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Information on EC 2.7.7.64 - UTP-monosaccharide-1-phosphate uridylyltransferase
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EC Tree
2.7.7.64 UTP-monosaccharide-1-phosphate uridylyltransferaseSpecify your search results
Word Map
- 2.7.7.64
- udp-sugars
-
gal-1-p
- synthesis
- udp-glucose
- udp-glca
- 1-phosphates
-
co-substrate
-
phytohormones
- l-arabinose
- glycolipids
-
three-enzyme
-
pisum
- sativum
- molecular biology
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
atusp, psusp, uspase, udp-galactose/glucose pyrophosphorylase, 
more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
AtUSP
GL50803_29307
UDP-sugar pyrophosphorylase
USP
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
UTP + a monosaccharide 1-phosphate = diphosphate + UDP-monosaccharide
-
-
-
-
UTP + a monosaccharide 1-phosphate = diphosphate + UDP-monosaccharide
UDP-sugar-producing pyrophosphorylases catalyze reversible reactions, require magnesium for catalysis, and act through an ordered sequential Bi-Bi reaction mechanism. In the forward reaction, UTP binds before the sugar 1-phosphate, and after catalysis, inorganic diphosphate is released before the UDP-sugar
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PATHWAY SOURCE
PATHWAYS
KEGG
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CAS REGISTRY NUMBER
COMMENTARY
223918-15-8
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
4-azido-D-galactose 1-phosphate + UTP
UDP-4-azido-D-galactose + diphosphate
-
-
43% conversion rate
-
?
6-deoxy-D-galactose 1-phosphate + UTP
UDP-6-deoxy-D-galactose + diphosphate
-
-
29% conversion rate
-
?
D-galactose 1-phosphate + TTP
TDP-D-galactose + diphosphate
-
-
-
?
D-galacturonate 1-phosphate + UTP
UDP-D-galacturonate + diphosphate
-
-
-
?
D-glucuronic acid 1-phosphate + UTP
UDP-glucuronic acid + diphosphate
-
-
-
?
diphosphate + UDP-D-glucose
UTP + alpha-D-glucose 1-phosphate
-
-
-
r
L-arabinopyranose 1-phosphate + UTP
UDP-L-arabinopyranose + diphosphate
-
-
-
?
L-arabinose 1-phosphate + UTP
UDP-L-arabinose + diphosphate
-
-
-
?
N-acetylglucosamine 1-phosphate + UTP
UDP-N-acetylglucosamine + diphosphate
poorly catalysed reaction
-
-
?
TTP + alpha-D-glucose 1-phosphate
diphosphate + TDP-D-glucose
-
-
-
?
UDP-galacturonic acid + diphosphate
galacturonic acid 1-phosphate + UTP
reduced activity in floret extracts depleted for AtUSP by immunprecipitation: only endogenous UDP-galacturonic acid-utilizing pyrophosphorylase activity and final step in myo-inositol oxidation pathway
-
-
?
UDP-monosaccharide + diphosphate
monosaccharide 1-phosphate + UTP
salvage pathway
-
-
r
UTP + a monosaccharide 1-phosphate
diphosphate + UDP-monosaccharide
the enzyme catalyzes the formation of various UDP-sugars at the end of salvage pathways
-
-
?
UTP + alpha-D-galacturonate
diphosphate + UDP-alpha-D-galacturonate
-
-
-
r
UTP + alpha-D-galacturonate 1-phosphate
diphosphate + UDP-D-galacturonate
-
-
-
?
UTP + alpha-D-glucuronate 1-phosphate
diphosphate + UDP-D-glucuronate
-
-
-
?
UTP + alpha-L-arabinose 1-phosphate
diphosphate + UDP-L-arabinose
-
-
-
?
UTP + arabinose 1-phosphate
diphosphate + UDP-arabinose
70.5% of enzyme activity with glucose 1-phosphate
-
-
r
UTP + beta-L-arabinopyranose
diphosphate + UDP-beta-L-arabinopyranose
-
-
-
r
UTP + D-galacturonic acid 1-phosphate
diphosphate + UDP-galacturonic acid
-
equilibrium constant is 0.24
-
-
r
UTP + D-glucuronic acid 1-phosphate
diphosphate + UDP-D-glucuronic acid
71.3% of enzyme activity with glucose 1-phosphate
-
-
r
UTP + D-xylopyranose 1-phosphate
diphosphate + UDP-D-xylopyranose
-
-
-
r
UTP + D-xylose 1-phosphate
diphosphate + UDP-D-xylose
-
-
-
ir
UTP + D-xylose 1-phosphate
diphosphate + UDP-xylose
35.9% of enzyme activity with glucose 1-phosphate
-
-
r
UTP + L-arabinose 1-phosphate
diphosphate + UDP-beta-L-arabinopyranose
-
-
-
?
UTP + monosaccharide 1-phosphate
diphosphate + UDP-monosaccharide
-
the enzyme has a critical role in pollen development. The products of the AtUSP reaction can act as precursors for the synthesis of glycolipids, glycoproteins, and cell wall components including pectin and hemicellulose
-
-
?
UTP + N-acetyl-D-glucosamine 1-phosphate
diphosphate + UDP-N-acetylglucosamine
4.3% of enzyme activity with glucose 1-phosphate
-
-
r
D-galactose 1-phosphate + UTP
UDP-D-galactose + diphosphate
-
-
95% conversion rate
-
?
D-galactose 1-phosphate + UTP
UDP-D-galactose + diphosphate
-
-
-
?
D-galactose 1-phosphate + UTP
UDP-D-galactose + diphosphate
-
-
-
?
D-galactose 1-phosphate + UTP
UDP-D-galactose + diphosphate
-
30% of the activity with D-glucose 1-phosphate
-
-
?
D-galactose 1-phosphate + UTP
UDP-galactose + diphosphate
-
-
-
?
D-glucose 1-phosphate + UTP
UDP-D-glucose + diphosphate
-
-
28% conversion rate
-
?
D-glucose 1-phosphate + UTP
UDP-D-glucose + diphosphate
preferred substrates
-
-
?
D-glucose 1-phosphate + UTP
UDP-D-glucose + diphosphate
preferred substrates
-
-
?
D-glucose 1-phosphate + UTP
UDP-D-glucose + diphosphate
-
-
-
?
D-glucose 1-phosphate + UTP
UDP-glucose + diphosphate
pH 7, 35°C, 2 mM Mg2+
reaction stop by boiling, analysis of products by HPLC (monitoring at 262 nm)
-
r
UDP + alpha-D-glucose 1-phosphate
UDP-alpha-D-glucose + diphosphate
-
-
-
-
r
UDP + alpha-D-glucose 1-phosphate
UDP-alpha-D-glucose + diphosphate
-
-
-
-
r
UTP + alpha-D-galactose 1-phosphate
diphosphate + UDP-alpha-D-galactose
-
-
-
r
UTP + alpha-D-galactose 1-phosphate
diphosphate + UDP-alpha-D-galactose
preferred substrates
-
-
r
UTP + alpha-D-galactose 1-phosphate
diphosphate + UDP-alpha-D-galactose
-
-
-
r
UTP + alpha-D-galactose 1-phosphate
diphosphate + UDP-D-galactose
-
-
-
?
UTP + alpha-D-galactose 1-phosphate
diphosphate + UDP-D-galactose
-
-
-
?
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-alpha-D-glucose
-
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-alpha-D-glucose
-
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-alpha-D-glucose
-
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-D-glucose
-
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-D-glucose
-
-
-
r
UTP + alpha-D-glucuronate 1-phosphate
diphosphate + UDP-alpha-D-glucuronate
-
-
-
r
UTP + alpha-D-glucuronate 1-phosphate
diphosphate + UDP-alpha-D-glucuronate
-
-
-
r
UTP + D-galactose 1-phosphate
diphosphate + UDP-galactose
116% of the activity with glucuronic acid 1-phosphate
-
-
?
UTP + D-galactose 1-phosphate
diphosphate + UDP-galactose
-
-
-
r
UTP + D-galactose 1-phosphate
diphosphate + UDP-galactose
116.2% of enzyme activity with glucose 1-phosphate
-
-
r
UTP + D-galactose 1-phosphate
diphosphate + UDP-galactose
-
3% of the activity with N-acetylglucosamine 1-phosphate
-
-
?
UTP + D-galactose 1-phosphate
diphosphate + UDP-galactose
-
3% of the activity with N-acetylglucosamine 1-phosphate
-
-
?
UTP + D-glucose 1-phosphate
diphosphate + UDP-glucose
114% of the activity with glucuronic acid 1-phosphate
-
-
r
UTP + D-glucose 1-phosphate
diphosphate + UDP-glucose
UTP specific
-
-
r
UTP + D-glucose 1-phosphate
diphosphate + UDP-glucose
-
159% of the activity with N-acetylglucosamine 1-phosphate
-
-
?
UTP + D-glucose 1-phosphate
diphosphate + UDP-glucose
-
159% of the activity with N-acetylglucosamine 1-phosphate
-
-
?
UTP + D-glucuronic acid 1-phosphate
diphosphate + UDP-glucuronic acid
-
-
-
r
UTP + D-glucuronic acid 1-phosphate
diphosphate + UDP-glucuronic acid
-
-
-
?
UTP + D-mannose 1-phosphate
diphosphate + UDP-mannose
-
5% of the activity with N-acetylglucosamine 1-phosphate
-
-
?
UTP + D-mannose 1-phosphate
diphosphate + UDP-mannose
-
5% of the activity with N-acetylglucosamine 1-phosphate
-
-
?
UTP + D-xylose 1-phosphate
diphosphate + UDP-alpha-D-xylopyranose
-
-
-
?
UTP + D-xylose 1-phosphate
diphosphate + UDP-alpha-D-xylopyranose
-
44% of the activity with N-acetylglucosamine 1-phosphate
-
-
?
UTP + D-xylose 1-phosphate
diphosphate + UDP-alpha-D-xylopyranose
-
44% of the activity with N-acetylglucosamine 1-phosphate
-
-
?
UTP + N-acetylglucosamine 1-phosphate
diphosphate + UDP-N-acetylglucosamine
-
-
-
-
?
UTP + N-acetylglucosamine 1-phosphate
diphosphate + UDP-N-acetylglucosamine
-
-
-
-
?
additional information
?
-
no activity detectable for substrates L-fucose 1-phosphate and mannose 1-phosphate
-
-
?
additional information
?
-
enzyme HvUSP has a promiscuous substrate spectrum. It is not active with ATP, CTP, GTP or dTTP, and in combination with UTP HvUSP shows conversion in decreasing order of Gal-1-P, Glc-1-P, GlcA-1-P and Xyl-1-P. No activity with mannose-1phosphate, N-acetyl-glucosamine-1-phosphate, glucosamine-1-phosphate, or fucose-1-phosphate
-
-
-
additional information
?
-
the enzyme preferentially generates UDP-glucose and UDP-galactose, but it may also activate other hexose- or pentose-1-phosphates such as galacturonic acid-1-phosphate or arabinose-1-phosphate
-
-
?
additional information
?
-
the Leishmania major UDP-sugar pyrophosphorylase exhibits a broad substrate specificity in vitro
-
-
?
additional information
?
-
-
the UDP-sugar pyrophosphorylase of Trypanosoma cruzi shows a broader substrate specificity, the nucleotidyltransferase is capable of transforming different types of sugar 1-phosphates and NTP into NDP-sugars
-
-
?
additional information
?
-
the UDP-sugar pyrophosphorylase of Trypanosoma cruzi shows a broader substrate specificity, the nucleotidyltransferase is capable of transforming different types of sugar 1-phosphates and NTP into NDP-sugars
-
-
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
TTP + alpha-D-glucose 1-phosphate
diphosphate + TDP-D-glucose
-
-
-
?
UDP-galacturonic acid + diphosphate
galacturonic acid 1-phosphate + UTP
reduced activity in floret extracts depleted for AtUSP by immunprecipitation: only endogenous UDP-galacturonic acid-utilizing pyrophosphorylase activity and final step in myo-inositol oxidation pathway
-
-
?
UDP-monosaccharide + diphosphate
monosaccharide 1-phosphate + UTP
salvage pathway
-
-
r
UTP + a monosaccharide 1-phosphate
diphosphate + UDP-monosaccharide
the enzyme catalyzes the formation of various UDP-sugars at the end of salvage pathways
-
-
?
UTP + alpha-D-galactose 1-phosphate
diphosphate + UDP-D-galactose
-
-
-
?
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-D-glucose
-
-
-
r
UTP + alpha-D-glucuronate 1-phosphate
diphosphate + UDP-D-glucuronate
-
-
-
?
UTP + D-galactose 1-phosphate
diphosphate + UDP-galactose
-
-
-
r
UTP + D-xylose 1-phosphate
diphosphate + UDP-D-xylose
-
-
-
ir
UTP + monosaccharide 1-phosphate
diphosphate + UDP-monosaccharide
-
the enzyme has a critical role in pollen development. The products of the AtUSP reaction can act as precursors for the synthesis of glycolipids, glycoproteins, and cell wall components including pectin and hemicellulose
-
-
?
UTP + alpha-D-galactose 1-phosphate
diphosphate + UDP-alpha-D-galactose
-
-
-
r
UTP + alpha-D-galactose 1-phosphate
diphosphate + UDP-alpha-D-galactose
-
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-alpha-D-glucose
-
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-alpha-D-glucose
-
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-alpha-D-glucose
-
-
-
r
UTP + alpha-D-glucuronate 1-phosphate
diphosphate + UDP-alpha-D-glucuronate
-
-
-
r
UTP + alpha-D-glucuronate 1-phosphate
diphosphate + UDP-alpha-D-glucuronate
-
-
-
r
additional information
?
-
the enzyme preferentially generates UDP-glucose and UDP-galactose, but it may also activate other hexose- or pentose-1-phosphates such as galacturonic acid-1-phosphate or arabinose-1-phosphate
-
-
?
additional information
?
-
-
the UDP-sugar pyrophosphorylase of Trypanosoma cruzi shows a broader substrate specificity, the nucleotidyltransferase is capable of transforming different types of sugar 1-phosphates and NTP into NDP-sugars
-
-
?
additional information
?
-
the UDP-sugar pyrophosphorylase of Trypanosoma cruzi shows a broader substrate specificity, the nucleotidyltransferase is capable of transforming different types of sugar 1-phosphates and NTP into NDP-sugars
-
-
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Co2+
Mg2+
Mn2+
Zn2+
additional information
Co2+
-
divalent metal required. Mn2+ (3 mM) is 63% as effective as Mg2+ (3 mM)
Mg2+
absolute requirement for divalent cation, maximal activity in presence of Mg2+ or Mn2+. Maximal activity in presence of 2 mM Mg2+
Mg2+
-
divalent metal required. Order of decreasing effectiveness: Mg2+ > Mn2+ > Co2+. Optimal concentration of Mg2+ is 5 mM
Mg2+
the enzyme requires bivalent ions, Mg2+ or Mn2+, for its activity
Mn2+
-
divalent metal required. Mn2+ (3 mM) is 87% as effective as Mg2+ (3 mM)
Mn2+
the enzyme requires bivalent ions, Mg2+ or Mn2+, for its activity
additional information
the enzyme requires bivalent cations for activity. HvUSP accepts other bivalent cation such as Ca2+, Mn2+, and Zn2+, but prefers Mg2+
additional information
the enzyme requires bivalent cations for activity. HvUSP accepts other bivalent cation such as Co2+, Ni2+, Mn2+ and Zn2+, but prefers Mg2+
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
(3R)-1-(4-chlorobenzoyl)-N-(3,4-dihydro-2H-1,5-benzodioxepin-7-yl)piperidine-3-carboxamide
compound (3R)-1-(4-chlorobenzoyl)-N-(3,4-dihydro-2H-1,5-benzodioxepin-7-yl)piperidine-3-carboxamide preferentially binds to one site located in the proximity of the active center of the enzyme
2-(furan-2-yl)-N'-(indol-3-ylidenemethyl)quinoline-4-carbohydrazide
-
2-[(3As,7aR)-1,3-dioxo-3a,4,7,7a-tetrahydroisoindol-2-yl]-N-[4-(propan-2-ylsulfamoyl)phenyl]acetamide
-
2-[3-(trifluoromethyl)anilino]-1,3-thiazol-4-one
compound 2-[3-(trifluoromethyl)anilino]-1,3-thiazol-4-on preferentially binds to one site located in the proximity of the active center of the enzyme
ethyl 2,4-dimethyl-5-[(2R)-2-[4-(2-phenoxyethyl)piperazin-1-yl]propanoyl]-1H-pyrrole-3-carboxylate
-
hydrogen peroxide
inactivation, reactivation by reduction using cysteine or thioredoxin
methyl (2R)-2-[[2-(4-fluorophenyl)sulfanylacetyl]amino]-3-hydroxypropanoate
-
nitric oxide
inactivation, reactivation by reduction using cysteine or thioredoxin
additional information
identification of structurally diverse scaffolds for the development of USP inhibitors by fragment library screening, selection of small molecule inhibitors with allosteric regulation sites, that inhibit both Leishmania major USP and UGP. Inhibitor binding kinetics, computational docking study, overview
-
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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DISEASE
TITLE OF PUBLICATION
LINK TO PUBMED
Dehydration
The Promoter of AtUSP Is Co-regulated by Phytohormones and Abiotic Stresses in Arabidopsis thaliana.
Infections
Functional characterization of the Arabidopsis universal stress protein AtUSP with an antifungal activity.
utp-monosaccharide-1-phosphate uridylyltransferase deficiency
UDP-sugar pyrophosphorylase - a new old mechanism for sugar activation.
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
8.14
dTTP
-
pH not specified in the publication, temperature not specified in the publication
3.74
dUTP
-
pH not specified in the publication, temperature not specified in the publication
0.373
UDP-beta-L-arabinopyranose
reverse reaction, pH 7.8, 25°C
0.2765
alpha-D-galactose 1-phosphate
mutant V330W/F383R, pH and temperature not specified in the publication