Application | Comment | Organism |
---|---|---|
synthesis | production of cadaverine (1,5-diaminopentane) can be done by fermentation or direct bioconversion and plays an important role as a building block of polyamides. Lysine decarboxylase (CadA) transforms L-lysine to cadaverine and pyridoxal 5'-phosphate (PLP) can increase the conversion rate and yield as a cofactor. Biotransformation of cadaverine using whole Escherichia coli cells that overexpress lysine decarboxylase has many merits, such as the rapid conversion of L-lysine to cadaverine, possible application of high concentration reactions up to the molar level, production of less byproduct, and potential reuse of the enzyme by immobilization. But the supply of PLP, an essential cofactor of lysine decarboxylase, is the major bottleneck in this system. Among various PLP systems examined, pyridoxal kinase (PdxY) shows the highest conversion of PL to PLP, resulting in more than 60 % conversion of L-lysine to cadaverine with lysine decarboxylase. Method evaluation and optimization. Interconversion of interconvertion of pyridoxal and pyridoxamine has to be controlled | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
gene pdxY, cloned into pET23a::pdxY (p23YH) or pET23a:: pdxK (p23KH) as a one-vector system in Escherichia coli strain DH5alpha and transformed into Escherichia coli strain BL21(DE3) | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Escherichia coli | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + pyridoxal | Escherichia coli | - |
ADP + pyridoxal 5'-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P77150 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + pyridoxal | - |
Escherichia coli | ADP + pyridoxal 5'-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
pdxY | - |
Escherichia coli |
General Information | Comment | Organism |
---|---|---|
physiological function | biotransformation of pyridoxal to pyridoxal 5'-phosphate (PLP) by pyridoxal kinase (pdxY) supports cadaverine production in Escherichia coli. PLP is an essential cofactor of lysine decarboxylase and the major bottleneck in the cadaverine biosynthesis pathway | Escherichia coli |