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Literature summary for 2.7.7.64 extracted from

  • Kim, B.; Ahn, J.
    Characterization of uridine diphosphate-sugar pyrophosphorylase from Populus deltoids (2013), J. Korean Soc. Appl. Biol. Chem., 56, 525-531.
No PubMed abstract available
Search for more literature for 2.7.7.64

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Populus deltoides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.49
-
 D-glucose 1-phosphate pH 8.0, 37°C Populus deltoides
1.98
-
 UTP pH 8.0, 37°C Populus deltoides

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
77500
-
-
 Populus deltoides

Organism

Organism UniProt Comment Textmining
Populus deltoides
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-galactose 1-phosphate + UTP 30% of the activity with D-glucose 1-phosphate Populus deltoides UDP-D-galactose + diphosphate
-
 ?
D-glucose 1-phosphate + UTP
-
 Populus deltoides UDP-D-glucose + diphosphate
-
 ?
additional information no substrate: D-mannose 1-phosphate Populus deltoides ?
-
 ?

Subunits

Subunits Comment Organism
? x * 77500, SDS-PAGE, recombinant protein with GHST-tag Populus deltoides

Synonyms

Synonyms Comment Organism
USPase
-
 Populus deltoides

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.003
-
 UTP pH 8.0, 37°C Populus deltoides
0.008
-
 D-glucose 1-phosphate pH 8.0, 37°C Populus deltoides

General Information

General Information Comment Organism
physiological function enzyme is able to complement an Escherichia coli GalU mutant Populus deltoides

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0015
-
 UTP pH 8.0, 37°C Populus deltoides
0.0054
-
 D-glucose 1-phosphate pH 8.0, 37°C Populus deltoides