Information on EC 2.7.1.35 - pyridoxal kinase

additional information

experimental resurrection of the last common ancestor of the hydroxymethyl pyrimidine kinase group based on comparison of hydroxymethyl pyrimidine and pyridoxal kinases. Probably the last common ancestor was not able to use pyridoxal under physiological conditions. The pyridoxal kinase activity present in the current bifunctional enzymes must have appeared in a convergent event independently of the pyridoxal kinase activity of pdxY and pdxK genes. Substrate pyridoxal is 8-times less preferred than the phosphorylation of hydroxymethyl pyrimidine by the last ancestor

738234

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

ATP + 4'-deoxypyridoxine

ADP + 4'-deoxypyridoxine 5'-phosphate

ATP + 4'-O-methylpyridoxine

ADP + 4'-O-methylpyridoxine 5'-phosphate

ATP + 4-amino-5-hydroxymethyl-2-methylpyrimidine

ADP + 4-amino-5-phosphomethyl-2-methylpyrimidine

ATP + 4-deoxypyridoxine

ADP + 4-deoxypyridoxine 5'-phosphate

ATP + 5-deoxypyridoxal

ADP + ?

ATP + ginkgotoxin

ginkgotoxin is an effective pseudosubstrate

ATP + ginkgotoxin

ADP + ginkgotoxin 5'-phosphate

ATP + omega-methylpyridoxal

ADP + omega-methylpyridoxal 5'-phosphate

ATP + pyridoxal

ADP + pyridoxal 5'-phosphate

78 entries

ATP + pyridoxamine

ADP + pyridoxamine 5'-phosphate

14 entries

ATP + pyridoxine

ADP + pyridoxine 5'-phosphate

25 entries

methyl (1S)-1-[3-hydroxy-5-(hydroxymethyl)-2-methylpyridin-4-yl]-2,3,4,9-tetrahydro-1H-beta-carboline-3-carboxylate + ATP

methyl (1S)-1-[3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl]-2,3,4,9-tetrahydro-1H-beta-carboline-3-carboxylate + ADP

very probably: methyl (1S)-1-[3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl]-2,3,4,9-tetrahydro-1H-beta-carboline-3-carboxylate is an inhibitor of Plasmodium falciparum ornithine decarboxylase

methyl N-[[3-hydroxy-5-(hydroxymethyl)-2-methylpyridin-4-yl]methyl]histidinate + ATP

methyl N-([3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl]methyl)histidinate + ADP

methyl N-([3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl]methyl)histidinate is an inhibitor of Plasmodium falciparum ornithine decarboxylase: IC50 = 0.058 mM

methyl N-[[3-hydroxy-5-(hydroxymethyl)-2-methylpyridin-4-yl]methyl]tryptophanate + ATP

methyl N-([3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl]methyl)tryptophanate + ADP

methyl N-([3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl]methyl)tryptophanate is an inhibitor of Plasmodium falciparum ornithine decarboxylase: IC50 = 0.064 mM

pyridoxal + ATP

pyridoxal 5'-phosphate

pyridoxal + ATP

pyridoxal 5'-phosphate + ADP

702038

pyridoxamine + ATP

pyridoxamine 5'-phosphate

pyridoxine + ATP

pyridoxine 5'-phosphate

additional information

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

ATP + pyridoxal

ADP + pyridoxal 5'-phosphate

22 entries

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

ATP

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

Ca2+

Co2+

Cu2+

Fe2+

K+

Li+

poor activator which seems to modify the enzymatic mechanism from a random to an ordered sequential pattern with ATP bound before pyridoxal. Km: 37 mM

Mg2+

Mn2+

Na+

Ni2+

Rb+

Km: 5.3 mM. Monovalent cation required, activation in the order of decreasing efficiency: K+, Rb+, NH4+

Zn2+

14 entries

additional information

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

(2-Diethylaminoethyl)hydrazine

0.005 mM, 20% inhibition

(R)-roscovitine

1,1-Bis(2-diethylaminoethyl)hydrazine

0.005 mM, 51% inhibition

1,4-diaminobutane

641406

1,4-Diaminopiperazine

0.005 mM, 59% inhibition

1,6-diaminohexane

1,8-diaminooctane

1-(2-Hydrazinopropyl)piperidine

0.005 mM, 31% inhibition

1-(3-Dimethylamino-2-methylpropyl)hydrazine

0.005 mM, 39% inhibition

1-Aminopiperidine

0.005 mM, 58% inhibition

1-Cyclohexyl-1-methylhydrazine

0.005 mM, 29% inhibition

2,2'-hydrazinediethanol

0.005 mM, 47% inhibition

2,6-Dimethyl-3,4-bis(hydroxymethyl)pyridine

2-Methyl-3-amino-4,5,6-tris(hydroxymethyl)pyridine

2-Methyl-3-amino-4,5-bis(hydroxymethyl)pyridine

2-Methyl-3-hydroxy-5,6-bis(hydroxymethyl)pyridine

2-Methyl-4,5-bis(hydroxymethyl)pyridine

2-[1-(2-Diethylaminoethyl)hydrazine]ethanol

0.005 mM, 44% inhibition

3,4-dihydroxyphenylalanine

641414

3-hydroxyanthranilic acid

IC50: 0.12 mM

3-hydroxykynurenine

IC50: 0.1 mM

4'-deoxypyridoxine

4'-O-methylpyridoxine

i.e. ginkgotoxin. Treatment leads to temporarily reduced pyridoxal phosphate formation in vitro and possibly in vivo

4-Aminomorpholine

0.005 mM, 52% inhibition

4-deoxypyridoxine

5-dimethylaminonaphthalene-1-sulfonyl-4-aminobutyrate

competitive with respect to pyridoxal

5-hydroxytryptamine

0.5 mM, 11% inhibition of activity with pyridoxine, 81% inhibition of activity with pyridoxal

alpha-Methylphenethylhydrazine

alpha-Methylphenethylhydrazone

aminooxyacetic acid

0.002 mM, 50% inhibition

AMP

azine

Ca2+

low substrate inhibition

cadaverine

Caffeine

21% inhibition at 0.1 mM

Chloroquine

Plasmodium vivax

A5K1Z8;

about 40% inhibition at 1 mM

Cr2+

cycloserine

0.1 mM, inhibits activity with pyridoxal, but not with pyridoxamine as substrate, 42% inhibition

D-penicillamine

0.1 mM, inhibits activity with pyridoxal, but not with pyridoxamine as substrate, 20% inhibition

dopamine

enprofylline

33% inhibition at 0.1 mM

gamma-aminobutyric acid

ginkgotoxin

histamine

hydrazine

hydroxylamine

isoniazid

0.1 mM, inhibits activity with pyridoxal, but not with pyridoxamine as substrate, 81% inhibition

K+

at high concentrations

L-canaline

0.01 mM, 50% inhibition

lamotrigine

45% inhibition at 0.1 mM

levodopa

0.1 mM, inhibits activity with pyridoxal, but not with pyridoxamine as substrate, 16% inhibition

Li+

at high concentrations

Mg2+

Mn2+

excess free divalent cations inhibit the enzyme

muzolimine

0.1 mM, inhibits activity with pyridoxal, but not with pyridoxamine as substrate, 27% inhibition

N,N'-Bis(pyridoxyl)hydrazine

0.0005 mM, 50% inhibition

N-Dansyl-1,8-diaminooctane

Na+

NADH

0.5 mM, 14% inhibition of activity with pyridoxine, 11% inhibition of activity with pyridoxal

norepinephrine

Octylhydrazine

0.005 mM, 53% inhibition

Oxime

picolinate

0.5 mM, 95% inhibition of activity with pyridoxine, 97% inhibition of activity with pyridoxal

primaquine

progabide

0.1 mM, inhibits using either pyridoxamine or pyridoxal as substrate

putrescine

pyridoxal

pyridoxal 5'-phosphate

737767

pyridoxal semicarbazone

0.00005 mM, 50% inhibition

pyridoxal-gamma-aminobutyrate

pyridoxaloxime

pyridoxamine

pyridoxine

substrate inhibition above 0.2 mM

quinolinate

0.5 mM, 82% inhibition of activity with pyridoxine, 82% inhibition of activity with pyridoxal

quinolinic acid

IC50: 0.42 mM

roscovitine

rugulactone

selectively modifies the enzyme not at the active site cysteine, but on a remote cysteine residue

Semicarbazine

0.0005 mM, 50% inhibition

serotonin

spermidine

weak

spermine

weak

Theobromine

22% inhibition at 0.1 mM

theophylline

Thiamphenicol

0.1 mM, inhibits activity with pyridoxal, but not with pyridoxamine as substrate, 31% inhibition

tryptamine

tyramine

xanthurenate

0.5 mM, 19% inhibition of activity with pyridoxine, 18% inhibition of activity with pyridoxal

xanthurenic acid

IC50: 0.36 mM

Zn2+

ZnATP2-

weak substrate inhibition

737809

additional information

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

NAD+

0.5 mM, activation to 125%, activity with pyridoxine

NH4+

phosphate

optimal concentration is 80-100 mM

641415

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

0.0216

4'-deoxypyridoxine

in 70 mM potassium phosphate buffer, pH 6.2; pH 6.2, 37°C

0.00495

4'-O-methylpyridoxine

pH 6.2, 37°C

1.85 - 2.03

4-Amino-5-hydroxymethyl-2-methylpyrimidine

0.0091 - 0.5

ATP

12 entries

0.00495 - 0.0466

ginkgotoxin

0.17 - 0.42

MgATP

0.101 - 0.6

MgATP2-

0.194

MnATP2-

pH 5.8, 37°C

0.00122 - 0.49

pyridoxal

32 entries

0.006 - 0.126

pyridoxamine

7 entries

0.00172 - 2.07

pyridoxine

15 entries

0.06 - 0.07

ZnATP2-

additional information

effect of KCl on Km-values

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

1.535 - 2.94

4'-deoxypyridoxine

0.46

4'-O-methylpyridoxine

pH 6.2, 37°C

0.044 - 0.36

4-Amino-5-hydroxymethyl-2-methylpyrimidine

1.23 - 3.3

ATP

0.46 - 106

ginkgotoxin

0.015 - 131

pyridoxal

25 entries

0.03 - 127

pyridoxamine

6 entries

0.015 - 122

pyridoxine

9 entries

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

12.7 - 23.1

ATP

0.000228

ginkgotoxin

recombinant enzyme, apparent value, in HEPES/MgCl2 pH 7.4, 0.15 mM ZnCl2, at 37°C

0.0009 - 127

pyridoxal

0.000214

pyridoxamine

recombinant enzyme, apparent value, in HEPES/MgCl2 pH 7.4, 0.15 mM ZnCl2, at 37°C

0.000254

pyridoxine

recombinant enzyme, apparent value, in HEPES/MgCl2 pH 7.4, 0.15 mM ZnCl2, at 37°C

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

0.0574

4'-deoxypyridoxine

; pH 6.2, 37°C

0.000414

4'-O-methylpyridoxine

pH 6.2, 37°C

0.001

4-deoxypyridoxine

pH 6.0, 37°C

0.002

5-dimethylaminonaphthalene-1-sulfonyl-4-aminobutyrate

pH 8.0, 37°C

0.06

AMP

0.228

enprofylline

in 20 mM sodium BES buffer, pH 7.2, at 37°C

0.000414 - 0.003

ginkgotoxin

0.056

lamotrigine

in 20 mM sodium BES buffer, pH 7.2, at 37°C

1.54

Mg2+

recombinant enzyme, apparent value, in 70 mM potassium phosphate pH 7.0, 0.15 mM ZnCl2, at 37°C

0.0572

primaquine

at pH 7.0 and 30°C

738236

0.0001

pyridoxal oxime

pH 7, 25°C

641427

0.002

pyridoxaloxime

pH 6.0, 37°C

0.05

theophylline

in 20 mM sodium BES buffer, pH 7.2, at 37°C

0.0398

Zn2+

recombinant enzyme, apparent value, in 70 mM potassium phosphate pH 7.0, 0.15 mM ZnCl2, at 37°C

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

0.12

3-hydroxyanthranilic acid

Ovis aries;

IC50: 0.12 mM

0.1

3-hydroxykynurenine

Ovis aries;

IC50: 0.1 mM

0.42

quinolinic acid

Ovis aries;

IC50: 0.42 mM

0.36

xanthurenic acid

Ovis aries;

IC50: 0.36 mM

0.037

Zn2+

Homo sapiens;

at pH 6.5 and 37°C

737809

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

0.00135

Lactobacillus casei

0.0036

0.004

brain enzyme

0.0125

0.02

substrate: methyl N-[[3-hydroxy-5-(hydroxymethyl)-2-methylpyridin-4-yl]methyl]tryptophanate

0.03

0.038

substrate: methyl (1S)-1-[3-hydroxy-5-(hydroxymethyl)-2-methylpyridin-4-yl]-2,3,4,9-tetrahydro-1H-beta-carboline-3-carboxylate

0.05

0.052

purified kinase 2 enzyme, pH 6.1, 37°C

0.064

substrate: methyl N-[[3-hydroxy-5-(hydroxymethyl)-2-methylpyridin-4-yl]methyl]histidinate

0.07

crude enzyme, in 70 mM potassium phosphate (pH 5.5), 0.5 mM ZnCl2, at 37°C

0.0713

0.112

0.1447

0.52

1.27

reaction with pyridoxal

1.42

reaction with pyridoxine

1.8

crude enzyme, in 70 mM potassium phosphate (pH 5.5), 0.5 mM ZnCl2, at 37°C

2.105

2.47

2.5

purified enzyme, pH 6.1, 37°C

2.7

purified kinase 1 enzyme, pH 6.1, 37°C

additional information

Lactobacillus delbrueckii subsp. bulgaricus

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

4.5

Lactobacillus casei

5.2

5.5 - 6

5.7

5.8 - 6.3

5.8

Saccharomyces cerevisiae

6.4

6.5

6.8

Saccharomyces cerevisiae

8.5

8.8

enzyme from leukocytes

641407

9.1

enzyme from erythrocytes

641407

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

4.5 - 8.5

the enzyme is inactive below pH 4.5. Enzyme activity decreases slowly above pH 6.0, to approximately 35% at pH 8.5

4.7 - 5.3

Lactobacillus casei

pH 4.7: about 60% of maximal activity, pH 5.3: about 40% of maximal activity

5 - 5.7

pH 5.0: about 90% of maximal activity, pH 5.7: about 45% of maximal activity

5 - 8

pH 5.0: about 70% of maximal activity, pH 8.0: about 50% of maximal activity

6 - 7

Neurospora crassa

pH 6.0: about 50% of maximal activity, pH 7.0: about 50% of maximal activity

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

Zn2+ as activating anion

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

641417

5.14

kinase 1, calculated from the deduced amino acid sequence

5.75

calculated from the deduced amino acid sequence

6.05

kinase 2, calculated from the deduced amino acid sequence

6.3

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

641406, 641407, 641408, 641415, 641424, 641428, 673904

granule cell

hippocampal pyramidal layer

dentate granule cell. Pyridoxal kinase immunoreactivity is not increased after induction of long-term potentiation and not involved in increase in the efficiency of high frequency stimulus-induced potentiation of populations spike amplitude when compared with saline-, or Tat-protein-treated animals

692561

Meriones unguiculatus

from CA1 region, strong immunoreactivity

weak activity

Saccharomyces cerevisiae

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

chloroplast

Arabidopsis thaliana

splice variant SOS4.1 (but not SOS4.2) is localized in chloroplasts

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482);

5tqi, download, 3D-view

SCOPe (5tqi)

CATH (5tqi)

A0A0H3KDL9

Burkholderia multivorans (strain ATCC 17616 / 249);

Entamoeba histolytica;

Escherichia coli (strain K12);

9 entries

Homo sapiens;

Lactobacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1);

8 entries

Ovis aries;

5trw, download, 3D-view

SCOPe (5trw)

CATH (5trw)

Q141E8

Paraburkholderia xenovorans (strain LB400);

5b6a, download, 3D-view

SCOPe (5b6a)

CATH (5b6a)

Q9HT57

Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1);

Staphylococcus aureus (strain Mu50 / ATCC 700699);

3zs7, download, 3D-view

SCOPe (3zs7)

CATH (3zs7)

O15927

Trypanosoma brucei;

3pzs, download, 3D-view

SCOPe (3pzs)

CATH (3pzs)

Q7CIR8

Yersinia pestis;

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

33000

x * 33000, SDS-PAGE

33100

x * 33100, calculated

33900

2 * 33900, SDS-PAGE

34861

x * 34861, calculation from nucleotide sequence

35000

37000

39500

2 * 39500, SDS-PAGE

40000

57200

2 * 57200, calculated from the deduced amino acid sequence, 2 * 58000, SDS-PAGE, native mass by gel filtration

58000

2 * 57200, calculated from the deduced amino acid sequence, 2 * 58000, SDS-PAGE, native mass by gel filtration

60000

gel filtration, equilibrium sedimentation

65000

gel filtration

68000

gel filtration

73000

gel filtration

80000

110000

gel filtration

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

8 entries

dimer

8 entries

hexamer

trimer or hexamer, X-ray crystallography

homodimer

monomer

trimer

trimer or hexamer, X-ray crystallography

671140

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

hanging drop vapour diffusion method using 28% PEG 4000, 0.17 M sodium acetate trihydrate, and 0.1 M Tris-HCl (pH 8.5)

671140

with 28% (w/v) PEG 4000, 0.17 M sodium acetate trihydrate, 0.1 M Tris-HCl (pH 8.5) as the precipitant in the presence of 10 mM ADP and 10 mM MgCl

675407

hanging drop vapour diffusion method using 20 mM potassium phosphate, pH 7.5, 5 mM beta-mercaptoethanol, 0.2 mM EDTA, and 21% PEG 4K, 100 mM Tris-HCl, pH 8.5, 200 mM Na acetate, 40 mM MgSO4, and 10% glycerol

hanging drop vapour diffusion method using 0.1 mM Tris-HCl pH 8.0, 1.8 M NH4Ac, and 3% glycol

675693

hanging drop vapour diffusion method using 100 mM Tris-HCl pH 8.0 and 50% 2-methyl-2,4-pentanediol; unliganded enzyme and in complex with MgATP, diffraction to 2.0 and 2.2 A rsolution, respectively. both Structures show similar open conformations. Mg2+ and Na+ act in tandem to anchor the ATP at the active site, which itself acts as a sink to bind several molecules of 2-methyl-2,4-pentanediol

677029

in complex with ginkgotoxin and theophylline, hanging drop vapor diffusion method, using 48-50% (w/v) 2-methyl-1,3 propanediol as precipitant, at 22°C

crystallized in the orthorhombic form using the hanging-drop vapour-diffusion method with sodium citrate as the precipitant, crystals are transferred into a soaking liquid without citrate and two-heavy-atom derivatives are prepared

641420

crystals are grown in the presence of 100 mM potassium phosphate, pH 7.2, and 120 mM ammonium sulfate and crystals grown in the presence of 600 mM potassium phosphate, pH 7.2, and in the absence of ammonium sulfate. The crystals are quite stable to X-rays and diffract at 2.2 A resolution

641410

hanging drop vapor diffusion method in complex with adenosine 5’-(beta, gamma-methylenetriphosphate)-pyridoxamine, ADP-pyriodoxal 5’-phosphate, and ADP

662200

hanging drop vapor diffusion method, in complex with (R)-roscovitine and N6-methyl-(R)-roscovitine

P82197;

662402

A0A1C3KUB4;

737378

sitting drop vapor diffusion method, using 0.1 M Tris, pH 8.5, 1 M LiCl2, and 15% (w/v) PEG 6000

native protein and in complex with its substrates to 1.4?1.85 A resolution. The protein shows a typical ribokinase fold with a central large beta-sheet consisting of nine strands, flanked by three and five structurally conserved alpha-helices

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

5.5 - 11

25°C, 3 h, stable. Gradual irreversible inactivation below pH 5.5

641425

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

-20

stable at -20°C for approximately 21 days

pH 5.5-11, 3 h, stable

greatest stability is at below 40°C

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

3 M guanidine hydrochloride, enzyme loses more than 90% of the alpha-helix content

if instead of the phosphate buffer an acetate buffer is used, enzymatic activity is reduced to 74% and almost no activity is recorded when a citrate buffer is used

impure enzyme is stabilized by Zn2+

stable for 48 h when transduced into PC-12 cells

672438

the enzyme is digested by chymotrypsin to proteolytic fragments of 24000 Da and 16000 Da

641427

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

-18°C, stable for 20 weeks

641417

-20°C, recombinant enzyme, stable for 21 days

-80°C, 20 mM phosphate buffer, pH 7.3, stable for at least some weeks

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

10 entries

from hippocampal tissue

Meriones unguiculatus

662961

Hi-Load Superdex 200 gel filtration

675407

immobilized metal ion adsorption chromatography (Ni2+)

702038

Ni Sepharose affinity column chromatography

Ni-NTA agarose column chromatography

Ni-NTA agarose column chromatography and HiLoad 26/60 Superdex 200 gel filtration

675693

Ni-NTA column chromatography

737809

Ni-NTA column chromatography and Mono Q column chromatography

Ni-NTA Sepharose column chromatography

672438

Phenyl ToyoPearl 650S column chromatography and Hi-Load Superdex 200 column gel filtration

671140

pyridoxyl-EAH-Sepharose 4B column chromatography; recombinant enzyme

Q-Sepharose column chromatography and Superdex 75 gel filtration

recombinant enzyme

recombinant protein using His-tag

662007

recombinant protein using Strep-tag

TMAE column chromatography, Phenyl Sepharose column chromatography, and hydroxyapatite column chromatography

677029

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

;

Brassica napus

A4K866;, A4K867;

701442

; expressed in Escherichia coli strain BL21 (DE3)

expressed as His-tag fusion protein in Escherichia coli BL21(DE3)

662007

expressed as Strep-tag fusion protein in Escherichia coli BLR(DE3)

expressed in Escherichia coli

expressed in Escherichia coli BL21 (DE3) cells

672438

expressed in Escherichia coli BL21(DE3) cells

expressed in Escherichia coli HMS174(DE3)

expressed in Escherichia coli Rosetta (DE3) cells

expressed in Escherichia coli Rosetta (DE3)pLysS cells; expression in Escherichia coli

677029

expressed in Escherichia coli strain BL21 (DE3) plysS

675693

expression in Escherichia coli

His-tag, expressed in Escherichia coli Rosetta (lambdaDE3)

702038

kinase 1 and 2 expressed in Escherichia coli HMS174(DE3), kinase 2 additionally expressed as His-tag fusion protein

recombinant enzyme is overexpressed in Escherichia coli as a fusion protein with maltose binding protein

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

expression patterns show that the gene is involved in abiotic stress and phytohormone regulation; expression patterns show that the gene is involved in abiotic stress and phytohormone regulation

Brassica napus

A4K866;, A4K867;

701442

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

K229Q

D235A

active site residue

D235N

active site residue

C124A

the mutant shows about 2fold increased catalytic efficiency compared to the wild type enzyme

D225A

inactive

Pseudomonas aeruginosa PAO

C124A

the mutant shows about 2fold increased catalytic efficiency compared to the wild type enzyme

D225A

Pseudomonas aeruginosa PAO

inactive

C110A

residue C110 is mandatory for pyridoxal, but not for pyridoxine, or 4-amino-5-hydroxymethyl-2-methylpyrimidine turnover

C214A

mutant does not turn over pyridoxal, pyridoxine, or 4-amino-5-hydroxymethyl-2-methylpyrimidine. Residue C214 acts as the catalytic base in the phosphorylation reaction

Staphylococcus aureus ATCC 700699

C110A

residue C110 is mandatory for pyridoxal, but not for pyridoxine, or 4-amino-5-hydroxymethyl-2-methylpyrimidine turnover

C214A

Staphylococcus aureus ATCC 700699

mutant does not turn over pyridoxal, pyridoxine, or 4-amino-5-hydroxymethyl-2-methylpyrimidine. Residue C214 acts as the catalytic base in the phosphorylation reaction

additional information

Arabidopsis thaliana

Q8W1X2;

plants lacking enzyme activity display chlorosis and reduced plant size, and hypersensitivity to NaCl and sucrose. Mutant has higher level of pyridoxine, pyridoxamine, and pyridoxal 5'-phosphate. Mutant shows higher activity of pyridoxine/pyridoxamine 5'-phosphate oxidase as well as of the vitamin B6 de novo pathway enzyme Pdx1 and increased total vitamin B6 levels

694710

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

the enzyme which has lost more than 90% of the alpha-helix content after treatment with 3 M guanidine hydrochloride regains more than 90% of the original catalytic activity after overnight dialysis against 10 mM potassium phosphate, pH 7 at 4°C