
alpha-D-galactose 1-phosphate
mutant V330W, pH and temperature not specified in the publication
409.17
alpha-D-galactose 1-phosphate
wild-type enzyme, pH and temperature not specified in the publication
40
D-galactose 1-phosphate
co-substrate: UTP, pH 7.6, 37°C
259
D-galactose 1-phosphate
forward reaction, pH 7.8, 25°C
0.0054
D-glucose 1-phosphate
-
pH 8.0, 37°C
0.068
D-glucose 1-phosphate
co-substrate: TTP, pH 7.6, 37°C
114
D-glucose 1-phosphate
forward reaction, pH 7.8, 25°C
150
D-glucose 1-phosphate
co-substrate: UTP, pH 7.6, 37°C
150
D-glucose 1-phosphate
forward reaction, pH 7.6, 37°C
20.8
diphosphate
reverse reaction, co-substrate: UDP-D-galacturonate, pH 7.8, 25°C
81.2
diphosphate
reverse reaction, co-substrate: UDP-L-arabinopyranose, pH 7.8, 25°C
220
diphosphate
reverse reaction, pH 7.6, 37°C
492
diphosphate
reverse reaction, co-substrate: UDP-D-glucose, pH 7.8, 25°C
580
diphosphate
reverse reaction, co-substrate: UDP-D-galactose, pH 7.8, 25°C
0.0015
UTP
-
pH 8.0, 37°C
121
UTP
-
pH not specified in the publication, temperature not specified in the publication
920
UTP
forward reaction, pH 7.6, 37°C
1830
UTP
forward reaction, co-substrate: D-glucose 1-phosphate, pH 7.8, 25°C
2090
UTP
forward reaction, co-substrate: D-galactose 1-phosphate, pH 7.8, 25°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
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1.2
+/-0.08 micromol/min/mg, N-acetylglucosamine 1-phosphate
22.8
+/-1.1 micromol/min/mg, D-xylose 1-phosphate
223
with UDP-glucose as a substrate
233
with alpha-D-galactose 1-phosphate as a substrate
30
UDP-L-arabinose pyrophosphorylase, pH 7.0, 35°C
410
with alpha-D-glucose 1-phosphate as a substrate
44.5
+/-0.9 micromol/min/mg, L-arabinose 1-phosphate
51.4
+/-3.6 micromol/min/mg, D-glucuronic acid 1-phosphate
678
with UDP-galactose as a substrate
84.3
+/-2.4 micromol/min/mg, D-glucose 1-phosphate
86.9
+/-2.4 micromol/min/mg, D-galactose 1-phosphate
95
recombinant UDP-glucose pyrophosphorylase, pH 7.0, 35°C
63.9
-
63.9
UDP-D-glucose pyrophosphorylase, pH 7.0, 35°C
additional information
143-255% UDP-L-arabinose synthesising activity in extracts from transgenic plants (aerial parts) compared to wild-type plant
additional information
25% of wild-type UDP-L-arabinose synthesising activity in plant extracts (aerial parts) upon repression of AtUSP gene expression
additional information
no activity detectable for substrates L-fucose 1-phosphate and mannose 1-phosphate
additional information
relative activity, alpha-D-galacturonate 1-phosphate, 1.6%, forward reaction, + UTP, pH 7.8, 25°C
additional information
relative activity, alpha-D-galacturonate 1-phosphate, 1.6%, forward reaction, + UTP, pH 7.8, 25°C
additional information
relative activity, D-galactose 1-phosphate, 100%, forward reaction, + UTP, pH 7.8, 25°C
additional information
relative activity, D-galactose 1-phosphate, 100%, forward reaction, + UTP, pH 7.8, 25°C
additional information
relative activity, D-glucose 1-phosphate, 88%, forward reaction, + UTP, pH 7.8, 25°C
additional information
relative activity, D-glucose 1-phosphate, 88%, forward reaction, + UTP, pH 7.8, 25°C
additional information
relative activity, D-xylopyranose 1-phosphate, 12%, forward reaction, + UTP, pH 7.8, 25°C
additional information
relative activity, D-xylopyranose 1-phosphate, 12%, forward reaction, + UTP, pH 7.8, 25°C
additional information
relative activity, UDP-alpha-D-galacturonate, 7.1%, reverse reaction, + diphosphate, pH 7.8, 25°C
additional information
relative activity, UDP-alpha-D-galacturonate, 7.1%, reverse reaction, + diphosphate, pH 7.8, 25°C
additional information
relative activity, UDP-beta-L-arabinopyranose, 33%, reverse reaction, + diphosphate, pH 7.8, 25°C
additional information
relative activity, UDP-beta-L-arabinopyranose, 33%, reverse reaction, + diphosphate, pH 7.8, 25°C
additional information
relative activity, UDP-galactose, 83%, reverse reaction, + diphosphate, pH 7.8, 25°C
additional information
relative activity, UDP-galactose, 83%, reverse reaction, + diphosphate, pH 7.8, 25°C
additional information
relative activity, UDP-glucose, 100%, reverse reaction, + diphosphate, pH 7.8, 25°C
additional information
relative activity, UDP-glucose, 100%, reverse reaction, + diphosphate, pH 7.8, 25°C
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Ohashi, T.; Cramer, N.; Ishimizu, T.; Hase, S.
Preparation of UDP-galacturonic acid using UDP-sugar pyrophosphorylase
Anal. Biochem.
352
182-187
2006
Pisum sativum
brenda
Kim, J.S.; Koh, S.; Shin, H.J.; Lee, D.S.; Lee, S.Y.
Biochemical characterization of a UDP-sugar pyrophosphorylase from Thermus caldophilus GK24
Biotechnol. Appl. Biochem.
29
11-17
1999
Thermus caldophilus, Thermus caldophilus GK24
brenda
Kotake, T.; Yamaguchi, D.; Ohzono, H.; Hojo, S.; Kaneko, S.; Ishida, H.K.; Tsumuraya, Y.
UDP-sugar pyrophosphorylase with broad substrate specificity toward various monosaccharide 1-phosphates from pea sprouts
J. Biol. Chem.
279
45728-45736
2004
Pisum sativum (Q5W915), Pisum sativum
brenda
Litterer, L.A.; Plaisance, K.L.; Schnurr, J.A.; Storey, K.K.; Jung, H.J.G.; Gronwald, J.W.; Somers, D.A.
Biosynthesis of UDP-glucuronic acid in developing soybean embryos: Possible role of UDP-sugar pyrophosphorylase
Physiol. Plant.
128
200-211
2006
Glycine max (Q09WE7)
brenda
Schnurr, J.A.; Storey, K.K.; Jung, H.J.; Somers, D.A.; Gronwald, J.W.
UDP-sugar pyrophosphorylase is essential for pollen development in Arabidopsis
Planta
224
520-532
2006
Arabidopsis thaliana
brenda
Dai, N.; Petreikov, M.; Portnoy, V.; Katzir, N.; Pharr, D.M.; Schaffer, A.A.
Cloning and expression analysis of a UDP-galactose/glucose pyrophosphorylase from melon fruit provides evidence for the major metabolic pathway of galactose metabolism in raffinose oligosaccharide metabolizing plants
Plant Physiol.
142
294-304
2006
Cucumis melo (Q0GZS3)
brenda
Kotake, T.; Hojo, S.; Yamaguchi, D.; Aohara, T.; Konishi, T.; Tsumuraya, Y.
Properties and physiological functions of UDP-sugar pyrophosphorylase in Arabidopsis
Biosci. Biotechnol. Biochem.
71
761-771
2007
Arabidopsis thaliana (Q9C5I1)
brenda
Gronwald, J.W.; Miller, S.S.; Vance, C.P.
Arabidopsis UDP-sugar pyrophosphorylase: Evidence for two isoforms
Plant Physiol. Biochem.
46
1101-1105
2008
Arabidopsis thaliana (Q9C5I1)
brenda
Siddique, S.; Endres, S.; Atkins, J.M.; Szakasits, D.; Wieczorek, K.; Hofmann, J.; Blaukopf, C.; Urwin, P.E.; Tenhaken, R.; Grundler, F.M.; Kreil, D.P.; Bohlmann, H.
Myo-inositol oxygenase genes are involved in the development of syncytia induced by Heterodera schachtii in Arabidopsis roots
New Phytol.
184
457-472
2009
Arabidopsis thaliana (Q9C5I1)
brenda
Damerow, S.; Lamerz, A.C.; Haselhorst, T.; Fuehring, J.; Zarnovican, P.; von Itzstein, M.; Routier, F.H.
Leishmania UDP-sugar pyrophosphorylase: the missing link in galactose salvage?
J. Biol. Chem.
285
878-887
2010
Leishmania major (D3G6S4), Leishmania major (Q4QE75)
brenda
Muthana, M.M.; Qu, J.; Li, Y.; Zhang, L.; Yu, H.; Ding, L.; Malekan, H.; Chen, X.
Efficient one-pot multienzyme synthesis of UDP-sugars using a promiscuous UDP-sugar pyrophosphorylase from Bifidobacterium longum (BLUSP)
Chem. Commun. (Camb. )
48
2728-2730
2012
Bifidobacterium longum, Bifidobacterium longum ATCC 55813
brenda
Yang, T.; Bar-Peled, M.
Identification of a novel UDP-sugar pyrophosphorylase with a broad substrate specificity in Trypanosoma cruzi
Biochem. J.
429
533-543
2010
Trypanosoma cruzi, Trypanosoma cruzi (D3YBH2)
brenda
Dickmanns, A.; Damerow, S.; Neumann, P.; Schulz, E.C.; Lamerz, A.C.; Routier, F.H.; Ficner, R.
Structural basis for the broad substrate range of the UDP-sugar pyrophosphorylase from Leishmania major
J. Mol. Biol.
405
461-478
2011
no activity in Homo sapiens, Leishmania major (D3G6S4)
brenda
Geserick, C.; Tenhaken, R.
UDP-sugar pyrophosphorylase is essential for arabinose and xylose recycling, and is required during vegetative and reproductive growth in Arabidopsis
Plant J.
74
239-247
2013
Arabidopsis thaliana
brenda
Ebrecht, A.C.; Asencion Diez, M.D.; Piattoni, C.V.; Guerrero, S.A.; Iglesias, A.A.
The UDP-glucose pyrophosphorylase from Giardia lamblia is redox regulated and exhibits promiscuity to use galactose-1-phosphate
Biochim. Biophys. Acta
1850
88-96
2015
Giardia intestinalis (A8BJJ7), Giardia intestinalis ATCC 50803 (A8BJJ7)
brenda
Liu, J.; Zou, Y.; Guan, W.; Zhai, Y.; Xue, M.; Jin, L.; Zhao, X.; Dong, J.; Wang, W.; Shen, J.; Wang, P.G.; Chen, M.
Biosynthesis of nucleotide sugars by a promiscuous UDP-sugar pyrophosphorylase from Arabidopsis thaliana (AtUSP)
Bioorg. Med. Chem. Lett.
23
3764-3768
2013
Arabidopsis thaliana
brenda
Kim, B.; Ahn, J.
Characterization of uridine diphosphate-sugar pyrophosphorylase from Populus deltoids
J. Korean Soc. Appl. Biol. Chem.
56
525-531
2013
Populus deltoides
-
brenda
Geserick, C.; Tenhaken, R.
UDP-sugar pyrophosphorylase controls the activity of proceeding sugar-1-kinases enzymes
Plant Signal. Behav.
8
e25478
2013
Arabidopsis thaliana (Q9C5I1)
brenda
Zang, L.; Du, R.; Zang, H.; Wang, F.; Sheng, J.
Production of Arabidopsis thaliana UDP-sugar pyrophosphorylase by Pichia pastoris and its application in efficient UDP-Glucose and UDP-glucuronic acid synthesis
Appl. Biochem. Microbiol.
55
631-638
2019
Arabidopsis thaliana (Q9C5I1)
-
brenda
Wahl, C.; Spiertz, M.; Elling, L.
Characterization of a new UDP-sugar pyrophosphorylase from Hordeum vulgare (barley)
J. Biotechnol.
258
51-55
2017
Hordeum vulgare subsp. vulgare (F2DQG9)
brenda
Prakash, O.; Fuehring, J.; Post, J.; Shepherd, S.M.; Eadsforth, T.C.; Gray, D.; Fedorov, R.; Routier, F.H.
Identification of Leishmania major UDP-sugar pyrophosphorylase inhibitors using biosensor-based small molecule fragment library screening
Molecules
24
996
2019
Leishmania major (Q4QE75)
brenda