Literature summary for 2.7.7.64 extracted from

Ebrecht, A.C.; Asencion Diez, M.D.; Piattoni, C.V.; Guerrero, S.A.; Iglesias, A.A.
The UDP-glucose pyrophosphorylase from Giardia lamblia is redox regulated and exhibits promiscuity to use galactose-1-phosphate (2015), Biochim. Biophys. Acta, 1850, 88-96.

Search for more literature for 2.7.7.64

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Giardia intestinalis

Inhibitors

Inhibitors	Comment	Organism	Structure
hydrogen peroxide	inactivation, reactivation by reduction using cysteine or thioredoxin	Giardia intestinalis
nitric oxide	inactivation, reactivation by reduction using cysteine or thioredoxin	Giardia intestinalis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.08	-	UTP	cosubstrate D-galactose 1-phosphate, S0.5 value, pH 8.0, 37°C	Giardia intestinalis
0.09	-	D-galactose 1-phosphate	cosubstrate UTP, S0.5 value, pH 8.0, 37°C	Giardia intestinalis
0.13	-	D-glucose 1-phosphate	cosubstrate UTP, S0.5 value, pH 8.0, 37°C	Giardia intestinalis
0.14	-	UTP	cosubstrate D-glucose 1-phosphate, S0.5 value, pH 8.0, 37°C	Giardia intestinalis
0.36	-	D-glucose 1-phosphate	cosubstrate TTP, S0.5 value, pH 8.0, 37°C	Giardia intestinalis
0.9	-	TTP	cosubstrate D-glucose 1-phosphate, S0.5 value, pH 8.0, 37°C	Giardia intestinalis

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	strict dependance on Mg2+	Giardia intestinalis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
49300	-	-	Giardia intestinalis
50000	-	gel filtration	Giardia intestinalis

Organism

Organism	UniProt	Comment	Textmining
Giardia intestinalis	A8BJJ7	-	-
Giardia intestinalis ATCC 50803	A8BJJ7	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-galactose 1-phosphate + TTP	-	Giardia intestinalis	TDP-D-galactose + diphosphate	-	?
D-galactose 1-phosphate + UTP	-	Giardia intestinalis	UDP-D-galactose + diphosphate	-	?
D-glucose 1-phosphate + UTP	preferred substrates	Giardia intestinalis	UDP-D-glucose + diphosphate	-	?
D-glucose 1-phosphate + UTP	preferred substrates	Giardia intestinalis ATCC 50803	UDP-D-glucose + diphosphate	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 49300, calculated, 1 * 50000, SDS-PAGE	Giardia intestinalis

Synonyms

Synonyms	Comment	Organism
GL50803_29307	-	Giardia intestinalis

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)