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Literature summary for 2.7.7.64 extracted from
- UDP-sugar pyrophosphorylase with broad substrate specificity toward various monosaccharide 1-phosphates from pea sprouts (2004), J. Biol. Chem., 279, 45728-45736.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Lathyrus oleraceus |
| His-tagged thioredoxin fusion protein expressed in Escherichia coli BL21gold strain | Lathyrus oleraceus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.048 | - |
UTP | pH 7.0, 35°C | Lathyrus oleraceus |  |
| 0.048 | - |
UTP | D-glucose 1-phosphate, pH 7.0, 35°C | Lathyrus oleraceus | |
| 0.25 | - |
diphosphate | pH 7.0, 35°C | Lathyrus oleraceus | |
| 0.34 | - |
D-glucose 1-phosphate | pH 7.0, 35°C | Lathyrus oleraceus | |
| 0.34 | - |
UDP-glucose | pH 7.0, 35°C | Lathyrus oleraceus | |
| 0.34 | - |
UDP-D-glucose | pH 7.0, 35°C | Lathyrus oleraceus | |
| 0.48 | - |
D-glucuronic acid 1-phosphate | pH 7.0, 35°C | Lathyrus oleraceus | |
| 0.58 | - |
D-galactose 1-phosphate | pH 7.0, 35°C | Lathyrus oleraceus | |
| 0.96 | - |
L-arabinose 1-phosphate | pH 7.0, 35°C | Lathyrus oleraceus | |
| 0.96 | - |
arabinose 1-phosphate | pH 7.0, 35°C | Lathyrus oleraceus | |
| 1.98 | - |
D-xylose 1-phosphate | pH 7.0, 35°C | Lathyrus oleraceus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | absolute requirement for divalent cation, maximal activity in presence of Mg2+ or Mn2+. Maximal activity in presence of 2 mM Mg2+ | Lathyrus oleraceus | |
| Mg2+ | - |
Lathyrus oleraceus | |
| Mn2+ | 75% activity in comarison with Mg2+ | Lathyrus oleraceus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 66040 | - |
x * 66040, calculated from sequence | Lathyrus oleraceus |
| 67000 | - |
x * 67000, SDS-PAGE | Lathyrus oleraceus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UTP + a monosaccharide 1-phosphate | Lathyrus oleraceus | the enzyme catalyzes the formation of various UDP-sugars at the end of salvage pathways | diphosphate + UDP-monosaccharide | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lathyrus oleraceus | Q5W915 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate fractionation, ion exchange chromatography (DEAE), hydrophobic interaction chromatography, gel filtration and ion exchange chromatography (DEAE), recombinant protein: immobilized metal ion affinity chromatography, splitting off the fused thioredoxin and His-tag, ion exchange chromatography (DEAE) | Lathyrus oleraceus |
| recombinant | Lathyrus oleraceus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| sprout | - |
Lathyrus oleraceus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 30 | - |
UDP-L-arabinose pyrophosphorylase, pH 7.0, 35°C | Lathyrus oleraceus |
| 63.9 | - |
- |
Lathyrus oleraceus |
| 63.9 | - |
UDP-D-glucose pyrophosphorylase, pH 7.0, 35°C | Lathyrus oleraceus |
| 95 | - |
recombinant UDP-glucose pyrophosphorylase, pH 7.0, 35°C | Lathyrus oleraceus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diphosphate + UDP-D-glucose | - |
Lathyrus oleraceus | UTP + D-glucose 1-phosphate | - |
r | |
| UTP + a monosaccharide 1-phosphate | the enzyme catalyzes the formation of various UDP-sugars at the end of salvage pathways | Lathyrus oleraceus | diphosphate + UDP-monosaccharide | - |
? | |
| UTP + arabinose 1-phosphate | 70.5% of enzyme activity with glucose 1-phosphate | Lathyrus oleraceus | diphosphate + UDP-arabinose | - |
r | |
| UTP + D-galactose 1-phosphate | - |
Lathyrus oleraceus | diphosphate + UDP-galactose | - |
? | |
| UTP + D-galactose 1-phosphate | 116.2% of enzyme activity with glucose 1-phosphate | Lathyrus oleraceus | diphosphate + UDP-galactose | - |
r | |
| UTP + D-glucose 1-phosphate | - |
Lathyrus oleraceus | diphosphate + UDP-glucose | - |
r | |
| UTP + D-glucose 1-phosphate | UTP specific | Lathyrus oleraceus | diphosphate + UDP-glucose | - |
r | |
| UTP + D-glucuronic acid 1-phosphate | 71.3% of enzyme activity with glucose 1-phosphate | Lathyrus oleraceus | diphosphate + UDP-D-glucuronic acid | - |
r | |
| UTP + D-glucuronic acid 1-phosphate | - |
Lathyrus oleraceus | diphosphate + UDP-glucuronic acid | - |
? | |
| UTP + D-xylose 1-phosphate | - |
Lathyrus oleraceus | diphosphate + UDP-alpha-D-xylopyranose | - |
? | |
| UTP + D-xylose 1-phosphate | 35.9% of enzyme activity with glucose 1-phosphate | Lathyrus oleraceus | diphosphate + UDP-xylose | - |
r | |
| UTP + L-arabinose 1-phosphate | - |
Lathyrus oleraceus | diphosphate + UDP-beta-L-arabinopyranose | - |
? | |
| UTP + N-acetyl-D-glucosamine 1-phosphate | 4.3% of enzyme activity with glucose 1-phosphate | Lathyrus oleraceus | diphosphate + UDP-N-acetylglucosamine | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 66040, calculated from sequence | Lathyrus oleraceus |
| ? | x * 67000, SDS-PAGE | Lathyrus oleraceus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PsUSP | - |
Lathyrus oleraceus |
| UDP-sugar pyrophosphorylase | - |
Lathyrus oleraceus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Lathyrus oleraceus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
loss of activity of more than 99% at 55°C | Lathyrus oleraceus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 7.5 | - |
Lathyrus oleraceus |
| 7 | - |
pH 6.5-7.5, formation of UDP-glucose | Lathyrus oleraceus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 8 | 80% loss of activity below pH 5.0 | Lathyrus oleraceus |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Lathyrus oleraceus | calculated | - |
5.82 |
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