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Literature summary for 2.7.1.35 extracted from

  • Wrenger, C.; Eschbach, M.L.; Muller, I.B.; Warnecke, D.; Walter, R.D.
    Analysis of the vitamin B6 biosynthesis pathway in the human malaria parasite Plasmodium falciparum (2005), J. Biol. Chem., 280, 5242-5248.
    View publication on PubMed
Search for more literature for 2.7.1.35

Application

Application Comment Organism
medicine potential target for drugs Plasmodium falciparum

Cloned(Commentary)

Cloned (Comment) Organism
expressed as Strep-tag fusion protein in Escherichia coli BLR(DE3) Plasmodium falciparum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.07
-
 pyridoxal pH 6.5, 30°C Plasmodium falciparum
0.082
-
 ATP pH 6.5, 30°C Plasmodium falciparum
0.212
-
 pyridoxine pH 6.5, 30°C Plasmodium falciparum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57200
-
 2 * 57200, calculated from the deduced amino acid sequence, 2 * 58000, SDS-PAGE, native mass by gel filtration Plasmodium falciparum
58000
-
 2 * 57200, calculated from the deduced amino acid sequence, 2 * 58000, SDS-PAGE, native mass by gel filtration Plasmodium falciparum
110000
-
 gel filtration Plasmodium falciparum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + pyridoxal Plasmodium falciparum generates the active form of vitamin B6, which serves as cofactor for many enzymes ADP + pyridoxal 5'-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Plasmodium falciparum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein using Strep-tag Plasmodium falciparum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.03
-
 with pyridoxamine as substrate, pH 6.5, 30°C Plasmodium falciparum
0.05
-
 with pyridoxal as substrate, pH 6.5, 30°C Plasmodium falciparum
0.112
-
 with pyridoxine as substrate, pH 6.5, 30°C Plasmodium falciparum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + pyridoxal
-
 Plasmodium falciparum ADP + pyridoxal 5'-phosphate
-
 ?
ATP + pyridoxal generates the active form of vitamin B6, which serves as cofactor for many enzymes Plasmodium falciparum ADP + pyridoxal 5'-phosphate
-
 ?
ATP + pyridoxamine
-
 Plasmodium falciparum ADP + pyridoxamine 5'-phosphate
-
 ?
ATP + pyridoxine
-
 Plasmodium falciparum ADP + pyridoxine 5'-phosphate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 57200, calculated from the deduced amino acid sequence, 2 * 58000, SDS-PAGE, native mass by gel filtration Plasmodium falciparum

Synonyms

Synonyms Comment Organism
PdxK
-
 Plasmodium falciparum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.03
-
 pyridoxamine pH 6.5, 30°C Plasmodium falciparum
0.05
-
 pyridoxal pH 6.5, 30°C Plasmodium falciparum
0.11
-
 pyridoxine pH 6.5, 30°C Plasmodium falciparum