EC Number   |
Protein Variants |
Reference |
|---|
   2.7.7.64 | F383R |
site-directed mutagenesis, the mutant shows 88% reduction of catalytic efficiency compared to the wild-type enzyme enzyme with substrate alpha-D-galactose 1-phosphate |
761959 |
| 2.7.7.64 | more |
functional UDP-sugar diphosphorylase from Arabidopsis thaliana, constitutively expressed in Pichia pastoris and secreted into the extracellular medium, is used for synthesis of UDP-alpha-D-glucuronate, purification of the UDP-sugar from medium by anion exchange chromatography. Purification and assay methods optimization, overview. The fermentation of Pichia pastoris strain SMD1168H-C-AtUSP is performed at 30°C and pH 7.0 throughout the procedure, 20% glucose is supplemented at a rate of 9 ml/h, and DO is maintained at over 20%. After 60 h, the cell wet weight stabilizes (a wet weight of 276.7 g/l is obtained at 96 h). The crude enzyme activity increases up to 84 h, then it is stabilized, with activity 1601 U/ml. Thus, USP activity increases in 5-l fermenter compared with that in shaken flasks. The optimal temperature is around 35°C, lower than the optimal temperature of enzymatic reaction by purified AtUSP (45°C), while the optimal pH value and metal ion conditions are close to those of the purified enzyme system |
760379 |
| 2.7.7.64 | more |
UDP-sugar pyrophosphorylase from Hordeum vulgare, with favorable biochemical properties like broad pH and temperature tolerances as well as a broad substrate spectrum and high synthesis stability, is used for efficient synthesis of nucleotide sugars under conditions optimized by high-through-put multiplexed capillary electrophoresis analysis in combination with a galactokinase UDP-alpha-D-galactose (UDP-Gal). Assay optimization, overview |
761567 |
| 2.7.7.64 | V199W |
site-directed mutagenesis, the mutant shows 78% reduction of catalytic efficiency compared to the wild-type enzyme with substrate alpha-D-galactose 1-phosphate |
761959 |
| 2.7.7.64 | V330W |
site-directed mutagenesis, the mutant shows 38% reduction of catalytic efficiency compared to the wild-type enzyme enzyme with substrate alpha-D-galactose 1-phosphate |
761959 |
| 2.7.7.64 | V330W/F383R |
site-directed mutagenesis, the mutant shows 77% reduction of catalytic efficiency compared to the wild-type enzyme enzyme with substrate alpha-D-galactose 1-phosphate |
761959 |
