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Literature summary for 2.7.7.64 extracted from

  • Ebrecht, A.C.; Asencion Diez, M.D.; Piattoni, C.V.; Guerrero, S.A.; Iglesias, A.A.
    The UDP-glucose pyrophosphorylase from Giardia lamblia is redox regulated and exhibits promiscuity to use galactose-1-phosphate (2015), Biochim. Biophys. Acta, 1850, 88-96.
    View publication on PubMed
Search for more literature for 2.7.7.64

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Giardia duodenalis

Inhibitors

Inhibitors Comment Organism Structure
hydrogen peroxide inactivation, reactivation by reduction using cysteine or thioredoxin Giardia duodenalis
nitric oxide inactivation, reactivation by reduction using cysteine or thioredoxin Giardia duodenalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.08
-
 UTP cosubstrate D-galactose 1-phosphate, S0.5 value, pH 8.0, 37°C Giardia duodenalis
0.09
-
 D-galactose 1-phosphate cosubstrate UTP, S0.5 value, pH 8.0, 37°C Giardia duodenalis
0.13
-
 D-glucose 1-phosphate cosubstrate UTP, S0.5 value, pH 8.0, 37°C Giardia duodenalis
0.14
-
 UTP cosubstrate D-glucose 1-phosphate, S0.5 value, pH 8.0, 37°C Giardia duodenalis
0.36
-
 D-glucose 1-phosphate cosubstrate TTP, S0.5 value, pH 8.0, 37°C Giardia duodenalis
0.9
-
 TTP cosubstrate D-glucose 1-phosphate, S0.5 value, pH 8.0, 37°C Giardia duodenalis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ strict dependance on Mg2+ Giardia duodenalis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49300
-
-
 Giardia duodenalis
50000
-
 gel filtration Giardia duodenalis

Organism

Organism UniProt Comment Textmining
Giardia duodenalis A8BJJ7
-
-
Giardia duodenalis ATCC 50803 A8BJJ7
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-galactose 1-phosphate + TTP
-
 Giardia duodenalis TDP-D-galactose + diphosphate
-
 ?
D-galactose 1-phosphate + TTP
-
 Giardia duodenalis ATCC 50803 TDP-D-galactose + diphosphate
-
 ?
D-galactose 1-phosphate + UTP
-
 Giardia duodenalis UDP-D-galactose + diphosphate
-
 ?
D-galactose 1-phosphate + UTP
-
 Giardia duodenalis ATCC 50803 UDP-D-galactose + diphosphate
-
 ?
D-glucose 1-phosphate + UTP preferred substrates Giardia duodenalis UDP-D-glucose + diphosphate
-
 ?
D-glucose 1-phosphate + UTP preferred substrates Giardia duodenalis ATCC 50803 UDP-D-glucose + diphosphate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 49300, calculated, 1 * 50000, SDS-PAGE Giardia duodenalis

Synonyms

Synonyms Comment Organism
GL50803_29307
-
 Giardia duodenalis