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Literature summary for 2.7.7.64 extracted from

  • Kotake, T.; Yamaguchi, D.; Ohzono, H.; Hojo, S.; Kaneko, S.; Ishida, H.K.; Tsumuraya, Y.
    UDP-sugar pyrophosphorylase with broad substrate specificity toward various monosaccharide 1-phosphates from pea sprouts (2004), J. Biol. Chem., 279, 45728-45736.
    View publication on PubMed
Search for more literature for 2.7.7.64

Cloned(Commentary)

Cloned (Comment) Organism
-
 Pisum sativum
His-tagged thioredoxin fusion protein expressed in Escherichia coli BL21gold strain Pisum sativum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.048
-
 UTP pH 7.0, 35°C Pisum sativum
0.048
-
 UTP D-glucose 1-phosphate, pH 7.0, 35°C Pisum sativum
0.25
-
 diphosphate pH 7.0, 35°C Pisum sativum
0.34
-
 UDP-glucose pH 7.0, 35°C Pisum sativum
0.34
-
 D-glucose 1-phosphate pH 7.0, 35°C Pisum sativum
0.34
-
 UDP-D-glucose pH 7.0, 35°C Pisum sativum
0.48
-
 D-glucuronic acid 1-phosphate pH 7.0, 35°C Pisum sativum
0.58
-
 D-galactose 1-phosphate pH 7.0, 35°C Pisum sativum
0.96
-
 L-arabinose 1-phosphate pH 7.0, 35°C Pisum sativum
0.96
-
 arabinose 1-phosphate pH 7.0, 35°C Pisum sativum
1.98
-
 D-xylose 1-phosphate pH 7.0, 35°C Pisum sativum

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Pisum sativum
Mg2+ absolute requirement for divalent cation, maximal activity in presence of Mg2+ or Mn2+. Maximal activity in presence of 2 mM Mg2+ Pisum sativum
Mn2+ 75% activity in comarison with Mg2+ Pisum sativum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
66040
-
 x * 66040, calculated from sequence Pisum sativum
67000
-
 x * 67000, SDS-PAGE Pisum sativum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UTP + a monosaccharide 1-phosphate Pisum sativum the enzyme catalyzes the formation of various UDP-sugars at the end of salvage pathways diphosphate + UDP-monosaccharide
-
 ?

Organism

Organism UniProt Comment Textmining
Pisum sativum Q5W915
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate fractionation, ion exchange chromatography (DEAE), hydrophobic interaction chromatography, gel filtration and ion exchange chromatography (DEAE), recombinant protein: immobilized metal ion affinity chromatography, splitting off the fused thioredoxin and His-tag, ion exchange chromatography (DEAE) Pisum sativum
recombinant Pisum sativum

Source Tissue

Source Tissue Comment Organism Textmining
sprout
-
 Pisum sativum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
30
-
 UDP-L-arabinose pyrophosphorylase, pH 7.0, 35°C Pisum sativum
63.9
-
-
 Pisum sativum
63.9
-
 UDP-D-glucose pyrophosphorylase, pH 7.0, 35°C Pisum sativum
95
-
 recombinant UDP-glucose pyrophosphorylase, pH 7.0, 35°C Pisum sativum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diphosphate + UDP-D-glucose
-
 Pisum sativum UTP + D-glucose 1-phosphate
-
 r
UTP + a monosaccharide 1-phosphate the enzyme catalyzes the formation of various UDP-sugars at the end of salvage pathways Pisum sativum diphosphate + UDP-monosaccharide
-
 ?
UTP + arabinose 1-phosphate 70.5% of enzyme activity with glucose 1-phosphate Pisum sativum diphosphate + UDP-arabinose
-
 r
UTP + D-galactose 1-phosphate
-
 Pisum sativum diphosphate + UDP-galactose
-
 ?
UTP + D-galactose 1-phosphate 116.2% of enzyme activity with glucose 1-phosphate Pisum sativum diphosphate + UDP-galactose
-
 r
UTP + D-glucose 1-phosphate
-
 Pisum sativum diphosphate + UDP-glucose
-
 r
UTP + D-glucose 1-phosphate UTP specific Pisum sativum diphosphate + UDP-glucose
-
 r
UTP + D-glucuronic acid 1-phosphate 71.3% of enzyme activity with glucose 1-phosphate Pisum sativum diphosphate + UDP-D-glucuronic acid
-
 r
UTP + D-glucuronic acid 1-phosphate
-
 Pisum sativum diphosphate + UDP-glucuronic acid
-
 ?
UTP + D-xylose 1-phosphate
-
 Pisum sativum diphosphate + UDP-alpha-D-xylopyranose
-
 ?
UTP + D-xylose 1-phosphate 35.9% of enzyme activity with glucose 1-phosphate Pisum sativum diphosphate + UDP-xylose
-
 r
UTP + L-arabinose 1-phosphate
-
 Pisum sativum diphosphate + UDP-beta-L-arabinopyranose
-
 ?
UTP + N-acetyl-D-glucosamine 1-phosphate 4.3% of enzyme activity with glucose 1-phosphate Pisum sativum diphosphate + UDP-N-acetylglucosamine
-
 r

Subunits

Subunits Comment Organism
? x * 67000, SDS-PAGE Pisum sativum
? x * 66040, calculated from sequence Pisum sativum

Synonyms

Synonyms Comment Organism
PsUSP
-
 Pisum sativum
UDP-sugar pyrophosphorylase
-
 Pisum sativum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Pisum sativum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 loss of activity of more than 99% at 55°C Pisum sativum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7.5
-
 Pisum sativum
7
-
 pH 6.5-7.5, formation of UDP-glucose Pisum sativum

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.5 8 80% loss of activity below pH 5.0 Pisum sativum

pI Value

Organism Comment pI Value Maximum pI Value
Pisum sativum calculated
-
 5.82