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Literature summary for 2.7.7.64 extracted from
- Biosynthesis of nucleotide sugars by a promiscuous UDP-sugar pyrophosphorylase from Arabidopsis thaliana (AtUSP) (2013), Bioorg. Med. Chem. Lett., 23, 3764-3768.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | use of UDP-sugar pyrophosphorylase (USP) from Arabidopsis thaliana with a galactokinase from Streptococcus pneumoniae TIGR4 (SpGalK) and an inorganic diphosphatase (PPase) to effectively synthesize UDP-sugars | Arabidopsis thaliana |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.88 | - |
UTP | pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana |  |
| 3.74 | - |
dUTP | pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
| 8.14 | - |
dTTP | pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-azido-D-galactose 1-phosphate + UTP | - |
Arabidopsis thaliana | UDP-4-azido-D-galactose + diphosphate | 43% conversion rate | ? | |
| 6-deoxy-D-galactose 1-phosphate + UTP | - |
Arabidopsis thaliana | UDP-6-deoxy-D-galactose + diphosphate | 29% conversion rate | ? | |
| D-galactose 1-phosphate + dTTP | - |
Arabidopsis thaliana | dTDP-D-galactose + diphosphate | - |
? | |
| D-galactose 1-phosphate + dUTP | - |
Arabidopsis thaliana | dUDP-D-galactose + diphosphate | - |
? | |
| D-galactose 1-phosphate + UTP | - |
Arabidopsis thaliana | UDP-D-galactose + diphosphate | 95% conversion rate | ? | |
| D-glucose 1-phosphate + UTP | - |
Arabidopsis thaliana | UDP-D-glucose + diphosphate | 28% conversion rate | ? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| USP | - |
Arabidopsis thaliana |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 27 | - |
dTTP | pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
| 219 | - |
dUTP | pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
| 348 | - |
UTP | pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.3 | - |
dTTP | pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
| 59 | - |
dUTP | pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
| 121 | - |
UTP | pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
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